The invention relates to a method for synthesizing a glassine. The method comprises the following steps: reacting (2R, 3S, 4S)-2, 3, 4-tribenzyloxy glutaraldehyde with acetone to synthesize a key intermediate (E)-(2R, 3S, 4S)-2, 3, 4-tribenzyloxy pentyl-6-acetyl hexane-5-olefine aldehyde, then catalyzing (E)-(2R, 3S, 4S)-2, 3, 4-tribenzyloxy pentyl-6-acetyl hexane-5-olefine aldehyde with Lewis acid to sequentially carry out a nucleophilic addition and a 1, 4-Michael addition reactions, obtaining benzyl-protected Pro-Xylane<TM> 1-((2S, 3S, 4S, 5R)-3, 4, 5-tri(benzyloxy) tetrahydro-2H-pyran-2-yl)propan-2-ol, and finally, carrying out catalytic hydrogenation by using palladium carbon to remove benzyl, so as to obtain Pro-XylaneTM (2S, 3R, 4S, 5R)-2-(2-hydroxypropyl) tetrahydro-2H-pyran-3, 4, 5-triol. The method is high in reaction yield, the obtained product is high in purity, and the method has huge application value.