58 results about "Streptomyces phaeochromogenus" patented technology

The invention relates to a liquid composite biological multi-control fertilizer. The invention is characterized in that the liquid composite biological multi-control fertilizer is prepared from the following raw materials in parts by weight: 1-3 parts of Streptomyces hygroscopicus solution, 1-3 parts of Streptomyces cinnamofuscus solution, 2-4 parts of Bacillus amyloliquefaciens solution, 2-4 parts of bacillus sphaericus solution, 7-15 parts of Bacillus mucilaginosus solution, 1-3 parts of Azotobacter chroococcum solution, 1-3 parts of Streptomyces aureochrogmoenes, 8-10 parts of Trichoderma viride fermentative degradation solution, 8-10 parts of matrix and 45-65 parts of sterilized water. The invention prepares a crop-absorbing carbohydrate and organic matter Trichoderma viride fermentative degradation solution, and provides high-efficiency beneficial microbial florae with phosphate solubilizing, potassium solubilizing, nitrogen fixation and disease resistance effects. The fertilizer can simultaneously perform the three functions of biological pesticide, biofertilizer and biological nutrients, and has the advantages of no toxicity or harm and no environment pollution. Compared with other fertilizers with the same nutrients, the crop yield is increased by 20%.

The invention discloses a solid composite biological multi-control water and moisture retention fertilizer and a preparing method and application thereof.The fertilizer is prepared from bentonite, matrix, streptomyces hygroscopicus liquid, bacillus megatherium liquid, bacillus amyloliquefaciens liquid, bacillus sphaericus liquid, bacillus mucilaginosus liquid, azotobacter chroococcum liquid, bacillus cereus liquid, bacillus subtilis liquid, golden streptomyces chromogenes liquid, streptomyces microflavus liquid and trichoderma viride liquid.The preparing method comprises the steps of 1, preparing bacterial liquid; 2, mixing and drying the different kinds of bacterial liquid and the matrix, and then mixing with bentonite for pelletizing.The fertilizer can achieve nitrogen fixation, dephosphorization and potassium release, absorb water to maintain soil moisture, promote soil organic matter decomposition, resist bacteria and insects, improve microbiota in soil and improve the structure of soil.

The invention discloses trehalose synthase of streptomyces griseochromogenes and a coding gene and application of the trehalose synthase. The trehalose synthase gene is cloned from the streptomyces griseochromogenes. The trehalose synthase can convert maltose to generate trehalose, the proper reaction temperature of the trehalose synthase is 15-35 DEG C and is 20-25 DEG C preferably, and the proper pH (potential of hydrogen) of the trehalose synthase ranges from 6.0 to 8.0 and ranges from 7.0 to 7.5 preferably. The conversion efficiency of the trehalose synthase is high, the maltose is used as a substrate, conversion rate of the maltose is about 80% at the reaction temperature of 25 DEG C, and less than 5% of glucose is generated to be beneficial to increase of the conversion rate of the substrate. The trehalose synthase can be used for converting commercial ultrahigh malt syrup to prepare the trehalose, and a novel method using an enzymic method to produce the trehalose industrially can be provided.

The invention provides streptomyces erythrochromogenes MO28, which has a preservation number of CGMCC No.3228. The strain is capable of inhibiting the growth of tomato gray mold. Results of panel antibacterial tests show that MO28 strain has high inhibition effect on various plant pathogenic fungi and plant pathogenic bacteria, wherein the fusarium moniliforme inhibition capacity is the highest and the fusarium moniliforme inhibition rate is up to 69.9 percent; and the pseudomonas syringae inhibition diameter is 53.7millimeter. The tomato fruit gray mold prevention and control efficiency of the MO28 is 96.56 percent and the tomato leaf gray mold prevention and control efficiency of the MO28 is 78.26 percent. The strain has no obvious difference from 28-percent chlorothalonil and diethofencarb wettable powder. Results of greenhouse prevention effect detection show that the efficiency of the prevention and control of gray mold on tomatoes, pepper, cucumbers, egg plants and strawberry of the MO28 is between 63 and 77 percent.

A marine streptomyces viridochromogenes strain for producing alkali-tolerant and salt-tolerant xylanase and application of the marine streptomyces viridochromogenes strain belong to the field of biotechnology. The strain is collected in the China General Microbiological Culture Collection Center, and the collection number is 5565. The strain can normally grow under the extreme alkaline environment of pH11, shows a good oligotrophy-tolerant characteristic, can degrade a variety of hemicellulose-containing biomasses including corn straws, rice straws, Jerusalem artichoke straws, kelp fibers andthe like, and has a broad application prospect in utilizing cheap biomass resources to produce bio-based chemicals. The strain can produce xylanase under optimum conditions, and the enzymatic activity of the xylanase can be as high as 68.9U / ml. The produced xylanase has the following properties: the optimum pH value is 6.0, the optimum temperature is 70 DEG C, the optimum substrate is beech xylan, and the produced xylanase does not have cellulase activity, has high pH stability and thermal stability, and can tolerate high-concentration NaCl. As an enzyme preparation coming from the ocean, thexylanase can be widely used in papermaking, food, feed, wine making, energy and other industries.

The invention discloses recombinant streptomyces ansochromogenes, which are streptomyces ansochromogenes having blocked sanP genes for biologically synthesizing nikkomycins. The recombinant streptomyces ansochromogenes are (Streptomyces ansochromogenes) TH322 sanPD CGMCC No.3086. The invention also provides a method for producing the nikkomycin Z and a culture medium of the nikkomycin Z. In the invention, the recombinant streptomyces ansochromogenes which only produce a single nikkomycin Z component are provided, the culture medium of the nikkomycin Z is optimized, and the recombinant streptomyces ansochromogenes can produce the single component nikkomycin Z in large scale with a yield which is 4.5 times that of the prior initial strain of streptomyces ansochromogenes TH322 and reduce production cost, and have a significant for the simplification of the separation and purification of products obtained after fermentation.

The present invention belongs to the field of antibiotic gene engineering, and is especially the process of cloning nicomycin biological synthetic gene sanU from one strain of Streptomyces ansochromogenes 7100 CGMCC 4.321; connecting the synthetic gene sanU with promoter of melanin biosynthetic structure gene, cloning onto the polycloning site of integrative single-copy plamid, transforming wild Streptomyces ansochromogenes 7100 CGMCC 4.321 protoplast to obtain nicomycin X component of the recombinant engineering bacterium in the yield 2 times higher than wild strain. The high-yield engineering bacterium may be used in preparing antiseptic medicines.

The present invention relates to a liquid compound biological preservative. The liquid compound biological preservative is characterized by consisting of the following raw materials in parts by weight: 5-25 parts of chitosan, 5-25 parts of epsilon-polylysine, 5-25 parts of lysozyme, 5-20 parts of streptomyces hygroscopicus liquid, 5-20 parts of streptomyces microflavus liquid, 5-20 parts of streptomyces aureochromogenes liquid, 5-20 parts of lactic streptococci liquid, 5-20 parts of bacillus subtilis liquid, 5-20 parts of bacillus licheniformis liquid, 5-20 parts of beer yeast liquid, 5-20 parts of paecilomyces lilacinus liquid and 5-20 parts of trichoderma harzianum liquid. The liquid compound biological preservative is high-efficient, non-toxic, healthy and nutritious, beneficial for food safety, and especially has significant effects on preservation of nanguo pears, actinidia arguta or strawberries.

The invention discloses a Streptomyces diastochromogenes, a fermentation product 5'-epi-SPA-6952A and an application thereof. The Streptomyces diastochromogenes of the invention has the preservation number of CGMCCNo.2281, the fermentation product thereof has favourable insecticidal activity, and the active ingredient with insecticidal activity in the fermentation product is 5'-epi-SPA-6952A. Thefermentation liquor and the active ingredient 5'-epi-SPA-6952A of the Streptomyces diastochromogenes of the invention both have favourable insecticidal activity and low production cost, is friendly to environment and has favourable application prospect. In addition, the compound 5'-epi-SPA-6952A is a new compound, thus having wide development and application prospect.

The invention relates to the technical field of microbiology, particularly to an application of a mixed formula preparation to control on nilaparvata lugens stal. Streptomyces diastatochromogenes is named streptomyces diastatochromogenes D. The strain is preserved in the CGMCC (China General Microbiological Culture Collection Center) of CCCCM(China Committee for Culture Collection of Microorganisms)on May 15th, 2007, and the preservation registration number is CGMCC No.2060. Metabolic products having an inhibition effect on nilaparvata lugens stal yeast-likesymbiotic bacteria can be obtained throughfermentation and extraction of the streptomyces diastatochromogenes D.The application is most significantly characterized in that the metabolic products of the streptomyces diastatochromogenes D and imidacloprid are mixed in certainproportion to prepare a new formula, the usage amount of a pesticide can be obviously reduced when the mixed formula is used for controlling the nilaparvata lugens stal, the control effect on the nilaparvata lugens stal can be effectively improved, and the development of the drug resistance of the nilaparvata lugens stal can be delayed.

A marine streptomyces viridochromogenes strain for producing alkali-tolerant and salt-tolerant xylanase and application of the marine streptomyces viridochromogenes strain belong to the field of biotechnology. The strain is collected in the China General Microbiological Culture Collection Center, and the collection number is 5565. The strain can normally grow under the extreme alkaline environment of pH11, shows a good oligotrophy-tolerant characteristic, can degrade a variety of hemicellulose-containing biomasses including corn straws, rice straws, Jerusalem artichoke straws, kelp fibers and the like, and has a broad application prospect in utilizing cheap biomass resources to produce bio-based chemicals. The strain can produce xylanase under optimum conditions, and the enzymatic activity of the xylanase can be as high as 68.9U / ml. The produced xylanase has the following properties: the optimum pH value is 6.0, the optimum temperature is 70 DEG C, the optimum substrate is beech xylan, and the produced xylanase does not have cellulase activity, has high pH stability and thermal stability, and can tolerate high-concentration NaCl. As an enzyme preparation coming from the ocean, the xylanase can be widely used in papermaking, food, feed, wine making, energy and other industries.

The present invention is one Streptomyces ansochromogenes gene blocking engineering bacterium to produce Nikemycin Z component. The present invention features that into the sanQ gene of one kind of wild strain of Streptomyces ansochromogenes, antiseptic resistance gene segment capable of expressing in Streptomyces ansochromogenes is inserted to prepare the gene blocking engineering bacterium. The SanQ gene is related with the synthsis of Nikemycin. HPLC analysis shows that, compared with wild version, the sanQ gene blocking engineering bacterium has the same capacity of producing Nikemycin Z component but cannot produc X component. The engineering bacterium has the same capacity of inhibiting agricultural pathogeneic fungi and body's pathogenetic fungi.

The invention discloses recombinant streptomyces ansochromogenes, a preparation method thereof and use thereof. The recombinant streptomyces ansochromogenes are recombinant bacteria obtained by introducing nikkomycin biologic synthesis gene clusters into the streptomyces ansochromogenes, wherein the nikkomycin biologic synthesis gene clusters can be introduced into the streptomyces ansochromogenes by recombinant plasmids pNIK. Compared with the streptomyces ansochromogene strain 7100 CGMCC4.321, the recombinant streptomyces ansochromogenes of the invention can improve the nikkomycin Z yield by about 1.8 times and the nikkomycin X yield by about 3 times. The recombinant streptomyces ansochromogenes obviously improve the yield of the nikkomycins and provides necessary technical preparation for the large-scale production of the nikkomycins.

The invention provides a compound continuous-cropping-resistant biological fungicide special for ginsengs / Chinese yams / peanuts and a preparation method thereof. The compound continuous-cropping-resistant biological fungicide is prepared from sheep manure, short and small bacillus pumilus, bacillus amyloliquefaciens liquid, bacillus sphaericus liquid, bacillus licheniformis liquid, paecilomyces fumosoroseus liquid, penicillium oxalicum liquid, streptomyces hygroscopicus liquid, streptomyces griseochromogenes liquid and an auxiliary matrix. The preparation method comprises the steps that 1, the bacterial liquids are prepared respectively and are mixed evenly; 2, main and auxiliary matrixes are evenly mixed, and then drying is performed under the pressure of 30-50 DEG C. The biological fungicide is resistant to continuous cropping, fixes nitrogen, dissolves phosphorus, releases potassium, absorbs moisture, keeps soil humidity, promotes decomposition of soil organic matter, is bacteriostatic and insect-resistant, improves micropopulation in soil, improves the functions of a soil structure, does not pollute the environment and is free of toxicity and residues and safe to human.

The invention provides a method for improving the output of nikkomycin. The invention specifically provides a fermentation medium for Streptomyces ansochromogenes and a method for preparing nikkomycin by using the medium. According to the invention, a proper amount of Tween 80 is added into the fermentation medium to further optimize the varieties and amounts of specific components of the fermentation medium and other culture conditions are controlled, so the output of nikkomycin is increased; the output of nikkomycin in the invention is as high as 42600 [mu]g / mL; and the method is simple, low in cost and suitable for industrial large-scale production.

The invention discloses a recombinant phospholipase D mutant and application thereof in synthesis of phosphatidylserine, and belongs to the technical field of bioengineering. Recombinant phospholipase is constructed firstly, wherein the recombinant phospholipase is obtained by removing signal peptide from the C end of PLD and adding a label MBP through connecting peptide on the basis of phospholipase from streptomyces spike chromogenes, and the recombinant phospholipase mutant is obtained by mutating one or more of amino acid at the 379 site, the 169 site and the 185 site of the recombinant phospholipase. The recombinant phospholipase mutant shows high trans-phosphatidylactivity, the conversion rate of phosphatidylcholine can reach 85.8%, the yield of phosphatidylserine can reach 72.12%, and the yield of phosphatidylserine can reach 57.69g / L. By means of the method, the production capacity of a unit catalyst and the reaction efficiency of the catalyst are improved, and the reaction cost is reduced.

The invention discloses a bacterial strain for increasing the yield of tetramycin Z and a method for preparing tetramycin Z by using the bacterial strain. The strain is classified and named as Streptomyces diastatochromogenes tz3-1 and is preserved on December 3, 2019, with an accession number being CGMCC No. 19069. The invention further discloses a preparation method of the streptomyces diastatochromogenes tz3-1. According to the streptomyces diastatochromogenes tz3-1 disclosed by the invention, the content of tetramycin Z in fermentation liquor obtained by fermenting the Streptomyces diastatochromogenes tz3-1 is 20.6 times higher compared with control Streptomyces diastatochromogenes D, so a foundation is laid for industrial production of tetramycin Z in the future.

The present invention provides streptomyces erythrochromogenes MO28, which has a preservation number of CGMCC No.3228. The strain is capable of inhibiting the growth of tomato gray mold. Results of panel antibacterial tests show that MO28 strain has high inhibition effect on various plant pathogenic fungi and plant pathogenic bacteria, wherein the fusarium moniliforme inhibition capacity is the highest and the fusarium moniliforme inhibition rate is up to 69.9 percent; and the pseudomonas syringae inhibition diameter is 53.7millimeter. The tomato fruit gray mold prevention and control efficiency of the MO28 is 96.56 percent and the tomato leaf gray mold prevention and control efficiency of the MO28 is 78.26 percent. The strain has no obvious difference from 28-percent chlorothalonil and diethofencarb wettable powder. Results of greenhouse prevention effect detection show that the efficiency of the prevention and control of gray mold on tomatoes, pepper, cucumbers, egg plants and strawberry of the MO28 is between 63 and 77 percent.

The invention discloses an frr expression reinforced recombined streptomyces diastatochromogenes, as well as a construction method and use. The recombined streptomyces diastatochromogenes excessively expresses ribosome cycle factor frr and has higher toyocamycin synthesis capability than streptomyces diastatochromogenes 1628. The construction process is as follows: 1) constructing an expression vector pIB139-frr; and 2) utilizing a conjugational transfer method to integrate the expression vector with the chromosome of the streptomyces diastatochromogenes so as to obtain the engineering bacteria. A promoter permoE* on the pIB139 vector is utilized to start expression of frr gene; the vector pIB139-frr is specifically integrated with the chromosome of the streptomyces diastatochromogenes 1628 by the conjugational transfer method so as to obtain engineering bacteria with genetic stability. Compared with the original strain, the engineering bacteria obviously improves the transcriptional level of the key enzyme gene toyF in the recombined toyocamycin synthesis route, increases the yield of the toyocamycin by at least 43.6%, and shortens the fermentation period by 12h.

The invention discloses a recombinant streptomyces diastatochromogenes with reinforced adpA expression, a construction method and an application. A transcriptional regulation factor adpA at a secondary metabolism network center is excessively expressed, and the recombinant streptomyces diastatochromogenes has higher toyocamycin synthesis capacity compared with streptomyces diastatochromogenes 1628. The construction method comprises the following construction processes: (1) constructing an expression vector pIB139-adpA; and 2) merging the expression vector into a chromosome of the streptomyces diastatochromogenes by a conjugal transfer method so as to obtain engineering bacteria. The expression of adpA gene is started by utilizing a promoter permE* on the pIB139 vector, and the vector pIB139-adpA is specifically merged into the chromosome of the streptomyces diastatochromogenes 1628 by the conjugal transfer method so as to obtain the engineering bacteria with genetic stability. Compared with the original strain, the recombinant streptomyces diastatochromogenes has the advantages that the transcriptional level of a key enzyme gene toyF in the synthetic route of recombinant bacteria toyocamycin is obviously strengthened, and the yield of toyocamycin is improved by at least 31.6%. A foundation is laid for further improving the yield of toyocamycin and realizing industrial production as soon as possible.

The invention relates to the field of microbes and provides a Streptomyces ansochromogenes fermentation medium and a method for preparing nikkomycin through the medium. Through use of a proper amount of threonine in the fermentation medium, further optimization of types and use amounts of components of the fermentation medium and control of other culture conditions, a nikkomycin yield is improved. The fermentation medium has a nikkomycin yield of 42800 micrograms per milliliter, has a simple formula and a low cost and is suitable for industrial large-scale production.

The present invention relates to the application of environmental microbe. Especially, by means of molecular technology, one new gene is cloned from Streptomyces ansochromogenes 7100 and the gene encodes one kind of 2-streptomycete dioxyenase. The encoded product has great value in biologically degrading industrial pollutant nitroalkane and in improving environment.

The invention discloses application of streptomyces diastatochromogenes metabolite in prevention and treatment of black spot and anthracnose of Zhejiang ophiopogon japonicus, and relates to the technical field of microorganisms. The strain disclosed by the invention is classified and named as Streptomyces diastatochromogenes, the serial number is tz3-1, the preservation date is December 3rd, 2019, and the preservation registration number is CGMCC No.19069. The most significant characteristic of the invention is that the liquid fermentation metabolite of the strain can inhibit the growth of Zhejiang ophiopogon japonicus anthracnose pathogenic bacteria Colletotrichum liriopes and black spot pathogenic bacteria Alternaria alternata hypha at the same time. Field trials show that the effect of the bacterial strain metabolite on prevention and treatment of black spot and anthracnose of Zhejiang ophiopogon japonicus is slightly better than that of conventional chemical pesticides.

The invention provides streptomyces diastatochromogenes with high yield of toyocamycin in genetic engineering as well as a construction method and application of the streptomyces diastatochromogenes. According to the method, a recombinant plasmid pIB139-RelA is constructed by a genetic engineering method, and is transferred into streptomyces diastatochromogenes 1628, the streptomyces diastatochromogenes with high yield of toyocamycin overexpresses a streptomyces coelicolor ppGpp synthetase RelA gene, and the overexpression product of the RelA gene can positively regulate and control the biosynthesis of toyocamycin, and is used for the production of toyocamycin. The yield of toyocamycin produced by fermentation of the strain obtained by the invention is at least increased to 1568mg / L, which is 10 times of the yield of the original strain, and a new technical support is provided for increasing the yield of toyocamycin in industrial production.

The invention discloses a gene cluster expression strengthened recombinant streptomyces diastatochromogenes and a construction method thereof. The gene cluster expression strengthened recombinant streptomyces diastatochromogenes excessively expresses a whole toy gene cluster for biosynthesizing toyocamycin and has stronger toyocamycin synthetic capability than streptomyces diastatochromogenes 1628. A construction process comprises the following steps: 1) constructing an expression vector pSET152-toyAM; and 2) integrating the expression vector into chromosome of the streptomyces diastatochromogenes by utilizing a conjugative transferring method to obtain an engineering bacterium. According to the construction method disclosed by the invention, specificity of the vector pSET152-toyAM is integrated into the chromosome of the streptomyces diastatochromogenes 1628 by utilizing the conjugative transferring method to obtain an engineering bacterium with genetic stability. Compared with an original strain, the gene cluster expression strengthened recombinant streptomyces diastatochromogenes has the advantage that the yield of toyocamycin is increased by 4.5 times.

The invention relates to a screening method for a high-yielding strain of polyoxin I and solves the technical problem of lower content of polyoxin I in an existing strain. An original strain is Streptomyces ansochromogenes; the method comprises steps as follows: a spore suspension of the original strain is prepared, a 5-fluorouracil-containing resistant plate is coated with the diluted spore suspension subjected to ultraviolet mutagenesis, and after culture, resistant strains are screened and the high-yielding strain of polyoxin I is obtained. Fermentation broth of the screened high-yielding strain of polyoxin I has an inhibition effect on alternaria alternata. The high-yielding strain can be rapidly screened out with the screening method.

The invention discloses a toyG expression-enhanced recombined streptomyces diastatochromogenes and a construction method and uses thereof. The recombined streptomyces diastatochromogenes excessively expresses that the key enzyme of biosynthetic toyocamycin-an adenosine succinic acid synthetase encoding gene toyG has a toyocamycin synthesis capacity higher than streptomyces diastatochromogenes 1628. The construction process is as follows: (1) constructing an expression vector pIB139-toyG; (2) utilizing a conjugational transfer method to integrate the expression vector into a chromosome of streptomyces diastatochromogenes to obtain engineering bacteria. A promoter permE on the vector pIB139 is utilized to open the expression of the toyG gene, and the conjugational transfer method is utilized to integrate the specificity of the vector pIB139-toyG into the chromosome of streptomyces diastatochromogenes 1628 to obtain the genetic-stability engineering bacteria. Compared with parent strains, the enzyme activity of recombinant strain adenosine succinic acid synthetase is increased by at least 1.2 times, and the yield of toyocamycin is increased by at least 27.1 percent.

The invention discloses a cellulose degrading bacterium and application thereof, and relates to a cellulose degrading microorganism and application thereof. According to the present invention, the cellulose degrading bacterium is Streptomyces ansochromogenes WF-10, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.23720; the invention also discloses an application of the cellulose degrading bacterium in cellulose biodegradation. The strain disclosed by the invention can grow on a CMC (Carboxymethyl Cellulose) plate taking sodium carboxymethyl cellulose as a unique carbon source, and an obvious cellulose decomposition transparent ring appears around a bacterial colony after the bacterial colony is dyed by Congo red; according to the invention, the cellulase can secrete four types of cellulase, including filter paper enzyme, endo-beta-1, 4-glucanase, exo-beta-1, 4-glucanase and beta-glucosidase; the capability of degrading natural cellulose is realized.

The invention discloses recombinant streptomyces ansochromogenes, a preparation method thereof and use thereof. The recombinant streptomyces ansochromogenes are recombinant bacteria obtained by introducing nikkomycin biologic synthesis gene clusters into the streptomyces ansochromogenes, wherein the nikkomycin biologic synthesis gene clusters can be introduced into the streptomyces ansochromogenes by recombinant plasmids pNIK. Compared with the streptomyces ansochromogene strain 7100 CGMCC4.321, the recombinant streptomyces ansochromogenes of the invention can improve the nikkomycin Z yield by about 1.8 times and the nikkomycin X yield by about 3 times. The recombinant streptomyces ansochromogenes obviously improve the yield of the nikkomycins and provides necessary technical preparation for the large-scale production of the nikkomycins.