Preparing purification method for thermophilic alpha-amylase

A technology of amylase and thermophilic archaea, applied in the field of bioengineering
CN101063115AInactive Publication Date: 2007-10-31SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
0 Cites 6 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
Publication Date
2007-10-31
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a preparing method of thermophilic alpha-amylase (PFA), which comprises the following steps: heating and dissolving recombinant protein inclusion body under the condition of alkalinity; diluting; compositing; separating and purifying through column layer. This method possesses high reclaiming ratio and high alpha-amylase activity.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the technical field of bioengineering, in particular to the extraction and purification of recombinant proteins and thermophilic enzymes. Background technique

[0002] High temperature resistant α-amylase is one of the most commonly used enzyme preparations in industry, and is widely used in starch processing in food industry and chemical industry. The search and development of high heat-tolerant amylases has long been a key and hot topic in the field of industrial enzyme research. The global starch processing enzyme market is about 150 million US dollars, of which about 24% are used in the starch liquefaction process. Therefore, any improvement in amylase production yield, heat resistance and enzyme activity has very important theoretical significance and direct commercial value.

[0003] At present, the heat-resistant amylase commonly used in industry comes from Bacillus licheniformis. The optimum catalytic temperature of t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More