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A kind of combined amylase and its application

A technology of amylase and glucoamylase, applied in the direction of application, enzyme, hydrolytic enzyme, etc., can solve the problems affecting feed intake, etc., and achieve the effect of increasing feed intake of piglets, improving piglet production performance, and reducing feed energy design standards

Active Publication Date: 2021-12-21
GUANGDONG VTR BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There is currently no preparation that can affect feed intake by regulating the rate of reducing sugar production in the digestive tract

Method used

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  • A kind of combined amylase and its application
  • A kind of combined amylase and its application
  • A kind of combined amylase and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A combined amylase, including 1ml of pullulanase, 5ml of medium-temperature α-amylase enzyme solution and 5ml of glucoamylase enzyme solution, the medium-temperature α-amylase and pullulanase fermentation strains are all Bacillus subtilis, fermented and cultivated The base is maltose 4%, glucose 1%, corn steep liquor 1%, ammonium sulfate 0.2%; Potassium hydrogen 0.3%, calcium chloride 0.02%.

Embodiment 2

[0030] A combined amylase, including 1ml of pullulanase, 5ml of medium-temperature α-amylase enzyme solution and 8ml of glucoamylase enzyme solution, the medium-temperature α-amylase and pullulanase fermentation strains are all Bacillus subtilis, fermented and cultivated The base is maltose 4%, glucose 1%, corn steep liquor 1%, ammonium sulfate 0.2%; Potassium hydrogen 0.3%, calcium chloride 0.02%.

Embodiment 3

[0032] A combined amylase, including 1ml of pullulanase, 10ml of medium-temperature α-amylase enzyme solution and 5ml of glucoamylase enzyme solution, the medium-temperature α-amylase and pullulanase fermentation strains are all Bacillus subtilis, fermented and cultivated The base is maltose 4%, glucose 1%, corn steep liquor 1%, ammonium sulfate 0.2%; Potassium hydrogen 0.3%, calcium chloride 0.02%.

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Abstract

The invention discloses a combined amylase and its application, which include active mesophilic α-amylase, pullulanase, and glucoamylase, wherein the pullulanase activity (u) and mesophilic α-amylase activity (u ) to glucoamylase activity (u) ratio is 1:5~10:50~80; wherein, the fermentation strain of mesophilic α-amylase is Bacillus subtilis, the fermentation strain of pullulanase is Bacillus subtilis, and the saccharification The fermentation strain of the enzyme is Aspergillus niger. The combination amylase provided by the invention can increase the feed intake of piglets and improve the growth performance of piglets by regulating the rate of reducing sugar production. Adding the combination amylase of the invention can effectively improve the digestibility of starch in the feed, significantly increase the feed intake of piglets, and has broad application potential. Application prospects.

Description

technical field [0001] The invention relates to the technical field of enzyme preparations, in particular to a combined amylase and its application. Background technique [0002] Starch is the main source of energy for livestock and poultry, and more than 60% of the energy demand of the body comes from the digestion and utilization of starch. The digestibility of starch in the intestinal tract of animals is lower than expected. Noy ​​et al. showed that the digestibility of cornstarch in the terminal ileum of animals is less than 82% and does not increase with the age of animals. Adding exogenous amylase is a reliable way to improve the utilization rate of starch and improve the production performance of livestock and poultry. [0003] Starch in plant-based feed ingredients is composed of glucose connected by α-1,4-glycosidic bonds and α-1,6-glycosidic bonds, and the structure is distinguished between straight chains and branched chains. α-amylases target the α-1,4-glycosid...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/28C12N9/44C12N9/34A23K50/30A23K20/189A23K50/60C12R1/125C12R1/685
CPCC12N9/2417C12N9/2457C12N9/2428C12Y302/01001C12Y302/01041C12Y302/01003A23K50/30A23K20/189A23K50/60
Inventor 郭玉光杜红方杨博王敏陈书琴朱子昕陈思谢德欢刘凯丽冯国华周银华梁雪霞
Owner GUANGDONG VTR BIO TECH
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