28 results about "Salmonella Be" patented technology

A specific protein fragment capable of representing salmonella as an antigen is obtained through screening and experimental verification, a mouse is immunized with the specific protein fragment as the antigen, and hybridoma cells capable of stably secreting monoclonal antibodies are obtained through a cell fusion technology. And finally, the anti-salmonella monoclonal antibody with high titer and good specificity is prepared. Similarly, the specific protein fragment is used for immunizing a rabbit to obtain the anti-salmonella polyclonal antibody. On the basis of a mouse monoclonal antibody and a rabbit polyclonal antibody, a salmonella colloidal gold immunochromatography detection method is established, the detection method is good in specificity, and a rapid, simple and convenient detection means is provided for detection of salmonella in food.

The invention discloses application of a method for enhancing the treatment effect of salmonella bacteriophage, and relates to the technical field of microorganisms. According to the method, bacteriophage is added into salmonella for in-vitro culture, after continuous subculture, the salmonella is dropwise added to an SS plate containing the bacteriophage, and resistant bacteria of the bacteriophage are screened. The split bacteriophage is screened aiming at the resistant bacteria of the bacteriophage, and the split bacteriophage and the initial bacteriophage form a bacteriophage cocktail formula, so that the salmonellosis can be well prevented and treated, and the generation of the resistant bacteria is delayed. The method has the beneficial effects that compared with the existing method for screening the bacteriophage-resistant bacteria through a double-layer plate method, the method is more convenient, the operation is simple, and the screening efficiency of the bacteriophage-resistant bacteria is greatly improved. The bacteriophage cocktail contains the bacteriophage for cracking the resistant bacteria, so that the treatment effect of the bacteriophage is improved, and the practical application scene is quite high.

The invention provides medical application of sanguinarine in preparation of a salmonella flagellum inhibitor, and belongs to the technical field of biological medicine. According to the invention, the flagellum of salmonella is used as a potential target for researching and developing the anti-salmonella toxicity drug, and a natural product capable of inhibiting the flagellum of salmonella is screened through a salmonella sliding motion inhibition test and a real-time fluorescent quantitative PCR test; in combination with tests such as a minimum inhibitory concentration determination test, a growth curve, inhibition of salmonella from adhesion to Caco-2 cells and the like, it is verified that sanguinarine can effectively inhibit the salmonella flagellum-mediated swimming ability on the basis of not influencing normal growth of bacteria, so that the pathogenic potential of salmonella is remarkably reduced, the effect of bacteriostasis instead of sterilization is achieved, and the application prospect is broad. A new research thought and a candidate natural compound are provided for prevention and treatment of salmonella infection, and potential practical application value is achieved.

To conduct detection of Salmonella, serotype transversely and specifically relative to other strains.SOLUTION: A heat-killed bacterial cell of Salmonella and antigen liquid containing an adjuvant are inoculated in a BALB / c mouse. After the inoculation, splenocytes or lymph node cells of the mouse and P3U1 are fused to prepare a hybridoma, and therefrom, a hybridoma for producing a monoclonal IgG antibody showing Salmonella specificity is selected, whereby three hybridomas for producing superior Salmonella specific antibodies are successfully acquired. Next, these monoclonal antibodies are used to detect Salmonella of a plurality serotypes and other strains according to sandwich ELISA, thereby obtaining a result in which Salmonella is detected serotype transversely, whereas other strains are not detected.SELECTED DRAWING: Figure 1

The present disclosure provides formulations, kits, and vaccines directed to immunization of animals against Salmonella. Methods of usng the formulations, kits, and vaccines for protection of avians against one or more species of Salmonella are also provided. For instance, the formulations, kits, and vaccines can induce an immune response in an animal against a Salmonella infection in which the Salmonella infection is caused by more than one Salmonella group bacterium (e.g., a Group B bacterium, a Group C bacterium, and a Group D bacterium).

The application belongs to the field of feed additives, and particularly discloses application of sodium butyrate in preparation of a feed additive against chicken white dysentery Salmonella, and sodium butyrate is added in chicken basic feed as the feed additive against chicken white dysentery Salmonella; the addition of sodium butyrate in chicken basic feed can significantly reduce the bacterial load of chicken white dysentery Salmonella infection in chicken liver and spleen, significantly increase the ratio of villus length to crypt depth of the cecum of chicken infected with chicken white dysentery Salmonella, and improve the intestinal absorption capacity; the content of CD3+ T cells, TCR+ T cells, macrophages and B cells in the liver of chicken infected with chicken white dysentery Salmonella can be significantly reduced; and then the body weight of chicken infected with chicken white dysentery Salmonella is significantly increased, and the survival rate of chicken infected with chicken white dysentery Salmonella is increased. In summary, the addition of sodium butyrate in chicken basic feed can enhance the resistance to chicken white dysentery Salmonella infection.

The invention relates to the technical field of microorganisms, in particular to avian salmonella and application thereof. The avian salmonella is avian salmonella enteritidis, and the preservation number of the avian salmonella enteritidis is CGMCC (China General Microbiological Culture Collection Center) No.25474. The avian salmonella provided by the invention is easy to proliferate and enrich, has good growth performance, biological safety and genetic stability, and can be used as a host bacterium for proliferation of multiple strains of avian salmonella bacteriophages to realize fermentation of multiple strains of bacteriophages by one strain of host bacterium, so that a high-titer preparation of multiple salmonella bacteriophages is prepared, and the application prospect is wide. The cost is effectively reduced while the production process is simplified.

The invention relates to the technical field of biological detection, and discloses a salmonella monoclonal antibody, a salmonella colloidal gold immunochromatography test strip based on the antibody and a preparation method of the salmonella colloidal gold immunochromatography test strip. Salmonella is used as an antigen, a polyclonal antibody and a monoclonal antibody are prepared respectively, the monoclonal antibody 8E71C8 is screened out, and the salmonella colloidal gold immunochromatography test strip is prepared. The purified monoclonal antibody is used as a 30 nm colloidal gold marker, the optimal pH and the optimal antibody labeling amount of binding of the monoclonal antibody and colloidal gold and the concentration of the polyclonal antibody used as a detection line are optimized, the prepared salmonella colloidal gold immunochromatography test strip is high in specificity, the sensitivity is about 1 * 10 < 4 > CFU / mL and is higher than the average detection lower limit of domestic similar products, and the salmonella colloidal gold immunochromatography test strip can be used for detecting salmonella. And the test strip is simple to operate and high in efficiency, can be used as a means for rapidly and visually detecting the salmonella, and has popularization and application values.

Provided are a method for preparing freezing shock-treated monocytes or macrophages loaded with attenuated Salmonella and the use thereof. The method comprises: loading engineering-modified attenuated Salmonella in monocytes or macrophages, and performed a freezing shock treatment on the monocytes or macrophages loaded with the strain by means of liquid nitrogen. The method for preparing the freezing shock-treated immune cells loaded with the strain is simple and easy to operate. The source problem of the monocytes or macrophages loaded with the engineering-modified attenuated Salmonella is solved, and the method has good application prospects. According to the strategy, by means of avoiding exposure and heterologous stimulation of bacteria, the biological safety of bacteria-based anti-tumor treatment is improved; and the anti-tumor immune response is promoted by means of the high titer of an intratumoral strain, so that a stronger anti-tumor efficacy is obtained.

To provide a novel treatment method for benign nervous system tumors, including schwannomas; and to provide a composition for use in the treatment method.SOLUTION: Provided herein are methods for a treating a subject having or at risk of having a benign nervous system tumor. The methods include administering to the subject a therapeutically effective amount of a composition comprising live attenuated Salmonella bacteria, optionally in combination with an immune checkpoint inhibitor and / or angiogenesis inhibitor. Preferably, the attenuated Salmonella is an attenuated strain of S. typhimurium, e.g., Salmonella enterica serovar typhimurium strain VNP20009 with modified lipid A (msbB-) and purine auxotrophic mutation (purI-).SELECTED DRAWING: None

The application provides a rapid, efficient, low-cost and visual detection method for detecting Salmonella based on a LAMP-CRISPR / Cas12a system, and relates to the technical field of pathogenic microorganism detection.The sgRNA specific for detecting Salmonella and the Cas12a assembled complex are used to recognize and combine LAMP amplification products, the collateral cleavage activity of Cas12a is activated, the fluorescently labeled DNA is enzymatically cut, and the fluorescent signal can indicate whether Salmonella is contained in the detection sample.The application provides a high-sensitivity, high-specificity, low-cost, visual and convenient and fast detection method for detecting Salmonella.

The invention provides a salmonella rapid ratio colorimetric detection method based on a hybridization chain reaction, and relates to the technical field of biosensing and nucleic acid detection, and the salmonella is adsorbed and enriched by Fe3O4 (at) PDA (at) PEI magnetic nanoparticles through electrostatic interaction. A salmonella specific hairpin aptamer is used for identifying and triggering a hybridization chain reaction (HCR), and a DNA sequence connected with urease is introduced as a signal detection unit. Phenol red is used as an indicator, and the absorbance and the solution color change in a ratio mode. The kit is rapid, sensitive, specific and easy to use, the sample treatment process is reduced, and the sample detection efficiency is improved; the method can be directly operated at room temperature without complex operation environment, and is convenient to use.

The invention relates to the technical field of salmonella detection, in particular to a multivalent aptamer probe for capturing salmonella, a preparation method of the multivalent aptamer probe and application of the multivalent aptamer probe to salmonella detection. The invention relates to a multivalent aptamer probe for capturing salmonella. The multivalent aptamer probe is prepared from a DNA sample H1, a DNA sample H2, an activation chain, an aptamer and cDNA dry powder through a hybridization chain reaction technology. The multivalent aptamer probe is used in the salmonella detection process, and has the advantages of one-tube detection, no secondary pollution and high detection sensitivity.

The present application relates to the field of biotechnology, in particular to a fusion plasmid and its application in gene transfer and protein expression. The present application provides a novel IncI1 and IncHI2 / IncHI2A type self-transmissible fusion plasmid containing NDM-13 and OXA-10 from clinical Salmonella, which also carries other multiple drug resistance genes and shows resistance to multiple antibiotics. Salmonella is a pathogenic bacterium of zoonosis and has the ability to infect humans and livestock. Salmonella carrying the plasmid has the potential to cause outbreaks, and active research is needed to prevent the occurrence of outbreaks.

The present invention discloses a targeted antimicrobial peptide for Salmonella and a preparation method thereof. The targeted antimicrobial peptide for Salmonella is prepared by selecting a short peptide sequence without positive charge from a plurality of peptide segments with affinity for Salmonella obtained by screening a phage peptide library. The sequence only has the function of binding to Salmonella but does not have bactericidal activity. Based on the affinity sequence, positive charge and hydrophobic amino acids are added to the sequence in combination with the structure-function relationship study. The targeted antimicrobial peptide for Salmonella of the present invention can specifically kill Salmonella, ensuring that other bacteria are not affected while killing Salmonella, allowing beneficial bacteria to survive and maintaining the balance of microbial structure.

The invention discloses a method for analyzing the influence of salmonella infection on chicken liver transcriptome RNA m6A modification, and relates to the technical field of bioengineering, and the method comprises the following steps: animal model establishment and sample collection, RNA extraction, sequencing and data quality control, bioinformatics analysis and differential m6A peaks identification, and data integration and function enrichment analysis. By integrating MeRIP-seq and a transcriptome sequencing technology, a dynamic regulation and control mechanism of RNA m6A modification in the process of infecting the chicken liver with salmonella is disclosed, an m6A modification and gene expression association analysis framework under an infection model is constructed, the analysis depth of an epigenetic regulation and control mechanism of poultry immune response is remarkably improved, and the application prospect is broad. And by establishing a standardized experimental process and a bioinformatics analysis system, accurate identification of differential modification sites and functional association thereof from a transcriptome level is realized, and a new technical path is provided for poultry disease resistance research.

The invention discloses recombinant attenuated salmonella for expressing metalloproteinase TcMMP-13 of ascaris canis and application of the recombinant attenuated salmonella. The recombinant attenuated salmonella is characterized in that the attenuated salmonella expresses the metalloproteinase TcMMP-13. The vaccine has no obvious influence on mouse body health, the insect reduction rate is far higher than the insect reduction rate threshold values of 40% and 50% provided by the World Health Organization (WHO) in schistosome and hookworm vaccine research and development guidelines, and the recombinant attenuated salmonella vector oral vaccine has excellent protection effect and potential application value.

The present disclosure provides formulations, kits, and vaccines directed to immunization of animals against Salmonella. Methods of usng the formulations, kits, and vaccines for protection of avians against one or more species of Salmonella are also provided.

The invention discloses a mesenchymal stem cell loaded with attenuated salmonella, which is prepared by the following steps: loading attenuated salmonella VNP by using a mesenchymal stem cell (MSC) to obtain an MSC-VNP cell; the attenuated salmonella is attenuated salmonella typhimurium VNP20009 and a derivative or gene modified strain thereof, and the strain is an expression plasmid which does not carry or carries an expression therapeutic gene and a tracer protein exogenous gene; the therapeutic gene is a coding gene of a protein which can be expressed in attenuated salmonella and has a therapeutic effect, and comprises a cell apoptosis anti-tumor gene, an angiogenesis inhibitor gene or an immune checkpoint blocker antibody gene, and the foreign gene of the tracer protein is Dsred; the expression plasmid is provided with a promoter lac which is commonly used in attenuated salmonella; and the plasmid contains an anti-plasmid loss element AT. The mesenchymal stem cell can effectively load the attenuated salmonella, can also effectively release the attenuated salmonella, and can be used as a universal cell therapy carrier, so that the therapy cost is saved.

The application discloses a bacillus mucilaginosus for inhibiting systemic infection type, pneumonia type and neurotype chicken pullorum salmonella and application thereof. The bacillus mucilaginosus for inhibiting systemic infection type, pneumonia type and neurotype chicken pullorum salmonella is named Anti-S.gallinarum, is preserved in the China Center for Type Culture Collection, and has a preservation number of CCTCC NO: M20251300.

The invention discloses a novel application of rutin in preparation of a salmonella type III secretion system (T3SS) inhibitor. By constructing a salmonella mouse infection model, the rutin is proved to be capable of remarkably improving the survival rate of infected mice, reducing the bacterial load of liver and spleen target organs and effectively relieving the tissue damage of intestinal tracts, livers and spleens. In-vitro tests show that rutin does not affect the growth of bacteria, and the mechanism of the rutin playing a protection role is that the expression of a salmonella T3SS virulence factor is specifically inhibited, so that the pathogenicity of salmonella is weakened. The invention discloses a potential application of rutin as a salmonella T3SS inhibitor, and provides a theoretical basis for development of related anti-infective drugs.

The invention belongs to the field of salmonella detection, and discloses a fluorescent lateral flow chromatography detection method for detecting salmonella, which realizes sensitive detection of salmonella through combination of CsPbBr3 (at) SiO2 with excellent fluorescence performance and Fe3O4 with magnetic response capability. The problems that in the prior art, real-time on-site antibody detection is long in time consumption, labor-consuming, high in cost, unable to quantify and the like are solved. The fluorescent probe IPNs capable of specifically recognizing salmonella is obtained by coating the outside of perovskite quantum dots with silicon dioxide, and meanwhile, the surfaces of Fe3O4 nanoparticles are modified with salmonella antibodies to prepare the magnetic probe IMNs. The former provides a fluorescence signal, and the latter realizes rapid separation of bacteria, so that the detection is more sensitive, rapid and convenient, and is suitable for various scenes. Human eyes are highly sensitive to green fluorescence, preliminary qualitative detection can be realized without a fluorescence detector, the method is suitable for field detection, and the method is excellent in specificity, anti-interference capability and repeatability and has potential in actual detection.

The invention belongs to the technical field of food safety detection, and relates to a method for simultaneously extracting and detecting nucleic acid of listeria monocytogenes and salmonella. The method comprises the following steps: cracking listeria monocytogenes and salmonella by using a bacterial lysis solution and releasing nucleic acid of listeria monocytogenes and salmonella; the nucleic acid of the listeria monocytogenes and the nucleic acid of the salmonella are extracted at the same time through the synthesized magnetic beads Fe3O4 and Al < 3 + >, and the nucleic acid of the listeria monocytogenes and the nucleic acid of the salmonella are detected at the same time through double RPA / RT-RPA and RPA-LFA test paper. The method has the characteristics of rapidness, high sensitivity and low equipment requirement, and has practical application value in on-site instant detection of food safety.

The invention belongs to the technical field of bacterial infection and drug treatment, and discloses an ability of oridonin in combined synergism of anti-salmonella effect of polymyxin. The invention verifies that oridonin and polymyxin are combined to resist salmonella in a macrophage intracellular environment simulation culture medium that oridonin has a remarkable synergistic capability on the salmonella inhibition capability of polymyxin; a growth curve experiment and a combined sterilization curve experiment verify that oridonin enhances the salmonella infection resistance of polymyxin. A novel combined method and a research and development strategy are provided for clinically treating salmonella infection by polymyxin. Compared with a traditional action mechanism that antibiotics directly kill salmonella, oridonin inhibits salmonella infection by effectively enhancing the sterilization effect of polymyxin, bacterial infection can be effectively prevented and treated while the application concentration of polymyxin can be reduced, and salmonella is not prone to being induced to generate related drug resistance.

The invention discloses a method for detecting salmonella in pasteurized milk, relates to the technical field of food detection, and provides a one-step rapid high-sensitivity detection method suitable for salmonella in pasteurized milk by constructing a platinum-based polymer nano-enzyme composite probe and combining immunomagnetic bead separation and signal amplification strategies. Based on the excellent peroxidase-like activity of the platinum-based polymer nano-enzyme, the composite probe can catalyze a chromogenic substrate to generate a strong signal amplification effect in the presence of low-concentration target bacteria, so that the detection limit of salmonella in a pasteurized milk matrix as low as 10 CFU / mL is realized; compared with a traditional culture method and part of existing immunological methods, the sensitivity is improved by one to two orders of magnitude, and the detection capacity of low-pollution-level salmonella is remarkably enhanced.

This application relates to the field of Salmonella detection technology, and more particularly to a multivalent aptamer probe for capturing Salmonella, its preparation method, and its application in detecting Salmonella. A multivalent aptamer probe for capturing Salmonella is prepared using hybridization chain reaction technology from a DNA sample H1, a DNA sample H2, an activation strand, an aptamer, and cDNA dry powder. This multivalent aptamer probe, when used in the detection of Salmonella, offers the advantages of single-tube detection, no secondary contamination, and high sensitivity.

The invention provides a double probe for detecting salmonella as well as a preparation method and application of the double probe, and belongs to the technical field of bacterial detection. The double probes provided by the invention comprise an aptamer-phage-magnetic bead probe MB (at) Phage (at) Apt and an fDNA-gold nanoparticle probe AuNPs (at) fDNA; the sequence of the aptamer is as shown in SEQ ID NO: 1, and the sequence of the fDNA is as shown in SEQ ID NO: 2. The double probes are assisted by an ultraviolet lamp, on-site rapid visual detection of salmonella is realized through system color change caused by change of fluorescence intensity, the provided detection method is simple to operate, low in cost, less in time consumption and easy to realize, and on-site rapid visual detection of salmonella can be realized without large experimental equipment; a data analysis system and a linear equation are further utilized for calculation, the content of salmonella can be detected in a short time, and the detection efficiency and accuracy are improved.

The invention provides a salmonella identification culture medium and a preparation method thereof. The salmonella identification culture medium comprises the following components: a basic substrate, a sugar agent, a chromogenic substrate and a selective inhibitor. The chromogenic substrate comprises sodium thiosulfate and ammonium ferric citrate. The selective inhibitor is prepared from sodium sulfite, lauryl sodium sulfate, 7-ethyl-2-methyl-4-undecyl sodium sulfate and vancomycin. According to the salmonella identification culture medium, the identification efficiency of salmonella can be effectively improved, and the identification accuracy of salmonella is improved.