The invention relates to the technical field of beverage processing, in particular to anti-fatigue functional flavor cold extraction coffee and a preparation method thereof, and the anti-fatigue functional flavor cold extraction coffee comprises the following raw materials: honey, red date juice, strawberry juice, banana juice, cube sugar, glucose, mixed coffee bean powder, skim milk powder and purified water. The cold extraction coffee is scientific and reasonable in raw material formula, unique in flavor and exquisite in preparation, has high economic benefits, social benefits and popularization value, is prepared according to a reasonable proportion, takes coffee beans and red date juice as main raw materials to produce the solid beverage, keeps the taste of coffee, has the taste of the red date juice, and enriches the drinking experience of the beverage. The red date juice is treated in an enzymolysis mode, nutrient elements in the red dates can be fully extracted, and the red date coffee can be drunk for a long time, meets the modern life requirements, is easy to absorb, safe and reliable, and is suitable for more people to drink by adding bananas and strawberries, so that the bitter taste of the coffee can be adjusted, the flavor of the beverage can be increased, and the product is suitable for more people to drink.