Preparation and application of active bacterium Bacillus sp for inhibiting tobacco mildew rot

Abstract

The invention discloses preparation and application of active bacteria Bacillus sp for inhibiting the tobacco mildew rot, and relates to the technical field of microbial pesticides. A preparation process of the active bacteria Bacillus sp for inhibiting the tobacco mildew rot comprises the steps: inoculating NL-6 bacteria into a test tube LB liquid culture medium (5 ml per tube), wherein a formula of the culture medium comprises 0.5% of yeast extract, 1% of peptone, and 1% of NaCl; and inoculating the NL-6 thalli onto the culture medium, and culturing in a shaking table at the temperature of 37 DEG C for 12 hours at the rotating speed of 220rpm to obtain a test tube strain. The active bacterium Bacillus sp has the inhibitory activity on various pathogenic bacteria of the tobacco mildew rot, and after the activity of the active bacterium Bacillus sp is determined through multiple batches of activity tests for inhibiting the various pathogenic bacteria of the tobacco mildew rot, the strain is subjected to morphological, physiological, biochemical and molecular identification, and the active bacterium Bacillus sp is determined as a Bacillus sp spore bacterium.

Description

technical field [0001] The invention relates to the technical field of microbial pesticides, in particular to the preparation and application of an active fungus Bacillus sp for inhibiting tobacco mold rot. Background technique [0002] Tobacco leaves are the most important raw material for the production of cigarette products. From purchase and warehousing, threshing and redrying, to use in the production of cigarette products, they must be stored for at least 1 to 2 years. During the storage process, mildew will have a great impact on the storage of tobacco leaves. , and even partially or completely lose the use value of tobacco leaves. The longer the tobacco leaves are stored, the greater the possibility of mildew. The mildew and deterioration of tobacco leaves is caused by the infection of tobacco leaves by molds. There are molds in the natural air and on tobacco leaves and shredded tobacco. When the environmental conditions are suitable, molds will absorb nutrients suc...

AI Technical Summary

Problems solved by technology

[0003] my country's research work on smoke storage and anti-mold started late, and its scientific research and technical strength are relatively weak, which is far from providing the necessary theoretical and technical support for my country's smoke storage and anti-mold.

At present, although it can be controlled by means of physical and chemical prevention and control, these methods have certain limitations and shortcomings, and their operability and adaptability are not very good. In recent years, with the enhancement of people's environmental awareness , the application of chemical drug reagents is gradually limited. In addition to continuing to explore low-toxicity, low-residue, and high-selectivity chemical pesticides, people are more concerned about the development of preventive agents as a supplement or substitute for chemical pesticides