Biological deodourant and preparation method thereof
The technology of biological deodorant and plant extract is applied in the field of biological deodorant and its preparation, which can solve the problems of high price of biological deodorant, long deodorization time, long preparation period, etc., so as to inhibit the breeding of harmful bacteria. , the effect of speeding up the decomposition speed and low cost of preparation materials
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Embodiment 1
[0024] The biological deodorant described in this embodiment is yeast liquid, spore liquid, lactic acid bacteria liquid, photosynthetic bacteria liquid and active enzyme liquid, the mass ratio of yeast liquid, spore liquid, lactic acid bacteria liquid, photosynthetic bacteria liquid, active enzyme liquid It is 1.5:1.5:1.5:0.07:0.005.
[0025] The fermentation conditions of the yeast liquid and the spore liquid are as follows: a ventilated stirring fermenter is used; medium sterilization temperature and time: 121°C, 30min; culture conditions are: ventilation rate 0.1vvm, stirring rate 150r / min, culture temperature 30°C, culture time 24h; Yeast adopts YPD medium, pH 5; Bacillus culture uses LB medium, pH 7. The number of yeast cells in the yeast liquid prepared in this embodiment is 8×10 4 / mL, the number of spore cells in the prepared spore liquid was 5×10 5 / mL.
[0026] The fermentation conditions of the photosynthetic bacteria liquid are as follows: use an anaerobic seale...
Embodiment 2
[0031] The biological deodorant described in this embodiment is yeast liquid, spore liquid, lactic acid bacteria liquid, photosynthetic bacteria liquid and active enzyme liquid, the mass ratio of yeast liquid, spore liquid, lactic acid bacteria liquid, photosynthetic bacteria liquid, active enzyme liquid is 1.2:1.2:1.2:0.06:0.003.
[0032] The fermentation conditions of the yeast liquid and spore liquid are as follows: a ventilated stirring fermenter is used; medium sterilization temperature and time: 121°C, 30min; culture conditions are: ventilation rate 0.3vvm, stirring rate 180r / min, culture temperature 33°C, culture time 36h; Yeast adopts YPD medium, pH 6; Bacillus culture uses LB medium, pH 8. The number of yeast cells in the yeast liquid prepared in this embodiment is 7.5×10 4 / mL, the number of spore cells in the prepared spore liquid was 5×10 5 / mL.
[0033] The fermentation conditions of the photosynthetic bacteria liquid are as follows: use an anaerobic sealed fer...
Embodiment 3
[0038] The biological deodorant described in this embodiment is yeast liquid, spore liquid, lactic acid bacteria liquid, photosynthetic bacteria liquid and active enzyme liquid, the mass ratio of yeast liquid, spore liquid, lactic acid bacteria liquid, photosynthetic bacteria liquid, active enzyme liquid It is 1:1:1:0.05:0.001.
[0039] The fermentation conditions of the yeast liquid and the spore liquid are as follows: a ventilated stirring fermenter is used; medium sterilization temperature and time: 121°C, 30min; culture conditions are: ventilation rate 0.5vvm, stirring rate 200r / min, culture temperature 37°C, culture time 48h; Yeast adopts YPD medium, pH 5.5; Bacillus culture uses LB medium, pH 7.5. The number of yeast cells in the yeast liquid prepared in this embodiment is 8×10 4 / mL, the number of spore cells in the prepared spore liquid was 5×10 5 / mL.
[0040] The fermentation conditions of the photosynthetic bacteria liquid are as follows: use an anaerobic sealed ...
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