Preparation method of bacillus amyloliquefaciens G5 spore bacteria powder
A technology of amyloliquefaciens spores and spores, which is applied in the field of preparation of Bacillus amyloliquefaciens G5 spore powder, and can solve problems such as unsatisfactory preparation methods of spore powder
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[0029] The invention provides a preparation method of Bacillus amyloliquefaciens G5 spore powder, comprising the steps of:
[0030] The first step is to prepare the spore-forming medium
[0031] The raw materials of the spore-forming medium are: carbon source, nitrogen source and inorganic salt;
[0032] The components of the spore-forming medium are: cornstarch 10-40g / L, glucose 0.5-3g / L, soybean meal 5-25g / L, calcium carbonate 0.1-1.5g / L, ammonium chloride 0.1-1.0g / L L. Dipotassium hydrogen phosphate 0.1-1.0g / L, magnesium sulfate 0.01-0.7g / L and manganese sulfate 0.01-0.7g / L;
[0033] Pour the components of the spore-forming medium into a fermenter, add water to adjust the concentration; then adjust the pH to 7.5-7.8, and then sterilize at 121-123° C. to obtain the spore-forming medium;
[0034] The second step of cultivating spore fermentation broth
[0035] Under sterile conditions, transfer the seed solution of Bacillus amyloliquefaciens G5 into the fermenter at a volu...
Embodiment 1
[0054] The first step is to prepare the spore-forming medium
[0055] The components of the spore producing medium are: carbon source, nitrogen source and inorganic salt; the carbon source raw material is cornstarch and glucose; the nitrogen source raw material is soybean meal; the inorganic salt raw material is calcium carbonate, ammonium chloride, phosphoric acid Dipotassium hydrogen, magnesium sulfate and manganese sulfate;
[0056] Pour the components of the spore-producing medium into a fermenter; add water to adjust the concentration of the components of the spore-producing medium: cornstarch 15g / L, glucose 1g / L, soybean meal 10g / L, calcium carbonate 0.4g / L, Ammonium Chloride 0.2g / L, Dipotassium Hydrogen Phosphate 0.5g / L, Magnesium Sulfate 0.3g / L and Manganese Sulfate 0.2g / L; then adjust the pH to 7.5~7.8, and then under the condition of 121~123℃ Sterilize at high temperature; obtain spore-forming medium.
[0057] After cultivating Bacillus amyloliquefaciens G5 with t...
Embodiment 2
[0059] The first step is to prepare the spore-forming medium
[0060] The components of the spore producing medium are: carbon source, nitrogen source and inorganic salt; the carbon source raw material is cornstarch and glucose; the nitrogen source raw material is soybean meal; the inorganic salt raw material is calcium carbonate, ammonium chloride, phosphoric acid Dipotassium hydrogen, magnesium sulfate and manganese sulfate;
[0061] Pour the components of the spore-producing medium into a fermenter; add water to adjust the concentration of each component of the spore-producing medium: cornstarch 20g / L, glucose 2.3g / L, soybean meal 8g / L, calcium carbonate 0.5 g / L, 0.8g / L ammonium chloride, 0.4g / L dipotassium hydrogen phosphate, 0.1g / L magnesium sulfate and 0.2g / L manganese sulfate; Sterilize under high temperature; obtain spore-forming medium.
[0062] After cultivating Bacillus amyloliquefaciens G5 with the spore-producing medium of the above-mentioned concentration, the ...
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