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Preparation method for immobilized alpha-amylase with graphene/titanium oxide composite porous microspheres as carrier

A technology of porous microspheres and graphene, applied in biochemical equipment and methods, chemical instruments and methods, alkali metal oxides/hydroxides, etc., can solve the difficulties of product separation and purification, enzyme loss of activity, not a catalyst, etc. problems, to achieve the effect of excellent repeated use performance, good stability and good thermal stability

Inactive Publication Date: 2017-10-24
DONGGUAN LIANZHOU INTPROP OPERATION MANAGEMENT CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the high-level structure of the enzyme is very sensitive to the environment, and various physical, chemical and biological factors may cause the enzyme to lose its activity, and the enzyme used in the reaction can only be used once, and the separation and purification of the reaction products are relatively difficult. Relatively high cost of production
For modern industry, pure biological enzyme is not an ideal catalyst

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A preparation method of immobilized α-amylase using graphene / titanium oxide composite porous microspheres as a carrier, comprising the following steps:

[0025] (1) After the graphene oxide and deionized water are mixed and stirred evenly, the graphene oxide solution is obtained by ultrasonic treatment for 1 hour at a power of 500W; wherein, the mass concentration of the graphene oxide solution is 5 mg / g;

[0026] (2) Using a syringe, add the above-mentioned graphene oxide solution dropwise into the receiving liquid controlled by a low-temperature freezing bath, and then undergo freeze-drying and vacuum drying at 100° C. to obtain porous graphene oxide balls; The temperature is -30°C; the freeze-drying condition is -5°C, and the freeze-drying time is 1 day;

[0027] (3) Mix and stir cyclohexane and absolute ethanol evenly, then add oleic acid, and after stirring for 30 minutes, add tetrabutyl titanate and triethylamine dropwise into the mixed solution, continue stirring...

Embodiment 2

[0031] A preparation method of immobilized α-amylase using graphene / titanium oxide composite porous microspheres as a carrier, comprising the following steps:

[0032] (1) After the graphene oxide and deionized water are mixed and stirred evenly, the graphene oxide solution is obtained by ultrasonic treatment for 5 hours at a power of 500W; wherein, the mass concentration of the graphene oxide solution is 32mg / g;

[0033] (2) Using a syringe, add the above-mentioned graphene oxide solution dropwise into the receiving liquid controlled by a low-temperature freezing bath, and then undergo freeze-drying and vacuum drying at 100° C. to obtain porous graphene oxide balls; The temperature is -196°C; the freeze-drying condition is -50°C, and the freeze-drying time is 5 days;

[0034] (3) Mix and stir cyclohexane and absolute ethanol evenly, then add oleic acid, and after stirring for 30 minutes, add tetrabutyl titanate and triethylamine dropwise into the mixed solution, continue stir...

Embodiment 3

[0038] A preparation method of immobilized α-amylase using graphene / titanium oxide composite porous microspheres as a carrier, comprising the following steps:

[0039] (1) After the graphene oxide and deionized water are mixed and stirred evenly, the graphene oxide solution is obtained by ultrasonic treatment for 2 hours at a power of 500W; wherein, the mass concentration of the graphene oxide solution is 10mg / g;

[0040] (2) Using a syringe, add the above-mentioned graphene oxide solution dropwise into the receiving liquid controlled by a low-temperature freezing bath, and then undergo freeze-drying and vacuum drying at 100° C. to obtain porous graphene oxide balls; The temperature is -80°C; the freeze-drying condition is -10°C, and the freeze-drying time is 2 days;

[0041] (3) Mix and stir cyclohexane and absolute ethanol evenly, then add oleic acid, and after stirring for 30 minutes, add tetrabutyl titanate and triethylamine dropwise into the mixed solution, continue stirr...

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PUM

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Abstract

The invention discloses a preparation method for immobilized alpha-amylase with graphene / titanium oxide composite porous microspheres as a carrier. The preparation method particularly includes the steps of: 1) preparing the graphene / titanium oxide composite porous microspheres; 2) preparing an alpha-amylase solution, mixing and stirring the graphene / titanium oxide composite porous microspheres with the alpha-amylase solution for 1-2 h, and performing ultrasonic treatment for 1-4 h at the power of 300 W, and filtering the liquid to obtain a solid; 3) washing the solid with a buffer solution composed of citric acid and sodium citrate, and drying the solid to obtain the immobilized alpha-amylase. The immobilized alpha-amylase is low in deactivation, has excellent repeatability, and is good in thermo-stability.

Description

Technical field: [0001] The invention relates to the field of preparation of enzyme preparations, in particular to a preparation method of immobilized α-amylase using graphene / titanium oxide composite porous microspheres as a carrier. Background technique: [0002] α-amylase is ubiquitous in animals, plants and microorganisms, it can cut off the α-1,4 glucosidic bonds in starch, glycogen, oligosaccharides or polysaccharide molecules, and produce maltose, oligosaccharides and glucose, etc. The product is widely used in various industries such as food processing, grain industry, alcohol industry, fermentation industry and textile industry, and is one of the most widely used enzyme preparations in industrial production. However, the high-level structure of the enzyme is very sensitive to the environment, and various physical, chemical and biological factors may cause the enzyme to lose its activity, and the enzyme used in the reaction can only be used once, and the separation a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14B01J20/20B01J20/28
CPCC12N11/14B01J20/06B01J20/20B01J20/28019C12N9/2414C12Y302/01001
Inventor 陈东进
Owner DONGGUAN LIANZHOU INTPROP OPERATION MANAGEMENT CO LTD
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