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Wide-temperature amylase production method

A production method and technology of amylase, applied in the field of bioengineering, can solve problems such as wide temperature amylase wide temperature amylase production method not reported and the like

Inactive Publication Date: 2012-08-01
无锡德冠生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0028] And the bacterial classification that can produce wide temperature amylase and the production method of wide temperature amylase do not have report

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Bevel formula (calculated in g / L)

[0050] Beef juice 4, peptone 10, yeast powder (yeast extract) 3, NaCl 3, starch 10, glucose 50, agar 15-25, culture temperature 35°C, culture time: 30h.

[0051] Shake bottle formula (calculated in g / L)

[0052] Bean cake powder 40, cottonseed cake powder 30, (NH 4 ) 2 SO 4 5. CaCl 2 0.5, corn steep liquor 30, lactose 150, sodium citrate 5, dipotassium hydrogen phosphate 15, potassium dihydrogen phosphate 7, culture temperature 40°C, culture time 30h.

[0053] Seed tank formula (calculated in g / L)

[0054] Glucose 75, yeast powder 30, cottonseed cake powder 15, ammonium sulfate 5, sodium citrate 8, CaCl 2 0.5, P.P.E. (polyether defoamer) 0.5, dipotassium hydrogen phosphate 7, potassium dihydrogen phosphate 7, fermentation temperature 35°C, ventilation 0.8 V.V.M, tank pressure 0.8 kg / cm 2 , Time 36 hours, seed tank volume: 500L, 60% charge.

[0055] Production tank formula (calculated in g / L)

[0056] Glucose 75, yeast pow...

Embodiment 2

[0059] The broad temperature amylase that embodiment 1 makes, the relation of its temperature of reaction and reaction efficiency is as figure 1 shown in the curve. It is shown that the action temperature range of the amylase can be 30-100°C.

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Abstract

A wide-temperature amylase production method belongs to the technical field of bioengineering. A strain used in the method is BL-mut08 (bacillus licheniformis-mut08), which is preserved in CCTCC (china center for type culture collection) and encoded as CCTCC NO.M207193, and is disclosed in China patent 200810020635.2. By means of compound mutation, preservation, rejuvenation and breeding of an original BL system and optimizing a medium formula and fermentation process conditions, the method of producing wide-temperature amylase is established, and the amylase produced has a wide range of an action temperature and high heat resistance. The strain reaches a peak of amylase production after being cultured for 72 hours at 37 DEG C. fermentation liquor is flocculated, press-filtered, super-filtered and concentrated to obtain the concentrated liquid, and different amylase preparations, such as pellets, particles or powder, are obtained by other post-processing methods. The amylase preparation is widely applicable to food and medicine industries, especially textile industry, paper industry and industries of alcoholic fermentation, monosodium glutamate, antibiotics and the like.

Description

field of invention [0001] The invention discloses a production method of wide-temperature amylase, which belongs to the technical field of bioengineering. Background technique [0002] Amylase is the most successful industrial application of enzyme preparations, because enzymatic hydrolysis can control starch hydrolysis, reaction specificity and product stability to a greater extent. Mild reaction conditions include lower temperature, near-neutral pH, etc., thereby reducing energy consumption in production, reducing production cost and environmental pollution. It can increase the output and bring various advantages such as beneficial economic benefits and social benefits. [0003] Amylase has been widely used in the desizing of the textile industry, the treatment of starch pulp in the paper industry, the food industry such as caramel, beer, rice wine, glucose, monosodium glutamate, antibiotics and other industries; the raw material treatment of the alcohol industry has a ve...

Claims

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Application Information

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IPC IPC(8): C12N9/28C12R1/10
Inventor 郁磊陶丁伟邹联
Owner 无锡德冠生物科技有限公司
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