Preparation method of bacillus amyloliquefaciens G5 spore bacteria powder

A technology of amyloliquefaciens spores and spores, which is applied in the field of preparation of Bacillus amyloliquefaciens G5 spore powder, and can solve problems such as unsatisfactory preparation methods of spore powder

Inactive Publication Date: 2018-06-12
SHANDONG SUKAHAN BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the preparation method of Bacillus amyloliquefaciens spore powder at present stage is all not ideal

Method used

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  • Preparation method of bacillus amyloliquefaciens G5 spore bacteria powder
  • Preparation method of bacillus amyloliquefaciens G5 spore bacteria powder

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preparation example Construction

[0029] The invention provides a preparation method of Bacillus amyloliquefaciens G5 spore powder, comprising the steps of:

[0030] The first step is to prepare the spore-forming medium

[0031] The raw materials of the spore-forming medium are: carbon source, nitrogen source and inorganic salt;

[0032] The components of the spore-forming medium are: cornstarch 10-40g / L, glucose 0.5-3g / L, soybean meal 5-25g / L, calcium carbonate 0.1-1.5g / L, ammonium chloride 0.1-1.0g / L L. Dipotassium hydrogen phosphate 0.1-1.0g / L, magnesium sulfate 0.01-0.7g / L and manganese sulfate 0.01-0.7g / L;

[0033] Pour the components of the spore-forming medium into a fermenter, add water to adjust the concentration; then adjust the pH to 7.5-7.8, and then sterilize at 121-123° C. to obtain the spore-forming medium;

[0034] The second step of cultivating spore fermentation broth

[0035] Under sterile conditions, transfer the seed solution of Bacillus amyloliquefaciens G5 into the fermenter at a volu...

Embodiment 1

[0054] The first step is to prepare the spore-forming medium

[0055] The components of the spore producing medium are: carbon source, nitrogen source and inorganic salt; the carbon source raw material is cornstarch and glucose; the nitrogen source raw material is soybean meal; the inorganic salt raw material is calcium carbonate, ammonium chloride, phosphoric acid Dipotassium hydrogen, magnesium sulfate and manganese sulfate;

[0056] Pour the components of the spore-producing medium into a fermenter; add water to adjust the concentration of the components of the spore-producing medium: cornstarch 15g / L, glucose 1g / L, soybean meal 10g / L, calcium carbonate 0.4g / L, Ammonium Chloride 0.2g / L, Dipotassium Hydrogen Phosphate 0.5g / L, Magnesium Sulfate 0.3g / L and Manganese Sulfate 0.2g / L; then adjust the pH to 7.5~7.8, and then under the condition of 121~123℃ Sterilize at high temperature; obtain spore-forming medium.

[0057] After cultivating Bacillus amyloliquefaciens G5 with t...

Embodiment 2

[0059] The first step is to prepare the spore-forming medium

[0060] The components of the spore producing medium are: carbon source, nitrogen source and inorganic salt; the carbon source raw material is cornstarch and glucose; the nitrogen source raw material is soybean meal; the inorganic salt raw material is calcium carbonate, ammonium chloride, phosphoric acid Dipotassium hydrogen, magnesium sulfate and manganese sulfate;

[0061] Pour the components of the spore-producing medium into a fermenter; add water to adjust the concentration of each component of the spore-producing medium: cornstarch 20g / L, glucose 2.3g / L, soybean meal 8g / L, calcium carbonate 0.5 g / L, 0.8g / L ammonium chloride, 0.4g / L dipotassium hydrogen phosphate, 0.1g / L magnesium sulfate and 0.2g / L manganese sulfate; Sterilize under high temperature; obtain spore-forming medium.

[0062] After cultivating Bacillus amyloliquefaciens G5 with the spore-producing medium of the above-mentioned concentration, the ...

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Abstract

The invention is applied to the technical field of microbial fermentation and provides a preparation method of bacillus amyloliquefaciens G5 spore bacteria powder. The method comprises the following steps: step one, preparing a spore-producing culture medium from corn starch, glucose, soybean meal, calcium carbonate, ammonium chloride, dipotassium phosphate, magnesium sulfate and manganese sulfate; step two, culturing a spore fermentation solution; and step four, preparing spore bacteria powder by adding ammonium sulfate and kieselguhr into the spore fermentation solution and then carrying outplate-frame filtration to obtain the bacillus amyloliquefaciens G5 spore bacteria powder. In the preparation method of the bacillus amyloliquefaciens G5 spore bacteria powder, the raw materials of the spore-producing culture medium are common, convenient and easily available, so that the production cost is reduced; a large number of spores exist in the obtained spore fermentation solution; the spore yield is greater than or equal to 98%; ammonium sulfate and kieselguhr are taken as flocculants, so that the preparation time of the spore bacteria powder is reduced; a large number of effective living bacteria exist in the obtained spore bacteria powder; the yield of the effective living bacteria is greater than or equal to 95%.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a preparation method of Bacillus amyloliquefaciens G5 spore powder. Background technique [0002] Bacillus amyloliquefaciens is widely distributed in nature as a kind of probiotics. It is non-toxic and harmless to humans and animals, does not pollute the environment, grows fast, has good stability, has broad-spectrum antibacterial activity and strong stress resistance. Spores, also known as endospores, are bacterial dormant bodies; spore-forming bacteria form round or oval spore dormant bodies in cells when nutrient conditions are lacking. Spores have extremely low water content, strong stress resistance, and can withstand high temperature, ultraviolet rays, ionizing radiation, and various chemical substances. When conditions are suitable, spores will become bacteria again; because spores are easier to preserve and revive than their vegetative cells It has a high ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N3/00C12R1/07
CPCC12N1/20C12N3/00
Inventor 郭芳先肖发沂陈永科韩威华刘镇韩岳
Owner SHANDONG SUKAHAN BIO TECH
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