Trehalose synthase of streptomyces griseochromogenes and coding gene and application of trehalose synthase

Abstract

The invention discloses trehalose synthase of streptomyces griseochromogenes and a coding gene and application of the trehalose synthase. The trehalose synthase gene is cloned from the streptomyces griseochromogenes. The trehalose synthase can convert maltose to generate trehalose, the proper reaction temperature of the trehalose synthase is 15-35 DEG C and is 20-25 DEG C preferably, and the proper pH (potential of hydrogen) of the trehalose synthase ranges from 6.0 to 8.0 and ranges from 7.0 to 7.5 preferably. The conversion efficiency of the trehalose synthase is high, the maltose is used as a substrate, conversion rate of the maltose is about 80% at the reaction temperature of 25 DEG C, and less than 5% of glucose is generated to be beneficial to increase of the conversion rate of the substrate. The trehalose synthase can be used for converting commercial ultrahigh malt syrup to prepare the trehalose, and a novel method using an enzymic method to produce the trehalose industrially can be provided.

Description

technical field [0001] The invention belongs to the technical fields of genetic engineering and modern enzyme engineering, and specifically relates to a trehalose synthase from Streptomyces grisea chromogenes, its coding gene and application. Background technique [0002] Trehalose (Trehalose) is a non-reducing disaccharide composed of two molecules of glucose connected by α,α-1,1 glycosidic bonds. It is a safe and reliable natural sugar. It was introduced from rye by Wiggers in 1832. It was extracted for the first time from ergot fungus, and subsequent research found that trehalose exists widely in many edible animals, plants and microorganisms in nature, such as mushrooms, seaweed, beans, shrimp, bread, beer that people eat in daily life Trehalose is high in trehalose and yeast fermented foods. [0003] Trehalose has a magical protective effect on organisms, because trehalose can form a unique protective film on the cell surface under harsh environmental conditions such a...

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Problems solved by technology

[0013] At present, many literatures and patents have reported the cloning and isolation of trehalose synthesis-related genes, and the application of these trehalose synthase genes in the preparation of trehalose, but the synthesis pathways of these trehalose synthesis-related enzymes are different, or the bacterial species Different sources lead to very different DNA sequences and amino acid sequences, as well as different molecular weights, enzymatic properties, and conversion rates of enzymes to substrates, and there are not many genes and patents that can be used in industrial applications.

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