Salmonella detection method and culture medium for detection

Abstract

The invention discloses a salmonella detection method, which carries out detection by using a capillary tube method. An MSTT culture medium is filled in a capillary tube, and per 1L of the culture medium, comprises the following components: 5.0-10.0 g of peptone, 0-5.0 g of a beef extract, 0-3.0 g of sodium chloride, 1.0-5.0 g of bile salts, 30.0-50.0 g of sodium thiosulfate, 9-10 mg of brilliant green, 4.0-5.0 g of iodine, 5.0-6.0 g of potassium iodide, 2.5-3.0 g of agar, 0 or 20-40 mg of novobiocin, and the balance of water. According to the invention, the capillary tube culture method and the MSTT culture medium are adopted for detecting salmonella, and salmonella pre-enrichment and enrichment steps are merged, so that a lot of time is saved, and the detection time is reduced by at least 24 h; and samples are subjected to preliminary screening by using a capillary tube culture method, so that a lot of subsequent separation and identification processes are saved.

Description

technical field [0001] The invention relates to a method for detecting Salmonella and a culture medium for detection, belonging to the field of microbial inspection. Background technique [0002] At present, the benchmark methods for the detection of Salmonella in foods such as the US FDA, AOAC, ISO and China National Standard all use pre-enrichment, enrichment, isolation and identification as the basic procedures, but there are slight differences in various media. But these benchmark methods are relatively time-consuming, manpower and material resources. In order to save manpower and time, researchers have developed many rapid detection methods. The principles are basically based on PCR and antigen-antibody reactions. They have played an important role in some fields, but they cannot replace the separation and culture methods in some aspects. The reason is that : 1. Regardless of PCR and antigen or antibody, it can only target limited target genes or antigens, and its cove...

AI Technical Summary

Problems solved by technology

However, these benchmark methods are relatively time-consuming, manpower and material resources

In order to save manpower and time, researchers have developed many rapid detection methods. The principles are basically based on PCR and antigen-antibody reactions. They have played an important role in some fields, but they cannot replace the separation and culture methods in some aspects. The reason is that : 1. Regardless of PCR and antigen-antibody, it can only target limited target genes or antigens, and its coverage of more than 2,000 serotypes of Salmonella is incomparable with the isolation and culture method, that is, the sensitivity is inferior to the isolation and culture method, so it cannot Shake the status of the isolation and culture method as the gold standard for Salmonella detection; 2. In some important projects, obtaining live bacteria is conclusive evidence; 3. When live bacteria are required for further analysis