2 results about "Streptomyces phaeochromogenus" patented technology

This invention discloses an engineered bacterium producing tetracrine, its construction, and its application. The invention utilizes a strong promoter to drive the high expression of regulatory genes ovmZn and ovmWn in *Streptomyces neyagawa*, constructing a recombinant engineered strain Sne / pZWn; alternatively, it utilizes a strong promoter to drive the high expression of homologous genes of both, including the regulatory genes ovmZ and ovmW from *Streptomyces chromogenicus*, in *Streptomyces neyagawa*, constructing a recombinant engineered strain Sne / pZW. These engineered strains can express previously silenced gene clusters, activating tetracrine biosynthesis and laying the foundation for the development and application of tetracrine.

The invention discloses a preparation method of avilamycin fermentation liquor, and belongs to the technical field of microbial fermentation. According to the method, streptomyces viridochromogenes is used as a strain, a fermentation production culture medium with a specific formula is adopted for fermentation, the fermentation process is precisely controlled through feeding by a flow addition method, and finally the avilamycin fermentation liquor is prepared. By optimizing a culture medium formula and a fermentation process, the fermentation titer of avilamycin reaches more than 6500u / ml, the fermentation time is controlled within 200h, the problems of low fermentation yield and high energy consumption in the prior art are effectively solved, and the method is suitable for industrial production.