The invention belongs to the technical field of
maltose, and particularly relates to a preparation method and application of
maltose syrup. The preparation method of the malt syrup comprises the following steps: S1, adding water into
corn starch to obtain a mixture, and extruding to obtain a substance I; s2, adding water into the substance I to regulate the
mass fraction of the substance I to be 25-35% and the pH value to be 5.5-6.5, adding high-temperature-resistant alpha-
amylase and
anhydrous calcium chloride, liquefying at 90-100 DEG C for 20-30 minutes, cooling to 65-75 DEG C, adding medium-temperature alpha-
amylase, and continuing liquefying for 15-20 minutes to obtain a substance II; s3, feeding the substance II into a saccharifying tank, adjusting the pH value to 5.0-5.5 and the temperature to 50-60 DEG C, adding modified beta-
amylase,
isoamylase and
sodium thiosulfate, saccharifying for 3-6 hours, heating to 55-65 DEG C, adding
pullulanase, and saccharifying for 5-10 hours to obtain a substance III; and S4, carrying out
enzyme deactivation treatment on the substance III, carrying out secondary
filtration and decoloration, carrying out
ion exchange, collecting an
ion exchange liquid, and carrying out vacuum concentration. The preparation method of the malt syrup is simple to operate, and the prepared malt syrup is high in
maltose content and good in stability.