High efficiency preparation method of high temperature alpha-amylase and mutant thereof

A technology for mutants and amylases, applied in the field of high-efficiency preparation of high-temperature α-amylases and their mutants
CN101457230AActive Publication Date: 2009-06-17福建福大百特生物科技有限公司
2 Cites 10 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
福建福大百特生物科技有限公司
Publication Date
2009-06-17

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

A high temperature alpha-amylase and a high efficiency preparation method of the mutant thereof belongs to the fermentation engineering field. The invention provides a method for high efficiently secreting expressing a high temperature alpha-amylase and mutant thereof with alpha-amylase activity through a bacillus, especially a bacillus licheniformis mutant strain as a host cell. The synthesis and secretion level of the high temperature alpha-amylase or mutant thereof of the recombinant strain of the invention reaches 18-25mg / mL during fed-batch fermentation and is 3-4.5 times of the original strain high temperature alpha-amylase synthesis level. The high temperature alpha-amylase of the invention is useful for reducing the fermentation manufacture cost of the industrial enzyme, simplifying the fermentation manufacture process and reducing fermentation industrial environment pressure.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention is a method for efficiently preparing high-temperature alpha-amylase with starch hydrolysis activity and mutants thereof by using bacillus host cells. The high-temperature α-amylase with starch hydrolysis activity in the present invention is derived from the bacterial strain of Bacillus licheniformis, and the mutant of the high-temperature α-amylase is derived from the artificial mutant of α-amylase of the bacterial strain of Bacillus licheniformis. Further, the present invention uses a specific expression vector to efficiently secrete, express and prepare high-temperature α-amylase and its mutants in Bacillus, especially Bacillus licheniformis host cells. The invention belongs to the field of fermentation engineering. Background technique

[0002] Starch is a class of polymer substances formed by the polymerization of glucose. Starch consists of two parts, amylose and amylopectin. Starch granules are generally round or irregular, with...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More