Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Lactobacillus rhamnosus derived from human breast milk and application of lactobacillus rhamnosus

A technology of Lactobacillus rhamnosus and bacterial agent, applied in the direction of Lactobacillus, application, bacteria used in food preparation, etc., can solve the problems of unsatisfactory application effect of probiotic lactobacillus, and achieve the goal of maintaining intestinal health and inhibiting growth. Effect

Inactive Publication Date: 2017-05-31
FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
View PDF3 Cites 16 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The application effect of many probiotics such as lactic acid bacteria is not ideal

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lactobacillus rhamnosus derived from human breast milk and application of lactobacillus rhamnosus
  • Lactobacillus rhamnosus derived from human breast milk and application of lactobacillus rhamnosus
  • Lactobacillus rhamnosus derived from human breast milk and application of lactobacillus rhamnosus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1. Isolation and identification of Lactobacillus rhamnosus Z5 derived from human breast milk

[0047] 1. Isolation of Lactobacillus rhamnosus Z5 derived from human breast milk

[0048] 1. Collection of human breast milk samples

[0049] Mature breast milk samples (10 days after delivery) were provided by full-term and normal delivery women in Beijing, who were in good health and had no diseases such as mastitis. The breast milk sampling method refers to the method invented by Martín and is slightly improved. Before sampling, the nipple and areola are washed with sterile water, and the first 500 μL of breast milk is discarded, and 5-10 ml of mature breast milk is collected in a sterile centrifuge tube. At the same time, a sterilized cotton swab was used to take a skin sample from the nipple and areola, and the sample was placed in an anaerobic ice box and immediately sent to the laboratory for isolation and screening (the time from sampling to strain isolation sho...

Embodiment 2

[0093] Example 2, Lactobacillus rhamnosus Z5 as the in vitro activity evaluation of probiotics

[0094] 1. Determination of acid tolerance of Z5 strain

[0095] Lactobacillus rhamnosus Z5 was inoculated in MRS medium with different pH values ​​(pH 2.5, pH 3.0, pH 6.5), and cultured under anaerobic conditions at 37°C for 0h, 3h, and 6h, with three parallels for each group. Bacteria counting method was used to observe the acid resistance of the strain and calculate the survival rate.

[0096] The results are shown in Table 4, the number of live bacteria lg cfu / ml of the Z5 strain changed from 7.25 at the initial 0h to 6.35 after 6h after 6h under the pH 2.5 condition, and the survival rate was 87.58%. After 6h under the pH 3.0 condition, the viable bacteria The number lg cfu / ml value changed from 7.91 at the initial 0h to 7.75 after 6h, and the survival rate of the strain still reached 97.98%, which indicated that the Z5 strain had strong acid tolerance.

[0097] Table 4 Survi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses lactobacillus rhamnosus derived from human breast milk and application of the lactobacillus rhamnosus. The lactobacillus rhamnosus Z5 disclosed by the invention is initially derived from the human breast milk and belongs a new subspecies of the lactobacillus rhamnosus. An experiment proves that the strain has the properties of acid resistance, bile salt resistance, and resistance on a simulated animal gastrointestinal tract environment, capability of inhibiting growth harmful bacteria including escherichia coli and salmonella in intestines and capability of being stuck on epithelial cells of human and animal intestines very well. The lactobacillus rhamnosus can be used for preparing health-care foods, fermented foods, vaccine adjuvants and animal feed so that the balance of intestinal flora of human and animals is easy to adjust and the health of the intestines of the human and animals is increased.

Description

technical field [0001] The invention belongs to the technical fields of food industry technology and microbial strain screening, and in particular relates to a strain of Lactobacillus rhamnosus derived from human breast milk and its application. Background technique [0002] Probiotics are live microorganisms that, when taken in appropriate doses, have beneficial health effects on the host human or animal. Due to the immature development of the immune system of infants and young children, maternally-inherited antibodies gradually disappear after birth, and are in a susceptible period from birth to 6 years old. During this period, most infants will suffer from gastrointestinal and immune dysfunction , lead to weak body resistance, digestive disorders, and intestinal malabsorption and infectious diseases. These abnormal physiological states not only hinder the growth and development of the baby's body, but also have different degrees of impact on the baby's intellectual devel...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L33/135A23K10/18A61K39/39C12R1/225
CPCA61K39/39A61K2039/55594A23V2002/00C12R2001/225C12N1/205A23V2400/175A23V2200/3204
Inventor 乔宇高盛石波彭晴徐小轻张宇微
Owner FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com