Alpha-amylase mutants

a technology of alpha-amylase and mutants, which is applied in the field of alpha-amylase mutants, can solve the problems of difficult to obtain .alpha-amylase crystals suitable for solving structure problems, and it is difficult to determine the three-dimensional structure of all alpha-amylases, so as to improve specific activity, increase stability, and improve stability
US20030170769A1Inactive Publication Date: 2003-09-11NOVOZYMES AS
0 Cites 10 Cited by

Patent Information

Authority / Receiving Office
US · United States
Patent Type
Applications(United States)
Current Assignee / Owner
NOVOZYMES AS
Publication Date
2003-09-11
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The present invention relates to a method of constructing a variant of a parent Termamyl-like alpha-amylase, which variant has alpha-amylase activity and at least one altered property as compared to the parent alpha-amylase, comprises i) analysing the structure of the parent Termamyl-like alpha-amylase to identify at least one amino acid residue or at least one structural part of the Termamyl-like alpha-amylase structure, which amino acid residue or structural part is believed to be of relevance for altering the property of the parent Termamyl-like alpha-amylase (as evaluated on the basis of structural or functional considerations), ii) constructing a Termamyl-like alpha-amylase variant, which as compared to the parent Termamyl-like alpha-amylase, has been modified in the amino acid residue or structural part identified in i) so as to alter the property, and, optionally, iii) testing the resulting Termamyl-like alpha-amylase variant with respect to the property in question.
Need to check novelty before this filing date? Find Prior Art

Description

[0001] This application is a continuation of U.S. application Ser. No. 09 / 636,252, filed on Aug. 10, 2000, which is a continuation of 09 / 327,563, filed on Jun. 8, 1999, which is continuation of Ser. No. 08 / 683,838, filed on Jul. 18,1996, now U.S. Pat. No. 6,022,724, which is a continuation-in-part of Ser. No. 08 / 600,908, filed on Feb. 13, 1996, now U.S. Pat. No. 5,989,169, which is a 371 of PCT / DK96 / 00057, filed on Feb. 5, 1996, and claims priority under 35 U.S.C. 119 of Danish applications 0128 / 95, filed on Feb. 3, 1995, 1192 / 95, filed on Oct. 23, 1995, and 1256 / 95, filed on Nov. 10, 1995, the contents of which are fully incorporated herein by reference.

[0002] The present invention relates to a novel method of designing .alpha.-amylase mutants with predetermined properties, which method is based on the hitherto unknown three-dimensional structure of bacterial .alpha.-amylases.

[0003] .alpha.-Amylases (.alpha.-1,4 glucan-4-glucanohydrolase, EC 3.2.1.1) constitute a group of enzymes w...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More