Compound amylase preparation and its application in starch liquefaction and starch liquefaction method

A technology of compound starch and starch liquefaction, applied in biochemical equipment and methods, enzymes, fermentation, etc., can solve the problems of high viscosity of feed liquid, low benefit, incomplete liquefaction, etc., to increase the rate, increase the degree of liquefaction, Favorable Diffusion Effect

Active Publication Date: 2020-02-07
COFCO GROUP +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a compound amylase preparation and its In the application of starch liquefaction and the method of starch liquefaction, the compound amylase preparation can not only quickly reduce the viscosity of the system when the concentration of the starch feed liquid is high, that is, under the condition of relatively low water activity, and make the feed liquid mix evenly, It is good for pumping and has good thermal stability. It can be sprayed and liquefied at a higher temperature to improve the degree of liquefaction of starch feed liquid.

Method used

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  • Compound amylase preparation and its application in starch liquefaction and starch liquefaction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Configuration concentration is the fresh cornstarch feed liquid of 48% by weight, uses the HCl of 1mol / L to adjust pH to 5.6; Takes 20g above-mentioned feed liquids in reaction kettle, adds the compound amylase preparation (S type amylase) of 15U / gDS The enzymatic ratio of amylase and L-type amylase is 20:1), mix evenly, and then heat the oil bath to 115°C for spray liquefaction, and keep it for 8 minutes; 120min.

[0048] The DE value and sugar profile distribution (DP) of the reaction product were measured, and the results are shown in Table 2.

[0049] The measured pH value is 5.6, the peak value after mixing the fresh cornstarch feed liquid with a concentration of 48% by weight and the compound amylase preparation of 15U / gDS (the enzyme activity ratio of S-type amylase and L-type amylase is 20:1) Viscosity and final value viscosity, the results are shown in Table 3.

Embodiment 2

[0051] Configuration concentration is the fresh cornstarch feed liquid of 46% by weight, uses the HCl of 1mol / L to adjust pH to 4.5; Takes 20g above-mentioned feed liquids in reaction kettle, adds the compound amylase preparation (S type amylase) of 10U / gDS The enzymatic ratio of amylase and L-type amylase is 15:1), mix evenly, and then heat the oil bath to 110°C for spray liquefaction, and keep it for 5 minutes; 115min.

[0052] The DE value and sugar profile distribution (DP) of the reaction product were measured, and the results are shown in Table 2.

[0053] The measured pH value is 4.5, and the peak value after mixing the fresh corn starch feed liquid with a concentration of 46% by weight and the compound amylase preparation of 15U / gDS (the enzyme activity ratio of S-type amylase and L-type amylase is 15:1) Viscosity and final value viscosity, the results are shown in Table 3.

Embodiment 3

[0055] Configuration concentration is the fresh cornstarch feed liquid of 50% by weight, uses the HCl of 1mol / L to adjust pH to 6; Takes 20g above-mentioned feed liquids in the reactor, adds the compound amylase preparation (S type amylase) of 25U / gDS The enzymatic ratio of amylase and L-type amylase is 25:1), mix evenly, and then heat the oil bath to 120°C for spray liquefaction, and keep it for 10 minutes; 100min.

[0056] The DE value and sugar profile distribution (DP) of the reaction product were measured, and the results are shown in Table 2.

[0057] The measured pH value is 6, the peak value after mixing the fresh cornstarch feed liquid with a concentration of 50% by weight and the compound amylase preparation of 25U / gDS (the enzyme activity ratio of S-type amylase and L-type amylase is 25:1) Viscosity and final value viscosity, the results are shown in Table 3.

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Abstract

The invention relates to the field of starch sugar preparation, and discloses a compound amylase preparation, an application thereof in starch liquefaction and a starch liquefaction method. The compound amylase preparation comprises L-type amylase and S-type amylase, wherein the amino acid sequence of the L-type amylase has at least 95% sequence consistency with the amino acid sequence shown in SEQ ID NO: 1, and the amino acid sequence of the S-type amylase has at least 95% sequence consistency with the amino acid sequence shown in SEQ ID NO: 2. The method comprises the following steps of: adding the compound amylase preparation into starch feed liquid, and spraying. The compound amylase preparation can rapidly reduce the viscosity of a system in high-concentration starch feed liquid (under the condition of relatively low water activity), enables the feed liquid to be uniformly mixed, is favorable for pumping, has better thermal stability, can be sprayed and liquefied at a higher temperature, enables starch particles to be completely destroyed, and is favorable for complete liquefaction.

Description

technical field [0001] The invention relates to the field of starch sugar production, in particular to a compound amylase preparation and its application in starch liquefaction and a starch liquefaction method. Background technique [0002] Starch sugar is a bulk product produced from starch rich in grains, potatoes, etc., under the catalysis of enzymes, through a series of reactions such as liquefaction and saccharification. Starch sugar has many functions. It can be used not only as a food additive, but also as an important industrial raw material. It can also be further processed into products such as oligosaccharides, vitamins, amino acids, etc. These products are closely related to people's lives and play a role in the national economy important position. [0003] At present, the starch sugar production process generally adopts a double-enzyme method, which includes enzyme liquefaction and saccharification processes. Among them, liquefaction is the rapid expansion of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/26C12P19/14
Inventor 佟毅章方良李义周旭许宏贤彭辉白挨玺周鹏陶进
Owner COFCO GROUP
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