An inert carrier Salmonella and its potential application

A Salmonella and inert carrier technology, applied in the field of biomedical technology detection, can solve the problems of affecting and affecting the effect of epidemic disease purification, progress of epidemic disease purification work, interference detection and diagnosis results, etc., to achieve improved specificity bottlenecks, huge application value and Market Prospects, Effects of Improving Specificity Bottlenecks

Active Publication Date: 2020-04-03
安徽荣达生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The fundamental reason why the accuracy of agglutination antigen detection results needs to be improved is that the agglutination antigens currently used in agglutination tests are all bacterial antigens, and their components are compounded with bacterial particle antigens of multiple components, rather than a single antigen. Theoretically, this multi-component The antigens in the test are likely to have non-specific cross-reactions with bacteria of the same family, other species, and other components. In view of the working concentration of agglutinated antigens and the number of bacteria, this drawback will inevitably affect or even significantly interfere with the detection and diagnosis results, seriously affecting Disease purification effect and promotion of disease purification work

Method used

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  • An inert carrier Salmonella and its potential application
  • An inert carrier Salmonella and its potential application
  • An inert carrier Salmonella and its potential application

Examples

Experimental program
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Effect test

Embodiment 1

[0018] Embodiment 1: Isolation and identification of Salmonella S9

[0019] The 360-day-old healthy layer hens were collected from the 2nd and 4th buildings of the first poultry farm of Mashan Poultry Farm in Wuxi, Jiangsu Province on October 18, 2016. The chickens were placed in an ultra-clean table and cleaned with 75% alcohol Disinfect the surface of the chicken body that has been dehaired in advance, aseptically collect the liver, spleen, intestinal tract and other tissues of the chicken, place them in a sterile glass petri dish and grind them thoroughly, suck the homogenate into a sterile test tube, and add it to BPW at 37°C Incubate overnight with shaking. Aspirate 1 mL of the culture solution and inoculate it in selenite cystine enrichment solution (SC) for selective culture. Use an inoculation loop to draw up the enrichment solution, streak and inoculate it on a xylose lysine deoxycholate (XLD) plate, place it in a 37°C incubator for overnight culture, and select susp...

Embodiment 3

[0026] Example 3: Verification that there is no non-specific agglutination between the inert carrier Salmonella S9 and chicken-derived serum

[0027]Transfer the inert carrier bacteria S9 to LB liquid medium, shake overnight, and centrifuge at 4000rpm for 10 minutes in a 4°C centrifuge the next day, discard the supernatant, resuspend and wash with sterile PBS for three times, and then resuspend to different working Concentration Bacteria Quantity Gradient. Before the test, the bacterial solution was mixed with a vortex instrument, and the agglutination test was performed with sterile PBS / normal saline and SPF chicken serum to ensure that the bacterial solution had no self-agglutination and no non-specific agglutination. In the ultra-clean bench (20-25°C), take several pieces of ordinary glass plates with a clean surface, resuspend and wash the carrier bacteria by centrifugation and washing for 3 times with PBS pre-cooled to 4°C, and then resuspend and dilute to the specified b...

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Abstract

The invention relates to an inert carrier Salmonella and its potential application, which is expected to be developed into a new type of inert carrier bacteria, which can be applied to the development of an indirect agglutination test method for simple and rapid detection of antigen or infection antibody. The inert carrier Salmonella has been preserved in the China General Microorganism Culture Collection Management Center (CGMCC), the preservation address is Beijing, China, the preservation number is CGMCCNo.17340, the preservation date is March 18, 2019, and the classification is named Salmonella ( Salmonella sp. ), strain code S9. The Salmonella is isolated from healthy chickens, and its inert carrier characteristic is that the number of bacteria at a higher working concentration does not have any visible agglutination reaction with various chicken-derived sera of different genetic backgrounds, that is, it does not produce non-specific agglutination reactions with chicken-derived serum Heterotropic agglutination, we call the Salmonella inert carrier Salmonella. The invention provides an inert carrier bacterium and its potential application for the development of a novel simple and rapid indirect agglutination detection method.

Description

[0001] field of invention [0002] The invention belongs to the field of biomedical technology detection, relates to a Salmonella with inert carrier characteristics and its isolation and identification process and potential application, and reveals that the inert carrier Salmonella does not agglutinate non-specifically with chicken serum under the working concentration of bacteria The characteristics of the reaction, the isolate can be used as an inert carrier bacteria and has potential application prospects in the development of indirect agglutination test detection methods for simple and rapid detection of antigens or infection antibodies. Background technique [0003] The agglutination test is a classic rapid immunological diagnosis method widely used in medicine and veterinary medicine. Its principle is that after the granular antigens such as bacteria and red blood cells bind to the corresponding antibodies, when there is electrolyte, the antigen particles will agglutinate...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20G01N33/569C12R1/42
CPCG01N33/56916C12R2001/42C12N1/205G01N33/554G01N2333/255
Inventor 朱国强杨斌羊扬孟霞夏芃芃段强德朱晓芳
Owner 安徽荣达生物技术有限公司
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