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Method for preparing Paenibacillus polymyxa biopesticide wettable powder by liquid fermentation and formulation

A polyviscous spore-like, wettable powder technology, applied in biocides, botanical equipment and methods, chemicals for biological control, etc., can solve problems such as low spore transformation rate

Active Publication Date: 2020-10-23
SHANGHAI WANLIHUA BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Simultaneously the present invention provides a kind of new liquid fermentation medium, has solved the problem of low conversion rate of traditional liquid fermentation spores

Method used

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  • Method for preparing Paenibacillus polymyxa biopesticide wettable powder by liquid fermentation and formulation
  • Method for preparing Paenibacillus polymyxa biopesticide wettable powder by liquid fermentation and formulation
  • Method for preparing Paenibacillus polymyxa biopesticide wettable powder by liquid fermentation and formulation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] The optimization of embodiment 1 culture fluid

[0058] This example is based on the basal medium (peptone: 38.4g / L, starch: 39g / L, magnesium sulfate heptahydrate: 1.05g / L, dipotassium hydrogen phosphate: 1.7g / L, glucose: 10g / L) The effects of several commonly used carbon, nitrogen sources and inorganic salts on spore production of Paenibacillus polymyxa were investigated. The impact of using different carbon sources on the number of viable bacteria and the amount of spores of Paenibacillus polymyxa in the culture medium is shown in Table 1; the impact of using different nitrogen sources on the number of viable bacteria and the amount of spores of Paenibacillus polymyxa See Table 2.

[0059] Table 1 Effect of different carbon sources on the number of viable Paenibacillus polymyxa and the amount of spores

[0060]

[0061] Table 2 Effects of different nitrogen sources on the number of viable Paenibacillus polymyxa and the amount of spores

[0062]

[0063] It ca...

Embodiment 2

[0067] The preparation of embodiment 2 Paenibacillus polymyxa fermented liquid

[0068] Carry out following operation on the basis of embodiment 1 culture fluid:

[0069] The Paenibacillus polymyxa is inoculated in the flat plate of the potato juice medium under aseptic condition, and then the flat plate is put into a constant temperature incubator, and cultivated at 35-37° C. for 3-5 days. The Paenibacillus polymyxa used in this example is Paenibacillus polymyxa P1, which has been preserved in the General Microorganism Center (CGMCC) of the China Committee for Microbial Culture Collection on January 9, 2017, and the preservation number is CGMCCNo. 13554. The medium composition of potato juice is as follows:

[0070]

[0071] Transfer the cultured Paenibacillus polymyxa to a 1L shaker flask with 300ml of culture solution under aseptic operation, place the shaker flask on a shaker, and cultivate it at 35-37°C and 200rpm for 2 days. Obtain the primary seed solution. Descr...

Embodiment 3

[0074] Example 3 Preparation of Paenibacillus polymyxa biopesticide parent drug and Paenibacillus polymyxa biopesticide wettable powder

[0075] Paenibacillus polymyxa fermented liquid 500L that embodiment 2 makes is fully mixed solid-liquid mixture with 10Kg sodium alginate, 10Kg chitosan, 10Kg sucrose, 20Kg glycerin, 10Kg white carbon black, 30Kg diatomaceous earth;

[0076] The solid-liquid mixture is subjected to plate and frame pressure filtration under a pressure of 0.2Mpa to obtain a filter cake containing Bacillus polymyxa;

[0077] Filter cake at an inlet air temperature of 120°C and an induced draft fan air volume of 8000m 3 / hour, under the condition of feeding rate 25Kg / hour, through flash drying, obtain Paenibacillus polymyxa biopesticide master drug. The spore content in the Paenibacillus polymyxa biological pesticide master drug is 20 billion cfu / g, and the water content is 15%.

[0078] The above-mentioned Paenibacillus polymyxa biopesticide master drug and...

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Abstract

The invention discloses a method used for preparing a Paenibacillus polymyxa biological pesticide wettable powder based on liquid fermentation and a formula dosage form. A Paenibacillus polymyxa brothadopted in the method is capable of solving a problem that the spore conversion rate of conventional liquid fermentation is low, and the spore conversion rate of the method is higher than 98%. The obtained Paenibacillus polymyxa broth is composed of active Paenibacillus polymyxa; the stress resistance is excellent; the service life is long; long time storage can be realized; self life is long; the method is suitable for fermentation technology, and industrialized production. The invention also discloses a production method of a Paenibacillus polymyxa concentrate and the Paenibacillus polymyxabiological pesticide wettable powder; the preparation method is simple; spore recovery rate is 98% or higher; a plurality of spore protection technologies are adopted; product self life is prolongedto be 18months or longer; and commercial value is achieved.

Description

technical field [0001] The invention relates to a method for preparing biopesticide, in particular to a method for preparing Paenibacillus polymyxa biopesticide wettable powder by liquid fermentation and formulation. Background technique [0002] Plant disease is one of the main obstacles restricting the safe growth of plants. The current prevention and control method is mainly spraying chemical agents. Vegetable crops have a short growth period, and the frequent use of chemical agents has the risk of pesticide residues. The development and use of biological pesticides can reduce the amount of chemical agents used. Microorganisms that have antagonistic effects on plant pathogenic bacteria are the main source for the development of biopesticides. Such microorganisms are diverse and widely distributed. Even if the same microorganism is distributed in different regions, the strength or mechanism of the antagonistic effect is also different. To mine biocontrol microbial resourc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/25A01N25/14A01P3/00
CPCA01N25/14A01N63/10
Inventor 陈晨旷文丰许滢陈娟吴紫燕毛伟力
Owner SHANGHAI WANLIHUA BIOTECH
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