Complex bacillus agent as well as preparation method and application thereof

A technology of compounding Bacillus and Bacillus, applied in the field of microorganisms, can solve the problems of weakened efficacy, reduced efficacy, and reduction of beneficial bacteria in the root system, and achieves the effects of stable efficacy, cost saving and plant growth promotion.

Active Publication Date: 2020-01-21
BIOTECH CENT OF SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most domestic compound bacteria products are cultured by single bacteria, and then mixed to obtain "complex bacteria" products. The "composite bacteria" product...

Method used

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  • Complex bacillus agent as well as preparation method and application thereof
  • Complex bacillus agent as well as preparation method and application thereof
  • Complex bacillus agent as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A preparation method of a compound bacillus bacterial agent, carried out by the following steps:

[0030] (1) Inoculate the preserved methylotrophic Bacillus Bacillus methylotrophicus KNK-1, Bacillus cereus BCJB01 and Bacillus megaterium BMJBN02 into liquid LB medium respectively, and cultivate them at 30°C±1°C until In the logarithmic growth period, the concentration of the seed solution should not be lower than 1.0×10 8 cfu / mL, to obtain the seed liquid used for inoculation;

[0031] (2) Mix the methylotrophic bacillus Bacillus methylotrophicus KNK-1, Bacillus cereus BCJB01 and Bacillus megaterium BMJBN02 obtained in step (1) to obtain a mixed seed liquid, and mix the methyl The ratio of live bacteria of vegetative Bacillus methylotrophicusKNK-1, Bacillus cereus BCJB01 and Bacillus megateriumBMJBN02 is 2:2:6;

[0032] (3) Inoculate the mixed seed solution obtained in step (2) into the fermentation medium with an inoculum size of 10% (volume percentage), so that th...

Embodiment 2

[0040] Application of embodiment 2 compound bacillus inoculum in promoting capsicum industrialized seedling cultivation and production

[0041] Proceed by the following steps:

[0042] (1) Add the compound bacillus bacterial agent to the seedling-growing substrate by 10g / L, obtain the cultivating substrate after fully mixing, sow and routinely manage "Zhongjiao No. 6" according to conventional methods, and directly use the seedling-raising substrate (the Formula: the formula of seedling-raising substrate produced by Piedman Company: organic matter content ≥ 45%, humic acid content ≥ 15%, pH value: 5.5-6.8, free water content ≤ 20%) as the cultivation substrate "Zhongjiao No. 6 "Cultivation group is a negative control, and the "Zhongjiao No. 6" cultivation group that uses nitrogen, phosphorus, and potassium compound fertilizers (nitrogen: phosphorus: potassium=15:15:15) in the cultivation medium is a positive control (use amount is 60g / m 2 ), each group treated 30 plants, ...

Embodiment 3

[0051] Application of embodiment 3 compound bacillus bacterial agent in the prevention and treatment of capsicum disease (product of the present invention is compared with common pesticides in the field)

[0052] The application of a compound bacillus bacterial agent in the prevention and treatment of pepper diseases is carried out through the following steps:

[0053] (1) The subject of the test is the seedlings of the moderately sensitive variety "Zhongjiao No. 6" with the same growth in Example 2 (1). The test was carried out in the greenhouse test field of the Institute of Ecology, Shandong Academy of Sciences. The test field had pepper frost outbreaks in 2017 and 2018. Mildew, Pepper Blight, and Pepper Blight.

[0054] (2) 5 treatments in total in the test: the seedlings of "No. 6 Zhongjiao" processed by the compound bacillus inoculum in Example 2 (1) were respectively composed of 45% chlorothalonil 200 times liquid, 64% antivirus alum wettable powder 500 Times liquid, c...

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Abstract

The invention relates to the technical field of microorganisms, in particular to a preparation method and application of a complex bacillus agent. The complex bacillus agent comprises Bacillus methylotrophicus KNK-1, Bacillus cereus BCJB01 and Bacillus megaterium BMJBN02. The strains are separated and preserved in labs. The prominent characteristic of the complex bacillus agent is the applicationof the complex bacillus agent in promoting plant growth and/or controlling related plant diseases caused by plant pathogen infestation, has the growth promotion effect on plants of about 30% and has strong seedling index increased to about 92%. Besides, the complex bacillus agent has average control effect on common plant diseases up to 70% and has great application values for cultivation of economic plants.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a preparation method and application of a composite bacillus bacterial agent. Background technique [0002] Since the 21st century, the growth of population has led to a sharp increase in the demand for agricultural products. The abuse of chemical fertilizers and chemical pesticides is increasing day by day in order to increase the output of agricultural products. The abuse of chemical agents reduces the sensitivity of plant pathogens to fungicides and produces drug resistance, which leads to an increase in agricultural production costs and a reduction in the value of agricultural products; in addition, the residue of chemical agents makes the problem of farmland environmental pollution increasingly prominent. The food chain in turn threatens human health. In order to change this undesirable phenomenon, in response to the national policy of encouraging and advocating gre...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/22A01P3/00A01P21/00A01G24/20A01G22/05A01G13/00C12R1/07C12R1/085C12R1/11
CPCC12N1/20A01N63/00A01G24/20A01G22/05A01G13/00
Inventor 谢雪迎张新建周红姿周方园郭书海张广志范素素赵晓燕吴晓青
Owner BIOTECH CENT OF SHANDONG ACAD OF SCI
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