Salmonella choleraesuis immune colloidal gold rapid testing strip

A technology for detection of salmonella and test strips, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of cumbersome operation, long detection cycle, screening, etc., and achieve the effect of high detection specificity and sensitivity

Active Publication Date: 2014-07-23
UNIV OF SHANGHAI FOR SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the detection of Salmonella choleraesuis mainly relies on the biochemical identification stipulated in the national standard. The disadvantage is that the operation is cumbersome, the detection cycle is long, and it cannot adapt to the screening of a large number of samples.
Immunological detection is the fastest, accurate and stable rapid detection method that has appeared in recent years, but there is no rapid detection product that can be used in detection practice at home and abroad. This patent uses Salmonella choleraesuis as the detection target, and the technology claimed Immunocolloid gold rapid test strips involving Salmonella choleraesuis

Method used

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  • Salmonella choleraesuis immune colloidal gold rapid testing strip
  • Salmonella choleraesuis immune colloidal gold rapid testing strip
  • Salmonella choleraesuis immune colloidal gold rapid testing strip

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specific Embodiment approach

[0023] At first introduce the reagent and instrument used in the embodiment of the present invention:

[0024] main reagent

[0025] Freund's complete adjuvant and Freund's incomplete adjuvant, PEG, TWEEN-20Sigma; BSA was purchased from Shanghai Bright Biotechnology Co., Ltd.; colloidal gold solution was purchased from Shanghai Gold Standard Biotechnology Co., Ltd.; nitrocellulose membrane (NC membrane ) 135S Millipore; goat anti-mouse, HRP-goat anti-mouse, goat anti-rabbit were purchased from Shanghai Sangon Bioengineering Co., Ltd.; monoclonal antibody subtype identification kit was purchased from ZYMEDCat: 90-6550; Lot.60907259. The bacterial strains used in the cross-reaction experiment in the present invention can be obtained through commercial channels.

[0026] main instrument

[0027] Microplate reader SpectraMax M2 was purchased from Molecular Devices; Nanodrop2000C was purchased from Thermo Scientific; spotting device AD6010 was purchased from BIO-DOT; scanner Phan...

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Abstract

The invention discloses a salmonella choleraesuis immune colloidal gold rapid testing strip. The salmonella choleraesuis immune colloidal gold rapid testing strip comprises a sample pad, a colloidal gold pad, a NC membrane, and an absorbent pad which are connected successively; monoclonal antibody Mab05-D10, which is generated by a hybridoma cell strain with a preservation number of CGMCC No.7710, is used as gold-labeled antibody coupled colloidal gold, and is sprayed onto the colloidal gold pad; and monoclonal antibody Mab05-F10, which is generated by a hybridoma cell strain with a preservation number of CGMCC No.7712, is used as a detection antibody, and is sprayed onto the NC membrane so as to form detection lines. In addition, the invention also provides applications of the salmonella choleraesuis immune colloidal gold rapid testing strip in detecting food salmonella choleraesuis. It is shown by experiments that detection specificity and sensitivity of the salmonella choleraesuis immune colloidal gold rapid testing strip on salmonella choleraesuis are both relatively high.

Description

technical field [0001] The invention relates to an immune colloidal gold detection test strip, which belongs to the field of biological detection. Background technique [0002] Salmonella choleraesuis (Shigella boydii) belongs to Shigella group C and is an important food-borne pathogen that can enter the human body through contaminated meat, eggs, fish and other animal products, melon seeds, etc. , causing fever, abdominal pain, tenesmus, and sometimes manifested as systemic poisoning symptoms as toxic dysentery, which becomes chronic if the treatment is not complete. [0003] At present, the detection of Salmonella choleraesuis mainly relies on the biochemical identification stipulated in the national standard. The disadvantage is that the operation is cumbersome, the detection cycle is long, and it cannot adapt to the screening of a large number of samples. Immunological detection is the fastest, accurate and stable rapid detection method that has appeared in recent years...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/569G01N33/543
CPCG01N33/558G01N33/56916G01N2333/255
Inventor 刘箐刘程
Owner UNIV OF SHANGHAI FOR SCI & TECH
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