Inert carrier salmonella and potential application thereof

A Salmonella, inert carrier technology, applied in the field of biomedical technology detection, can solve problems such as the promotion of disease purification work that affects the effect of disease purification, influence, interfere with detection and diagnosis results, etc., achieve huge application value and market prospects, improve specificity Sexual bottleneck, improve the effect of specific bottleneck

Active Publication Date: 2019-09-10
安徽荣达生物技术有限公司
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  • Abstract
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  • Claims
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Problems solved by technology

[0005] The fundamental reason why the accuracy of agglutination antigen detection results needs to be improved is that the agglutination antigens currently used in agglutination tests are all bacterial antigens, and their components are compounded with bacterial particle antigens of multiple components, rather than a single antigen. Theoretically, this multi-component The antigens in the test are likely to have non-specific cross-reactions with bacteria of the same family, other species, and other components. In view of the working concentration of agglutinated antigens and the number of bacteria, this drawback will inevitably affect or even significantly interfere with the detection and diagnosis results, seriously affecting Disease purification effect and promotion of disease purification work

Method used

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  • Inert carrier salmonella and potential application thereof
  • Inert carrier salmonella and potential application thereof
  • Inert carrier salmonella and potential application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0018] Embodiment 1: Isolation and identification of Salmonella S9

[0019] The 360-day-old healthy layer hens were collected from the 2nd and 4th buildings of the first poultry farm of Mashan Poultry Farm in Wuxi, Jiangsu Province on October 18, 2016. The chickens were placed in an ultra-clean table and cleaned with 75% alcohol Disinfect the surface of the chicken body that has been dehaired in advance, aseptically collect the liver, spleen, intestinal tract and other tissues of the chicken, place them in a sterile glass petri dish and grind them thoroughly, suck the homogenate into a sterile test tube, and add it to BPW at 37°C Incubate overnight with shaking. Aspirate 1 mL of the culture solution and inoculate it in selenite cystine enrichment solution (SC) for selective culture. Use an inoculation loop to draw up the enrichment solution, streak and inoculate it on a xylose lysine deoxycholate (XLD) plate, place it in a 37°C incubator for overnight culture, and select susp...

Embodiment 3

[0026] Example 3: Verification that there is no non-specific agglutination between the inert carrier Salmonella S9 and chicken-derived serum

[0027]Transfer the inert carrier bacteria S9 to LB liquid medium, shake overnight, and centrifuge at 4000rpm for 10 minutes in a 4°C centrifuge the next day, discard the supernatant, resuspend and wash with sterile PBS for three times, and then resuspend to different working Concentration Bacteria Quantity Gradient. Before the test, the bacterial solution was mixed with a vortex instrument, and the agglutination test was performed with sterile PBS / normal saline and SPF chicken serum to ensure that the bacterial solution had no self-agglutination and no non-specific agglutination. In the ultra-clean bench (20-25°C), take several pieces of ordinary glass plates with a clean surface, resuspend and wash the carrier bacteria by centrifugation and washing for 3 times with PBS pre-cooled to 4°C, and then resuspend and dilute to the specified b...

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Abstract

The invention relates to an inert carrier salmonella and potential application thereof. The inert carrier salmonella is expected to be developed into a novel inert carrier bacterium. The inert carriersalmonella can be applied to development of an indirect agglutination test detection method for conveniently and rapidly detecting an antigen or an infection antibody. The inert carrier salmonella ispreserved at the China General Microbiological Culture Collection Center (CGMCC) at present, the preservation address is Beijing, China, the preservation number is CGMCC No.17340, the preservation date is March 18th, 2019, the classification name is Salmonella sp., and the strain number is S9. The salmonella is separated from a healthy chicken flock, the inert carrier is characterized in that bacteria under the high working concentration and serum of various chicken sources under different genetic backgrounds are not subjected to any macroscopic agglutination reaction, in other words, the bacteria and the chicken source serum are not subjected to a nonspecific agglutination reaction, and the salmonella is named as the inert carrier salmonella. The inert carrier salmonella and the potential application thereof are used for developing the novel convenient and rapid indirect agglutination detection method.

Description

[0001] field of invention [0002] The invention belongs to the field of biomedical technology detection, relates to a Salmonella with inert carrier characteristics and its isolation and identification process and potential application, and reveals that the inert carrier Salmonella does not agglutinate non-specifically with chicken serum under the working concentration of bacteria The characteristics of the reaction, the isolate can be used as an inert carrier bacteria and has potential application prospects in the development of indirect agglutination test detection methods for simple and rapid detection of antigens or infection antibodies. Background technique [0003] The agglutination test is a classic rapid immunological diagnosis method widely used in medicine and veterinary medicine. Its principle is that after the granular antigens such as bacteria and red blood cells bind to the corresponding antibodies, when there is electrolyte, the antigen particles will agglutinate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20G01N33/569C12R1/42
CPCG01N33/56916C12R2001/42C12N1/205G01N33/554G01N2333/255
Inventor 朱国强杨斌羊扬孟霞夏芃芃段强德朱晓芳
Owner 安徽荣达生物技术有限公司
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