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49 results about "Alpha-amylase activity" patented technology
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Catalysis of the endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more alpha-(1->4)-linked D-glucose units. [EC:3.2.1.1]
Alpha-amylase mutants
InactiveUS6440716B1Improve stabilitySpecific activityOrganic detergent compounding agentsEnzymesMutantAlpha-amylase activity
The present invention relates to a method of constructing a variant of a parent Termamyl-like alpha-amylase, which variant has alpha-amylase activity and at least one altered property as compared to the parent alpha-amylase, comprisesi) analysing the structure of the parent Termamyl-like alpha-amylase to identify at least one amino acid residue or at least one structural part of the Termamyl-like alpha-amylase structure, which amino acid residue or structural part is believed to be of relevance for altering the property of the parent Termamyl-like alpha-amylase (as evaluated on the basis of structural or functional considerations),ii) constructing a Termamyl-like alpha-amylase variant, which as compared to the parent Termamyl-like alpha-amylase, has been modified in the amino acid residue or structural part identified in i) so as to alter the property, and, optionally,iii) testing the resulting Termamyl-like alpha-amylase variant with respect to the property in question.
Owner:NOVOZYMES AS
Amylase variants
The present invention relates to variants (mutants) of polypeptides, in particular Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits an alteration in at least one of the following properties relative to said parent alpha-amylase: substrate specificity, substrate binding, substrate cleavage pattern, thermal stability, pH / activity profile, pH / stability profile, stability towards oxidation, Ca2+ dependency, specific activity, and solubility, in particular under production conditions.
Owner:NOVOZYMES AS
Enzymes having alpha amylase activity and methods of use thereof
InactiveUS7323336B2Good fresh-keeping effectLess crumb firmingBacteriaSugar derivativesNucleotideIncrease ph
The invention relates to alpha amylases and to polynucleotides encoding the alpha amylases. In addition methods of designing new alpha amylases and methods of use thereof are also provided. The alpha amylases have increased activity and stability at increased pH and temperature.
Owner:BASF ENZYMES
Thermostable alpha-amylases
ActiveUS7189552B2Quick responseHigh resultSugar derivativesBacteriaEscherichia coliOpen reading frame
An isolated polynucleotide comprising an open reading frame encoding a polypeptide having alpha-amylase activity, the polypeptide selected from the group consisting of: a) a polypeptide comprising an amino acid sequence which has at least 70% identity with amino acids 22 to 450 of SEQ ID NO:4; b) a polypeptide comprising an amino acid sequence which has at least 70% identity with the polypeptide encoded by the amylase encoding part of the polynucleotide inserted into a plasmid present in the E. coli host deposited under the Budapest Treaty with DSMZ under accession number DSM 15334; c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence which has at least 70% identity with the sequence shown from position 68 to 1417 in SEQ ID NO:3; and d) a fragment of (a), (b) or (c) that has alpha-amylase activity.
Owner:NOVOZYMES AS
Alpha-amylase variants
Owner:NOVOZYMES AS
Macrocyclic polyphenol compound, and preparation and application thereof in pharmacy
ActiveCN103641871APrevent proliferationInhibits alpha-amylaseOrganic active ingredientsSugar derivativesSide effectAmylosucrase activity
The invention provides a new use of a macrocyclic polyphenol compound separated from a penthorum chinense pursh extract in pharmacy. The macrocyclic polyphenol compound comprises a compound 1, a compound 2, a compound 3 and a compound 4, wherein the structural formulas of the compounds are as shown in the specification; the compounds have the following pharmacological activities that (1) the alpha-amylase activity is significantly inhibited, and the compounds have certain hypoglycemic effects, and (2) TGF (transforming growth factor)-beta1-induced hepatic stellate cell proliferation is significantly inhibited, and the compounds have anti-liver-fibrosis effects by pharmacology and efficacy experiments. The invention provides a novel medicament for preventing and treating diabetes mellitus, and an anti-liver fibrosis medicament. The medicament is abundant in raw material source, small in side effect, and beneficial to environmental protection, and has a relatively great clinical application value.
Owner:SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
Method
InactiveUS20130171296A1Improved animal performanceReduce amountPolypeptide with localisation/targeting motifMilk preparationAmylosucrase activityBuffer solution
The present invention relates to a method for identifying a pepsin resistant alpha amylase enzyme for use in a feed supplement comprising: i) providing an alpha amylase enzyme; ii) admixing said alpha amylase with corn based feed and buffer solution comprising a pepsin concentration of 9000 U / ml at pH 3, 40° C., 500 rpm for at least 120 minutes and analysing alpha amylase activity on said alpha amylase compared to a control sample; wherein said control sample differs in that no pepsin is present during incubation; and iii) selecting an alpha amylase enzyme which substantially maintains alpha amylase activity under the assay conditions; feed supplements and feedstuffs comprising a pepsin resistant alpha amylase and the use of pepsin resistant alpha amylases in feed.
Owner:DUPONT NUTRITION BIOSCIENCES APS
Processes for Producing a Fermentation Product
The present invention relates to processes for producing a fermentation product comprising (a) saccharifying starch-containing material below the initial gelatinization temperature in the presence of i) from 0.001-50 AGU / g DS, preferably 0.01 to 10 AGU / g DS alpha-glucosidase activity more than the native amount of endogenous alpha-glucosidase present in the starch-containing material, and ii) from 0 (zero) to 10 FAU-F / g DS of alpha-amylase activity, and (b) fermenting using a fermenting organism. The invention also relates to an enzymatic composition for use in a process of the invention.
Owner:NOVOZYMES NORTH AMERICA INC
Alpha-amylase mutants
InactiveUS7115409B1Improve stabilitySpecific activitySugar derivativesDetergent compounding agentsMutantAlpha-amylase activity
The present invention relates to a method of constructing a variant of a parent Termamyl-like alpha-amylase, which variant has alpha-amylase activity and at least one altered property as compared to the parent alpha-amylase, comprises i) analysing the structure of the parent Termamyl-like alpha-amylase to identify at least one amino acid residue or at least one structural part of the Termamyl-like alpha-amylase structure, which amino acid residue or structural part is believed to be of relevance for altering the property of the parent Termamyl-like alpha-amylase (as evaluated on the basis of structural or functional considerations), ii) constructing a Termamyl-like alpha-amylase variant, which as compared to the parent Termamyl-like alpha-amylase, has been modified in the amino acid residue or structural part identified in i) so as to alter the property, and, optionally, iii) testing the resulting Termamyl-like alpha-amylase variant with respect to the property in question.
Owner:NOVOZYMES AS
High efficiency preparation method of high temperature alpha-amylase and mutant thereof
ActiveCN101457230AImprove translationEfficient secretionBacteriaMicroorganism based processesBacillus licheniformisAmylosucrase activity
A high temperature alpha-amylase and a high efficiency preparation method of the mutant thereof belongs to the fermentation engineering field. The invention provides a method for high efficiently secreting expressing a high temperature alpha-amylase and mutant thereof with alpha-amylase activity through a bacillus, especially a bacillus licheniformis mutant strain as a host cell. The synthesis and secretion level of the high temperature alpha-amylase or mutant thereof of the recombinant strain of the invention reaches 18-25mg / mL during fed-batch fermentation and is 3-4.5 times of the original strain high temperature alpha-amylase synthesis level. The high temperature alpha-amylase of the invention is useful for reducing the fermentation manufacture cost of the industrial enzyme, simplifying the fermentation manufacture process and reducing fermentation industrial environment pressure.
Owner:福建福大百特生物科技有限公司
Alpha-amylase mutants
InactiveUS20030170769A1Microbiological testing/measurementTissue cultureMutantAlpha-amylase activity
The present invention relates to a method of constructing a variant of a parent Termamyl-like alpha-amylase, which variant has alpha-amylase activity and at least one altered property as compared to the parent alpha-amylase, comprises i) analysing the structure of the parent Termamyl-like alpha-amylase to identify at least one amino acid residue or at least one structural part of the Termamyl-like alpha-amylase structure, which amino acid residue or structural part is believed to be of relevance for altering the property of the parent Termamyl-like alpha-amylase (as evaluated on the basis of structural or functional considerations), ii) constructing a Termamyl-like alpha-amylase variant, which as compared to the parent Termamyl-like alpha-amylase, has been modified in the amino acid residue or structural part identified in i) so as to alter the property, and, optionally, iii) testing the resulting Termamyl-like alpha-amylase variant with respect to the property in question.
Owner:NOVOZYMES AS
Enyzmes having alpha amylase activity and methods of making and using them
The invention relates to alpha amylases and to polynucleotides encoding the alpha amylases, and methods of making and using them. In addition methods of designing new alpha amylases and methods of use thereof are also provided. The alpha amylases have increased activity and stability at increased pH and temperature.
Owner:VERENIUM CORPORATION
Method and application for improving fermentation enzyme production of bacillus licheniformis by knocking out spoIIQ and pcf genes
ActiveCN110055204AImprove fermentation effectStable growthBacteriaStable introduction of DNABiotechnologyMicroorganism
The invention relates to a method and an application for improving fermentation enzyme production of bacillus licheniformis by knocking out spoIIQ and pcf genes. Recombinant (B. licheniformis) DL-1 delta spoIIQ delta pcf is acquired by knocking out the spoIIQ and pcf genes, spore forming rate is reduced to 0.16%, and recombinant bacterium alpha-amylase activity is improved by 10.9% in flask shaking fermentation culture and improved by 19.3% in 5L fermentation culture. According to the method, fermentation activity of target enzymes of the bacillus licheniformis is improved to some extent, strain fermentation production performances are improved, and ideals and method reference can be provided for genetic engineering breeding of industrial microorganisms.
Owner:QILU UNIV OF TECH
Alpha-amylase activity inhibitors
InactiveUS7037536B2Strong inhibitory activityBiocideMetabolism disorderDyslipidemiaAmylosucrase activity
The present invention relates to an α-amylase activity inhibitor, and to food and beverages comprising the α-amylase activity inhibitor. The α-amylase activity inhibitor of the present invention exhibits remarkably excellent α-amylase inhibitory activity as compared with guava extract. Accordingly, food and beverages containing the α-amylase activity inhibitor are diet food and beverages suitable for people of high blood-sugar level or hyderlipidemia.
Owner:YAKULT HONSHA KK
Preparing purification method for thermophilic alpha-amylase
InactiveCN101063115AImprove purification efficiencyLow costEnzymesFermentationAlkalinityPurification methods
The invention discloses a preparing method of thermophilic alpha-amylase (PFA), which comprises the following steps: heating and dissolving recombinant protein inclusion body under the condition of alkalinity; diluting; compositing; separating and purifying through column layer. This method possesses high reclaiming ratio and high alpha-amylase activity.
Owner:SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
Fungal alpha-amylase variant with high maltose generation rate and preparation method for fungal alpha-amylase variant
ActiveCN106047844AIncreased maltose contentVector-based foreign material introductionGlycosylasesBiotechnologyGeneration rate
The invention discloses a fungal alpha-amylase variant with high maltose generation rate and a preparation method for the fungal alpha-amylase variant. The alpha-amylase variant is obtained by substituting one and / or multiple areas and / or positions of areas 77-81, areas 135-140, areas 214-220 and areas 331-335 correspondingly shown as SEQ ID NO:2, and has alpha-amylase activity. Compared with parental fungal alpha-amylase, the fungal alpha-amylase variant with high maltose generation rate has the advantages that the maltose content of a starch hydrolysate of the fungal alpha-amylase variant is increased by about 5 percent, and the fungal alpha-amylase variant has application advantages in industrial production of high-maltose syrup.
Owner:合肥停弦渡生物科技有限公司
Polypeptides having alpha amylase activity
InactiveUS20160083703A1Improve washing effectMolecular stabilizationAnimal cellsBacteriaAmylaseAmylosucrase activity
The present invention relates to polypeptides having alpha-amylase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Owner:NOVOZYMES AS
Rhizomucor miehei alpha-amylase, and encoding gene and application of rhizomucor miehei alpha-amylase
ActiveCN108165540AIncrease specific volumeDelay agingFungiPre-baking dough treatmentPichia pastorisReaction temperature
The invention relates to rhizomucor miehei alpha-amylase, and an encoding gene and an application of the rhizomucor miehei alpha-amylase. A protein has alpha-amylase activity; the gene of the proteinis efficiently expressed in pichia pastoris; the maximum enzyme activity after high density fermentation of the pichia pastoris is 29794.2U / mL; the specific enzyme activity after purification is 3502.1U / mg; an optimum reaction pH (potential of hydrogen) is 6.0; the protein keeps stable when the pH is 4.5-8.0; an optimum reaction temperature is 75 DEG C; and the protein keeps the higher enzymic activity below 65 DEG C and has better thermal resistance. The amylase can increase a specific volume of steamed bread by 7.7% and retard aging of the steamed bread when the amylase is applied to the steamed bread; and a maltose content can reach 54.1% when the amylase is applied to maltose production. The amylase has good utilization potentiality in the food industry.
Owner:CHINA AGRI UNIV
Polypeptides having alpha-amylase activity and polynucleotides encoding same
The present invention relates to isolated polypeptides having alpha-amylase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Owner:NOVOZYMES AS
Method for producing oenological tannins and enzymatic composition
InactiveUS20030054056A1Improve impact on tasteBiocideTea extractionAmylosucrase activityExoxylanase activity
The invention concerns an enzymatic method for making oenological tannins starting with lumps of wood and an enzymatic method for transforming tannins into tannins for wine-making purposes. The inventive method comprises a step which consists in contacting the lumps of wood or tannins with an aqueous solution comprising a composition containing enzymes of the cellulase class. The invention also concerns an enzymatic composition mainly consisting of enzyme of the cellulase class for making oenological tannins, comprising an endocellulase activity, a xylanase activity a beta-mannanase and / or alpha-amylase activity.
Owner:ETAB ROBERT STIERNON
Polypeptides Having Alpha-Amylase Activity and Polynucleotides Encoding Same
The present invention relates to isolated polypeptides having alpha-amylase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.
Owner:NOVOZYMES AS
Cyclocarya paliurus leaf solid beverage, as well as preparation method and application thereof
ActiveCN105851764AHigh dissolution rateEnhanced inhibitory effectFood extractionSolubilityWater soluble polysaccharides
The invention provides a cyclocarya paliurus leaf solid beverage, as well as a preparation method and application thereof. The solid beverage is prepared from the following components in parts by weight: 1g-3g of cyclocarya paliurus water-soluble polysaccharides, 1g-3g of cyclocarya paliurus flavone, 0.1g-0.5g of cyclocarya paliurus triterpene, 95g-98g of cyclocarya paliurus leaf raw powder, 5g-60g of inulin and 5g-60g of wheat aleurone layer. The cyclocarya paliurus leaf solid beverage is high in active ingredient (including cyclocarya paliurus polysaccharides), solubility, is beneficial to small intestine absorption of effective ingredients, and can be used for remarkably improving the bioavailability of the preparation. The ingredients of the cyclocarya paliurus leaf solid beverage can be well synergized, and has an alpha-amylase activity inhibition effect remarkably superior to that of conventional cyclocarya paliurus tea.
Owner:NANJING FORESTRY UNIV
Method of improving alpha-amylase activity in bacillus subtilis fermentation liquid
ActiveCN106754605AImprove α-amylase activityBacteriaMicroorganism based processesEnzyme digestionGene engineering
The invention belongs to the technical field of biological fermentation engineering and particularly relates to a method of improving alpha-amylase activity in bacillus subtilis fermentation liquid. A bacillus subtilis strain genome is modified by a gene engineering method, and the method particularly comprises the steps of PCR clonal expansion of sdpBC partial sequence DNA, enzyme digestion and connection, conversion, screening, identification, conversion from homologous single exchange recombinant plasmid to bacillus subtilis 168, detection and identification. According to the method, a bacillus subtilis 168 strain having a relatively clear physiology and conversion background and completing sequencing is taken as a research example, and a sporulation delay protein gene sdpC is subjected to insertion mutation by a homologous recombination method. A preliminary study shows that the viable count and the alpha-amylase activity of the modified strain obtained by the method are better increased and improved, the strain has a better application value, and an application foundation is laid for modification of other strains.
Owner:河南崤函生物科技有限公司
Combined amylase and application thereof
ActiveCN110628747AImprove starch digestibilityIncrease feed intakeMicroorganism based processesAccessory food factorsGlucoamylase activityGeneration rate
The invention discloses combined amylase and an application thereof, the combined amylase comprises medium-temperature alpha-amylase, pullulanase and glucoamylase, and the ratio of pullulanase activity (u) to medium-temperature alpha-amylase activity (u) to glucoamylase activity (u) is 1: 5-10: 50-80; wherein a fermentation strain of the medium-temperature alpha-amylase is bacillus subtilis, the fermentation strain of pullulanase is bacillus subtilis, and the fermentation strain of glucoamylase is aspergillus niger. According to the combined amylase provided by the invention, the feed intake of piglets is increased and the growth performance of the piglets is improved by regulating and controlling the reducing sugar generation rate, the digestibility of starch in a feed can be effectivelyimproved by adding the combined amylase, the feed intake of the piglets is remarkably increased, and the combined amylase has a wide application prospect.
Owner:GUANGDONG VTR BIO TECH
Preparation method and application of Southeast Asia herbal medicine combination extract
ActiveCN105106317ALong application timeHigh economic valueMetabolism disorderAntinoxious agentsSoutheast asiaAmylosucrase activity
The invention discloses a preparation method of Southeast Asia herbal medicine combination (cassia auriculata, ficus deltoidea and orthosiphon aristatus) extract, and application thereof in inhibiting alpha-amylase activity and antioxidant activity. Active ingredients are medium-polarity active ingredients obtained by activity screening of inhibiting alpha-amylase activity and antioxidant activity of cassia auriculata, ficus deltoidea and orthosiphon aristatus extracts. The preparation process is environment-friendly and safe, steps are simple and unique, and the obtained extract is good in stability and lasting in effect and can be used for adjuvant therapy of diabetes as well as resistance to inflammation and aging.
Owner:李文骥
Polypeptides having alpha amylase activity
InactiveUS20160083704A1Improve washing effectMolecular stabilizationDetergent compounding agentsGlycosylasesAmylaseAmylosucrase activity
The present invention relates to polypeptides having alpha-amylaseactivity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Owner:NOVOZYMES AS
Berberine drug as well as preparation method and application thereof
ActiveCN106749228AOvercome solubilityOvercome the disadvantage of low bioavailabilityOrganic active ingredientsOrganic chemistryBerberineSolubility
The invention relates to the technical field of medicine, in particular to a novel berberine drug as well as a preparation method and an application thereof in preparation of drugs for treating diabetes. The chemical structural formula of the compound is shown in formula I, and the compound overcomes the defects of poor solubility and low bioavailability of berberine. An alpha-amylase activity inhibition experiment on the compound verifies that the compound can be used for treating diabetes.
Owner:上海维京生物医药科技有限公司
Alpha amylases in feed
InactiveUS20170247673A1Reduce amountImprove performancePolypeptide with localisation/targeting motifFood processingAssayAmylosucrase activity
The present invention relates to a method for identifying a pepsin resistant alpha amylase enzyme for use in a feed supplement comprising: i) providing an alpha amylase enzyme; ii) admixing said alpha amylase with com based feed and buffer solution comprising a pepsin concentration of 9000 U / ml at pH 3, 40° C., 500 rpm for al least 120 minutes and analysing alpha amylase activity on said alpha amylase compared to a control sample; wherein said control sample differs in that no pepsin is present during incubation; and iii) selecting an alpha amylase enzyme which substantially maintains alpha amylase activity under the assay conditions; feed supplements and feed stuffs comprising a pepsin resistant alpha amylase and the use of pepsin resistant alpha amylases in feed.
Owner:DUPONT NUTRITION BIOSCIENCES APS
The application of the extract of C. chinensis in the preparation of hypoglycemic drugs
InactiveCN103638073BEasy to prepareGood hypoglycemic activityMetabolism disorderPlant ingredientsAcute toxicity testingMedicine
The invention discloses an application of penthorum Chinese pursh extract in the preparation of hypoglycemic drugs. The penthorum Chinese pursh extract is subjected to the following experiments: (1) in-vitro alpha-amylase activity inhibition experiments, wherein the experiment results show that the penthorum Chinese pursh extract has a prominent inhibiting effect on alpha-amylase; (2) experiment of penthorum Chinese pursh extract's effect on mouse starch tolerance, wherein the experiment results show that the penthorum Chinese pursh extract can prominently reduce the blood sugar concentration after the mice eat the starch; (3) acute toxicity experiment of penthorum Chinese pursh extract on mice through intragastric administration, wherein the experiment results show that the penthorum Chinese pursh extract has no prominent side or toxic effect on mice when reducing the blood sugar level. So the invention provides a novel way for preventing and treating diabetes in clinic. The penthorum Chinese pursh extract has a simple preparation method and a great application value in clinic.
Owner:SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
Method for detecting alpha-amylase activity by soluble starch resonance scattering spectrometry
InactiveCN101320000AEasy to operateLow costMaterial analysis by observing effect on chemical indicatorScattering properties measurementsAlpha-amylase activityUltimate tensile strength
The invention discloses a soluble starch resonance scattering spectra method for detecting Alpha-amylase activity. The resonance scattering strength I423nm of Alpha-amylase with known enzyme activity and the resonance scattering strength I0 of reagent blank are utilized for working out Delta I423nm which is equal to the difference between I423nm and I0 and is used as a standard curve. Then, Delta I of Alpha-amylase with unknown concentration is determined. The concentration of Alpha-amylase of an object to be determined is worked out according to a working curve. The soluble starch resonance scattering spectra method has the advantages of simple equipment, convenient operation, easily obtained reagent and low cost. And the method can be finished just through a spectrophotometer. The soluble starch of substrates is nontoxic.
Owner:GUANGXI NORMAL UNIV
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