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Salmonella characteristic chromogenic liquid nutrient medium, preparation method thereof and rapid detection method of salmonella

A technology of liquid culture medium and Salmonella, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, and microorganism measurement/inspection. It can solve the problems of inconvenient use of fluorometers, false negatives, etc., and achieve reduced manpower and strong specificity. , the effect of convenient operation

Active Publication Date: 2012-05-02
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, when the fluorescence intensity is weak, false negative results are prone to occur, and the use of a fluorometer also brings inconvenience to the experiment

Method used

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  • Salmonella characteristic chromogenic liquid nutrient medium, preparation method thereof and rapid detection method of salmonella

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Salmonella characteristic chromogenic liquid medium, the formula is: tryptone 0.8g, yeast powder 0.4g, sodium chloride 0.4g, lithium chloride 0.3g, sodium deoxycholate 0.1g, dipotassium hydrogen phosphate 0.1g, combination promoting Agent 0.02g, combined inhibitor 0.1g, characteristic enzymolysis substrate 0.02g, co-solvent 4mL, distilled water 100mL.

[0029] The preparation method of Salmonella characteristic chromogenic liquid medium: mix the above-mentioned tryptone, yeast powder, sodium chloride, lithium chloride, sodium deoxycholate, dipotassium hydrogen phosphate, combined accelerator, combined inhibitor, and distilled water in proportion , heat to dissolve, adjust the pH to 7.2 after cooling, sterilize at 115°C for 15 minutes, cool to room temperature, and use it as a mother liquor for later use; fully dissolve the characteristic enzymatic hydrolysis substrate in a co-solvent in proportion, filter and sterilize, add to the mother liquor, Shake well to become a l...

Embodiment 2

[0036] Salmonella characteristic chromogenic liquid medium, the formula is: tryptone 1.0g, yeast powder 0.6g, sodium chloride 0.6g, lithium chloride 0.4g, sodium deoxycholate 0.2g, dipotassium hydrogen phosphate 0.15g, combined to promote Agent 0.03g, combined inhibitor 0.15g, characteristic enzymatic hydrolysis substrate 0.03g, co-solvent 5mL, distilled water 100mL.

[0037] Wherein, the combination accelerator is sodium pyruvate and nicotinamide, weight ratio, sodium pyruvate: nicotinamide=1:2; combination inhibitor is sodium citrate and acarbose, weight ratio, sodium citrate: acarbose Sugar = 1:3, the characteristic enzymatic hydrolysis substrate is indole octyl ester, and the cosolvent is dimethyl sulfoxide.

[0038] The preparation method of Salmonella characteristic chromogenic liquid medium: mix the above-mentioned tryptone, yeast powder, sodium chloride, lithium chloride, sodium deoxycholate, dipotassium hydrogen phosphate, combined accelerator, combined inhibitor, and...

Embodiment 3

[0040] Salmonella characteristic chromogenic liquid medium, the formula is: tryptone 1.2g, yeast powder 0.8g, sodium chloride 0.8g, lithium chloride 0.5g, sodium deoxycholate 0.3g, dipotassium hydrogen phosphate 0.2g, combined to promote Agent 0.04g, combined inhibitor 0.2g, characteristic enzymatic substrate 0.04g, co-solvent 6mL, distilled water 100mL.

[0041] Wherein, the combination accelerator is sodium pyruvate and nicotinamide, weight ratio, sodium pyruvate: nicotinamide=1:3; combination inhibitor is sodium citrate and acarbose, weight ratio, sodium citrate: acarbose Sugar = 2:1, the characteristic enzymatic hydrolysis substrate is indole octyl ester, and the cosolvent is dimethyl sulfoxide.

[0042] The preparation method of Salmonella characteristic chromogenic liquid medium: mix the above-mentioned tryptone, yeast powder, sodium chloride, lithium chloride, sodium deoxycholate, dipotassium hydrogen phosphate, combined accelerator, combined inhibitor, and distilled wa...

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Abstract

The invention relates to the field of safety monitoring of food microorganisms, and discloses a salmonella characteristic chromogenic liquid nutrient medium, a preparation method thereof and a rapid detection method of salmonella. The salmonella characteristic chromogenic liquid nutrient medium comprises the following main components: tryptone, yeast powder, sodium chloride, lithium chloride, sodium deoxycholate, dipotassium phosphate, combined inhibitor, combined accelerator, characteristic enzymolysis substrate and cosolvent. The rapid detection method disclosed by the invention comprises two steps, i.e. pre-enrichment culture and chromogenic identification, and is characterized in that salmonella characteristic enzyme hydrolyzes corresponding substrates to result in that the culture medium is purple, thus rapidly judging the existence of salmonella; and the addition of the accelerator contributes to recovering damaged cells of salmonella and promoting growth of salmonella; and the added inhibitor can selectively inhibit the growth of other competitors so as to reduce the interference on detection by the competitors. The detection method disclosed by the invention has the advantages of short detection period, strong specificity and high accuracy, is simple to operate and is suitable for large-throughput detection of salmonella in food.

Description

technical field [0001] The invention relates to a Salmonella characteristic chromogenic liquid medium and a preparation method thereof, in particular to a Salmonella characteristic chromogenic liquid medium based on the principle of enzymolysis color development and a preparation method thereof. At the same time, the invention also relates to a rapid detection method for Salmonella, in particular to a method for rapid detection of Salmonella by using characteristic enzymes of Salmonella, which belongs to the field of safety monitoring of food-borne pathogenic bacteria. Background technique [0002] Salmonella is a Gram-negative non-bacillus. This kind of bacteria has a strong ability to survive in the external environment. It can survive in water, milk and meat for several months. The optimum temperature for its reproduction is 37°C. Common salmonella that cause food poisoning include Salmonella typhimurium, Salmonella choleraesuis, and Salmonella enteritidis. Salmonella ge...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/44C12Q1/04C12R1/42
Inventor 张巧艳周育徐俊锋
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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