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Preparation method of immobilized barley beta-amylase taking reconstructed macroporous molecular sieve as carrier thereof

A macroporous molecular sieve and amylase technology, which is applied in the direction of being immobilized on or in an inorganic carrier, can solve the problems such as the immobilization conditions of immobilized β-amylase are not specified, and achieve good maltose quality, stable operation and preparation technology. simple effect

Inactive Publication Date: 2010-01-13
郸城财鑫糖业有限责任公司
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Problems solved by technology

[0004] The "molecular sieve immobilized glucoamylase and its preparation method" reported in Chinese patent CN 127298 also adopts the method of adsorption and cross-linking to prepare immobilized enzyme, but it is used for the production of glucose products, and does not describe the immobilization of immobilized β-amylase conditions, and the molecular sieve used for immobilizing β-amylase is essentially different from the present invention in characteristics

Method used

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  • Preparation method of immobilized barley beta-amylase taking reconstructed macroporous molecular sieve as carrier thereof

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Embodiment

[0020] The present invention utilizes the preparation method of forming molecular sieve immobilized barley β-amylase, specifically comprises the following steps:

[0021] (1) Production of molecular sieves:

[0022] Molecular sieves use β-zeolite as the matrix, and undergo pore expansion to obtain large-pore molecular sieves. Pseudoboehmite is used as the binder, mixed and pressed, dried at room temperature, roasted, crushed into granules, and passed through a 20-40 mesh sieve. , uniform particle size.

[0023] (2) Preparation of reaction column

[0024] Put molecular sieves with uniform particle size into a reaction column with an insulating layer, and place supports or filter screens at the bottom of the column.

[0025] (3) Preparation of enzyme solution

[0026] The barley β-amylase is diluted with a buffer solution with a pH of about 5-5.5 to prepare an enzyme solution with an enzyme activity of 20,000-40,000 units.

[0027] (4) The first adsorption

[0028] Take an ...

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Abstract

The invention relates to a preparation method used for producing the immobilized enzyme of malt syrup, in particular to a preparation method of immobilized barley beta-amylase taking reconstructed macroporous molecular sieve as the carrier thereof. The method takes the reconstructed macroporous molecular sieve as the carrier, adopts the method of multiple adsorb crosslinking, and prepares stable immobilized barley beta-amylase. The method has the advantages that the production technology of immobilized enzyme is simple, the condition is mild, multiple adsorption and crosslinking can be performed, the required amount of enzyme is small, the quantity of hanging enzyme is large, the mechanical property is good, the operation is stable, the stability of the immobilized enzyme is good, the quality of the produced malt sugar is good, and the carrier can be repeatedly used, and the method is applicable to the packed bed reactors.

Description

technical field [0001] The invention relates to a preparation method of immobilized enzyme for producing maltose syrup, in particular to a preparation method of immobilized barley beta-amylase with reconstituted macroporous molecular sieve as carrier. Background technique [0002] Maltose is one of the low-sweetness and low-calorie sugars. Maltose has mild sweetness, delicious fragrance and no aftertaste after eating. It is a new type of food sweetener and sugar substitute. Due to its unique function in the human body, maltose is widely used in fields such as medicine and food. At present, the production of maltose is a double-enzyme method. Since the saccharification tank is required to maintain a certain reaction time, the production process is semi-continuous, so the investment in equipment and the occupied space are relatively large. The use of immobilized enzyme technology can effectively save production Cost and reaction time, and the process is continuous, which has ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14
Inventor 仵树仁李霄峰叶新友李锦王念详陆启明闫锁王金恒赵严博张旗
Owner 郸城财鑫糖业有限责任公司
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