Specific antibody of campylobacter jejuni specific multi-epitope artificial polypeptide and coated immunomagnetic beads and application thereof

A technology of Campylobacter jejuni and immune magnetic beads, applied in the direction of anti-bacterial immunoglobulin, application, peptide, etc., can solve the problem of small sampling volume, inability to distinguish live bacteria from dead bacteria, and the sensitivity and detection rate of traditional separation methods are reduced To achieve the effect of improving the detection rate, enrichment effect and efficiency, and improving sensitivity
CN102532283AInactive Publication Date: 2012-07-04ANIMAL AND PLANT & FOOD DETECTION CENTER JIANGSU ENTRY EXIT INSPECTION AND QUARANTINE BUREAU
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
ANIMAL AND PLANT & FOOD DETECTION CENTER JIANGSU ENTRY EXIT INSPECTION AND QUARANTINE BUREAU
Publication Date
2012-07-04
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a specific antibody of a campylobacter jejuni specific multi-epitope artificial polypeptide and coated immunomagnetic beads and an application thereof. The amino acid sequence of the artificial polypeptide prepared after tandem expression of a campylobacter jejuni specific surface protein multi-antigen epitope is SEQ ID No.1. A nucleotide sequence for encoding the campylobacter jejuni specific surface protein multi-antigen epitope is SEQ ID No.2. A kit for enriching campylobacter jejuni comprises immunomagnetic beads containing a specific antibody coated with the campylobacter jejuni specific multi-epitope artificial polypeptide. By applying the immunomagnetic beads or the kit, campylobacter jejuni can be enriched from a sample pre-enrichment liquid, and the sensitivity and detectable rate of subsequent separate culturing or PCR (Polymerase Chain Reaction) detection are increased greatly; and the specific antibody has the advantages of high specificity, high sensitivity, easiness, convenience, rapidness and suitability for large-scale sample detection.
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Description

Technical field

[0001] The invention belongs to the field of biological detection, and relates to a specific antibody of Campylobacter jejuni-specific multi-epitope artificial polypeptide and its coated immunomagnetic beads and applications. Background technique

[0002] Campylobacter jejuni (C.jejuni) exists in large numbers in the intestines of various wild or domestic animals. Contaminated water or food can cause an outbreak of diarrhea. Infected animals often have no obvious symptoms and carry it for a long time. bacteria. At present, C.jejuni infection has become one of the most common causes of acute gastroenteritis worldwide. In developing countries, it is an important cause of death from children’s diarrhea and the most common cause of traveler’s diarrhea. In the United States and other industrialized countries, diarrhea caused by Campylobacter jejuni infection is Salmonella, Shigella or E. coli (O157: H7) Diarrhea caused by 2 to 7 times. The bacteria can also cause Gu...

Claims

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