Salmonella characteristic chromogenic liquid nutrient medium, preparation method thereof and rapid detection method of salmonella
A technology of liquid culture medium and Salmonella, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, and microorganism measurement/inspection. It can solve the problems of inconvenient use of fluorometers, false negatives, etc., and achieve reduced manpower and strong specificity. , the effect of convenient operation
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Embodiment 1
[0028] Salmonella characteristic chromogenic liquid medium, the formula is: tryptone 0.8g, yeast powder 0.4g, sodium chloride 0.4g, lithium chloride 0.3g, sodium deoxycholate 0.1g, dipotassium hydrogen phosphate 0.1g, combination promoting Agent 0.02g, combined inhibitor 0.1g, characteristic enzymolysis substrate 0.02g, co-solvent 4mL, distilled water 100mL.
[0029] The preparation method of Salmonella characteristic chromogenic liquid medium: mix the above-mentioned tryptone, yeast powder, sodium chloride, lithium chloride, sodium deoxycholate, dipotassium hydrogen phosphate, combined accelerator, combined inhibitor, and distilled water in proportion , heat to dissolve, adjust the pH to 7.2 after cooling, sterilize at 115°C for 15 minutes, cool to room temperature, and use it as a mother liquor for later use; fully dissolve the characteristic enzymatic hydrolysis substrate in a co-solvent in proportion, filter and sterilize, add to the mother liquor, Shake well to become a l...
Embodiment 2
[0036] Salmonella characteristic chromogenic liquid medium, the formula is: tryptone 1.0g, yeast powder 0.6g, sodium chloride 0.6g, lithium chloride 0.4g, sodium deoxycholate 0.2g, dipotassium hydrogen phosphate 0.15g, combined to promote Agent 0.03g, combined inhibitor 0.15g, characteristic enzymatic hydrolysis substrate 0.03g, co-solvent 5mL, distilled water 100mL.
[0037] Wherein, the combination accelerator is sodium pyruvate and nicotinamide, weight ratio, sodium pyruvate: nicotinamide=1:2; combination inhibitor is sodium citrate and acarbose, weight ratio, sodium citrate: acarbose Sugar = 1:3, the characteristic enzymatic hydrolysis substrate is indole octyl ester, and the cosolvent is dimethyl sulfoxide.
[0038] The preparation method of Salmonella characteristic chromogenic liquid medium: mix the above-mentioned tryptone, yeast powder, sodium chloride, lithium chloride, sodium deoxycholate, dipotassium hydrogen phosphate, combined accelerator, combined inhibitor, and...
Embodiment 3
[0040] Salmonella characteristic chromogenic liquid medium, the formula is: tryptone 1.2g, yeast powder 0.8g, sodium chloride 0.8g, lithium chloride 0.5g, sodium deoxycholate 0.3g, dipotassium hydrogen phosphate 0.2g, combined to promote Agent 0.04g, combined inhibitor 0.2g, characteristic enzymatic substrate 0.04g, co-solvent 6mL, distilled water 100mL.
[0041] Wherein, the combination accelerator is sodium pyruvate and nicotinamide, weight ratio, sodium pyruvate: nicotinamide=1:3; combination inhibitor is sodium citrate and acarbose, weight ratio, sodium citrate: acarbose Sugar = 2:1, the characteristic enzymatic hydrolysis substrate is indole octyl ester, and the cosolvent is dimethyl sulfoxide.
[0042] The preparation method of Salmonella characteristic chromogenic liquid medium: mix the above-mentioned tryptone, yeast powder, sodium chloride, lithium chloride, sodium deoxycholate, dipotassium hydrogen phosphate, combined accelerator, combined inhibitor, and distilled wa...
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