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Combined amylase and application thereof

An amylase and saccharification enzyme technology, applied in the application, enzyme, hydrolase and other directions, can solve problems such as affecting feed intake, and achieve the effects of increasing piglet feed intake, improving piglet production performance, and broad application prospects.

Active Publication Date: 2019-12-31
GUANGDONG VTR BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There is currently no preparation that can affect feed intake by regulating the rate of reducing sugar production in the digestive tract

Method used

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  • Combined amylase and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0028] A combined amylase, including 1ml of pullulanase, 5ml of medium-temperature α-amylase enzyme solution and 5ml of glucoamylase enzyme solution, the medium-temperature α-amylase and pullulanase fermentation strains are all Bacillus subtilis, fermented and cultivated The base is maltose 4%, glucose 1%, corn steep liquor 1%, ammonium sulfate 0.2%; Potassium hydrogen 0.3%, calcium chloride 0.02%.

Embodiment 2

[0030] A combined amylase, including 1ml of pullulanase, 5ml of medium-temperature α-amylase enzyme solution and 8ml of glucoamylase enzyme solution, the medium-temperature α-amylase and pullulanase fermentation strains are all Bacillus subtilis, fermented and cultivated The base is maltose 4%, glucose 1%, corn steep liquor 1%, ammonium sulfate 0.2%; Potassium hydrogen 0.3%, calcium chloride 0.02%.

Embodiment 3

[0032] A combined amylase, including 1ml of pullulanase, 10ml of medium-temperature α-amylase enzyme solution and 5ml of glucoamylase enzyme solution, the medium-temperature α-amylase and pullulanase fermentation strains are all Bacillus subtilis, fermented and cultivated The base is maltose 4%, glucose 1%, corn steep liquor 1%, ammonium sulfate 0.2%; Potassium hydrogen 0.3%, calcium chloride 0.02%.

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Abstract

The invention discloses combined amylase and an application thereof, the combined amylase comprises medium-temperature alpha-amylase, pullulanase and glucoamylase, and the ratio of pullulanase activity (u) to medium-temperature alpha-amylase activity (u) to glucoamylase activity (u) is 1: 5-10: 50-80; wherein a fermentation strain of the medium-temperature alpha-amylase is bacillus subtilis, the fermentation strain of pullulanase is bacillus subtilis, and the fermentation strain of glucoamylase is aspergillus niger. According to the combined amylase provided by the invention, the feed intake of piglets is increased and the growth performance of the piglets is improved by regulating and controlling the reducing sugar generation rate, the digestibility of starch in a feed can be effectivelyimproved by adding the combined amylase, the feed intake of the piglets is remarkably increased, and the combined amylase has a wide application prospect.

Description

technical field [0001] The invention relates to the technical field of enzyme preparations, in particular to a combined amylase and its application. Background technique [0002] Starch is the main source of energy for livestock and poultry, and more than 60% of the energy demand of the body comes from the digestion and utilization of starch. The digestibility of starch in the intestinal tract of animals is lower than expected. Noy ​​et al. showed that the digestibility of cornstarch in the terminal ileum of animals is less than 82% and does not increase with the age of animals. Adding exogenous amylase is a reliable way to improve the utilization rate of starch and improve the production performance of livestock and poultry. [0003] Starch in plant-based feed ingredients is composed of glucose connected by α-1,4-glycosidic bonds and α-1,6-glycosidic bonds, and the structure is distinguished between straight chains and branched chains. α-amylases target the α-1,4-glycosid...

Claims

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Application Information

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IPC IPC(8): C12N9/28C12N9/44C12N9/34A23K50/30A23K20/189A23K50/60C12R1/125C12R1/685
CPCC12N9/2417C12N9/2457C12N9/2428C12Y302/01001C12Y302/01041C12Y302/01003A23K50/30A23K20/189A23K50/60
Inventor 郭玉光杜红方杨博王敏陈书琴朱子昕陈思谢德欢刘凯丽冯国华周银华梁雪霞
Owner GUANGDONG VTR BIO TECH
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