14 results about "Salmonella Be" patented technology

The invention discloses application of a method for enhancing the treatment effect of salmonella bacteriophage, and relates to the technical field of microorganisms. According to the method, bacteriophage is added into salmonella for in-vitro culture, after continuous subculture, the salmonella is dropwise added to an SS plate containing the bacteriophage, and resistant bacteria of the bacteriophage are screened. The split bacteriophage is screened aiming at the resistant bacteria of the bacteriophage, and the split bacteriophage and the initial bacteriophage form a bacteriophage cocktail formula, so that the salmonellosis can be well prevented and treated, and the generation of the resistant bacteria is delayed. The method has the beneficial effects that compared with the existing method for screening the bacteriophage-resistant bacteria through a double-layer plate method, the method is more convenient, the operation is simple, and the screening efficiency of the bacteriophage-resistant bacteria is greatly improved. The bacteriophage cocktail contains the bacteriophage for cracking the resistant bacteria, so that the treatment effect of the bacteriophage is improved, and the practical application scene is quite high.

The present disclosure provides formulations, kits, and vaccines directed to immunization of animals against Salmonella. Methods of usng the formulations, kits, and vaccines for protection of avians against one or more species of Salmonella are also provided. For instance, the formulations, kits, and vaccines can induce an immune response in an animal against a Salmonella infection in which the Salmonella infection is caused by more than one Salmonella group bacterium (e.g., a Group B bacterium, a Group C bacterium, and a Group D bacterium).

The application belongs to the field of feed additives, and particularly discloses application of sodium butyrate in preparation of a feed additive against chicken white dysentery Salmonella, and sodium butyrate is added in chicken basic feed as the feed additive against chicken white dysentery Salmonella; the addition of sodium butyrate in chicken basic feed can significantly reduce the bacterial load of chicken white dysentery Salmonella infection in chicken liver and spleen, significantly increase the ratio of villus length to crypt depth of the cecum of chicken infected with chicken white dysentery Salmonella, and improve the intestinal absorption capacity; the content of CD3+ T cells, TCR+ T cells, macrophages and B cells in the liver of chicken infected with chicken white dysentery Salmonella can be significantly reduced; and then the body weight of chicken infected with chicken white dysentery Salmonella is significantly increased, and the survival rate of chicken infected with chicken white dysentery Salmonella is increased. In summary, the addition of sodium butyrate in chicken basic feed can enhance the resistance to chicken white dysentery Salmonella infection.

The invention relates to the technical field of biological detection, and discloses a salmonella monoclonal antibody, a salmonella colloidal gold immunochromatography test strip based on the antibody and a preparation method of the salmonella colloidal gold immunochromatography test strip. Salmonella is used as an antigen, a polyclonal antibody and a monoclonal antibody are prepared respectively, the monoclonal antibody 8E71C8 is screened out, and the salmonella colloidal gold immunochromatography test strip is prepared. The purified monoclonal antibody is used as a 30 nm colloidal gold marker, the optimal pH and the optimal antibody labeling amount of binding of the monoclonal antibody and colloidal gold and the concentration of the polyclonal antibody used as a detection line are optimized, the prepared salmonella colloidal gold immunochromatography test strip is high in specificity, the sensitivity is about 1 * 10 < 4 > CFU / mL and is higher than the average detection lower limit of domestic similar products, and the salmonella colloidal gold immunochromatography test strip can be used for detecting salmonella. And the test strip is simple to operate and high in efficiency, can be used as a means for rapidly and visually detecting the salmonella, and has popularization and application values.

To provide a novel treatment method for benign nervous system tumors, including schwannomas; and to provide a composition for use in the treatment method.SOLUTION: Provided herein are methods for a treating a subject having or at risk of having a benign nervous system tumor. The methods include administering to the subject a therapeutically effective amount of a composition comprising live attenuated Salmonella bacteria, optionally in combination with an immune checkpoint inhibitor and / or angiogenesis inhibitor. Preferably, the attenuated Salmonella is an attenuated strain of S. typhimurium, e.g., Salmonella enterica serovar typhimurium strain VNP20009 with modified lipid A (msbB-) and purine auxotrophic mutation (purI-).SELECTED DRAWING: None

The application provides a rapid, efficient, low-cost and visual detection method for detecting Salmonella based on a LAMP-CRISPR / Cas12a system, and relates to the technical field of pathogenic microorganism detection.The sgRNA specific for detecting Salmonella and the Cas12a assembled complex are used to recognize and combine LAMP amplification products, the collateral cleavage activity of Cas12a is activated, the fluorescently labeled DNA is enzymatically cut, and the fluorescent signal can indicate whether Salmonella is contained in the detection sample.The application provides a high-sensitivity, high-specificity, low-cost, visual and convenient and fast detection method for detecting Salmonella.

The invention provides a salmonella rapid ratio colorimetric detection method based on a hybridization chain reaction, and relates to the technical field of biosensing and nucleic acid detection, and the salmonella is adsorbed and enriched by Fe3O4 (at) PDA (at) PEI magnetic nanoparticles through electrostatic interaction. A salmonella specific hairpin aptamer is used for identifying and triggering a hybridization chain reaction (HCR), and a DNA sequence connected with urease is introduced as a signal detection unit. Phenol red is used as an indicator, and the absorbance and the solution color change in a ratio mode. The kit is rapid, sensitive, specific and easy to use, the sample treatment process is reduced, and the sample detection efficiency is improved; the method can be directly operated at room temperature without complex operation environment, and is convenient to use.

The present application relates to the field of biotechnology, in particular to a fusion plasmid and its application in gene transfer and protein expression. The present application provides a novel IncI1 and IncHI2 / IncHI2A type self-transmissible fusion plasmid containing NDM-13 and OXA-10 from clinical Salmonella, which also carries other multiple drug resistance genes and shows resistance to multiple antibiotics. Salmonella is a pathogenic bacterium of zoonosis and has the ability to infect humans and livestock. Salmonella carrying the plasmid has the potential to cause outbreaks, and active research is needed to prevent the occurrence of outbreaks.

The invention discloses a method for analyzing the influence of salmonella infection on chicken liver transcriptome RNA m6A modification, and relates to the technical field of bioengineering, and the method comprises the following steps: animal model establishment and sample collection, RNA extraction, sequencing and data quality control, bioinformatics analysis and differential m6A peaks identification, and data integration and function enrichment analysis. By integrating MeRIP-seq and a transcriptome sequencing technology, a dynamic regulation and control mechanism of RNA m6A modification in the process of infecting the chicken liver with salmonella is disclosed, an m6A modification and gene expression association analysis framework under an infection model is constructed, the analysis depth of an epigenetic regulation and control mechanism of poultry immune response is remarkably improved, and the application prospect is broad. And by establishing a standardized experimental process and a bioinformatics analysis system, accurate identification of differential modification sites and functional association thereof from a transcriptome level is realized, and a new technical path is provided for poultry disease resistance research.

The invention discloses recombinant attenuated salmonella for expressing metalloproteinase TcMMP-13 of ascaris canis and application of the recombinant attenuated salmonella. The recombinant attenuated salmonella is characterized in that the attenuated salmonella expresses the metalloproteinase TcMMP-13. The vaccine has no obvious influence on mouse body health, the insect reduction rate is far higher than the insect reduction rate threshold values of 40% and 50% provided by the World Health Organization (WHO) in schistosome and hookworm vaccine research and development guidelines, and the recombinant attenuated salmonella vector oral vaccine has excellent protection effect and potential application value.

The application discloses a bacillus mucilaginosus for inhibiting systemic infection type, pneumonia type and neurotype chicken pullorum salmonella and application thereof. The bacillus mucilaginosus for inhibiting systemic infection type, pneumonia type and neurotype chicken pullorum salmonella is named Anti-S.gallinarum, is preserved in the China Center for Type Culture Collection, and has a preservation number of CCTCC NO: M20251300.

The invention belongs to the technical field of food safety detection, and relates to a method for simultaneously extracting and detecting nucleic acid of listeria monocytogenes and salmonella. The method comprises the following steps: cracking listeria monocytogenes and salmonella by using a bacterial lysis solution and releasing nucleic acid of listeria monocytogenes and salmonella; the nucleic acid of the listeria monocytogenes and the nucleic acid of the salmonella are extracted at the same time through the synthesized magnetic beads Fe3O4 and Al < 3 + >, and the nucleic acid of the listeria monocytogenes and the nucleic acid of the salmonella are detected at the same time through double RPA / RT-RPA and RPA-LFA test paper. The method has the characteristics of rapidness, high sensitivity and low equipment requirement, and has practical application value in on-site instant detection of food safety.

The invention discloses a method for detecting salmonella in pasteurized milk, relates to the technical field of food detection, and provides a one-step rapid high-sensitivity detection method suitable for salmonella in pasteurized milk by constructing a platinum-based polymer nano-enzyme composite probe and combining immunomagnetic bead separation and signal amplification strategies. Based on the excellent peroxidase-like activity of the platinum-based polymer nano-enzyme, the composite probe can catalyze a chromogenic substrate to generate a strong signal amplification effect in the presence of low-concentration target bacteria, so that the detection limit of salmonella in a pasteurized milk matrix as low as 10 CFU / mL is realized; compared with a traditional culture method and part of existing immunological methods, the sensitivity is improved by one to two orders of magnitude, and the detection capacity of low-pollution-level salmonella is remarkably enhanced.

The invention provides a double probe for detecting salmonella as well as a preparation method and application of the double probe, and belongs to the technical field of bacterial detection. The double probes provided by the invention comprise an aptamer-phage-magnetic bead probe MB (at) Phage (at) Apt and an fDNA-gold nanoparticle probe AuNPs (at) fDNA; the sequence of the aptamer is as shown in SEQ ID NO: 1, and the sequence of the fDNA is as shown in SEQ ID NO: 2. The double probes are assisted by an ultraviolet lamp, on-site rapid visual detection of salmonella is realized through system color change caused by change of fluorescence intensity, the provided detection method is simple to operate, low in cost, less in time consumption and easy to realize, and on-site rapid visual detection of salmonella can be realized without large experimental equipment; a data analysis system and a linear equation are further utilized for calculation, the content of salmonella can be detected in a short time, and the detection efficiency and accuracy are improved.