Salmonella sensor, preparation method and detection method of salmonella concentration

A Salmonella and sensor technology, applied in the field of biosensing, can solve the problems of expensive equipment, low sensitivity, and cumbersome sample processing process, and achieve the effect of simple equipment, easy miniaturization, and high-sensitivity detection of Salmonella concentration

Active Publication Date: 2017-05-31
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows salmons to quickly measure their presence accurately with very small devices that are cost-effective at making them smaller than traditional methods like enzyme immunoassay or PCR techniques.

Problems solved by technology

This patents describes different ways to quickly identify salmolithiasis due to its potential harmful effects from ingestible organisms likeSalomonas spp., Escherichia coli, Yersiniae sporans, Bacillaria cerevisae, Listeriosea chloroformis, Staphylococcus pseudonitidum, Helminthosporanicola, Campylobacina, Toxic Bacteraemia Syndrome Viruses, Enteroviridae, Norcardiella tarda, Cryptophaga cariniphiliaceae, Chlamydimethylamine, Cyclosarin peptides, Dysbacco mosaacearboxymetry Group 2 protein family members involved with saltwater fish diseases including Saltwater Sheep Fever disease, Sweet Water Hamster Ovale syndrome, Fish Plague Black Spotted fever virus, Aquatic Animal Hepatitis A Vimsulagel et al.; Pathogens associated with these illnesses: Salmonellosis, Shigella sonnecrosis, Hemolytic Strecker Anemothrix Narrow Stainting Homologue System, Microbial Food Safety Authority National Laboratory's Standards Publication No. 97-5001 discusses how current diagnoses systems have limitations in terms of speed and sensitivity, making them difficult to use effectively during emergency situations where there may be significant risk of exposure.

Method used

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  • Salmonella sensor, preparation method and detection method of salmonella concentration
  • Salmonella sensor, preparation method and detection method of salmonella concentration
  • Salmonella sensor, preparation method and detection method of salmonella concentration

Examples

Experimental program
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Embodiment 1

[0053] Example 1 Salmonella sensor based on steric hindrance effect

[0054] Principle: The gate electrode is an ITO electrode assembled with cadmium sulfide quantum dots (CdS QDs). Under light conditions, when the energy of the light is greater than the energy required for the electron transition in CdS, the electrons in the valence band in CdS will transition to the conduction band. , forming electron-hole pairs. When the electrons in the conduction band are injected into the electrode, the electron donor in the solution provides electrons to the holes in the valence band, which will form a photocurrent. The generation of this current will reduce the potential of the electrolyte / gate electrode interface, thereby increasing the applied on the OECT device. effective gate voltage. The channel current of OECT is given by the following equation:

[0055]

[0056] where q stands for electron charge, µ stands for hole mobility, represents the initial hole density in the orga...

Embodiment 2

[0066] Example 2 Salmonella immunosensor based on exciton-plasma effect

[0067] Principle: Based on the steric hindrance effect, a system based on the exciton-plasmon effect between cadmium sulfide quantum dots (CdS QDs) and gold nanoparticles (AuNPs) is designed to further improve the sensitivity of the sensor, due to the CdS QDs The fluorescence spectrum overlaps with the ultraviolet absorption spectrum of Au NPs, and the fluorescence of CdS QDs under light conditions can excite Au NPs to undergo surface plasmon resonance, and the interaction between them will change the excitonic state in CdS QDs, resulting in a decrease in photocurrent. Therefore, Au NPs were modified on Salmonella, and antibodies were connected to the gate electrode. After specific binding, the exciton-plasmon effect was excited, resulting in a decrease in photocurrent. ds The -T curve shows the reduction of the light "off-on" step, and the effect of detecting different concentrations of Salmonella can b...

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Abstract

The invention discloses a salmonella sensor, a preparation method and a detection method of salmonella concentration. The salmonella sensor comprises an electrolytic tank, an electrolyte which is arranged in the electrolytic tank, an organic electrochemical transistor which is arranged in the electrolytic tank and a gate electrode which is arranged in the electrolytic tank, wherein the organic electrochemical transistor comprises a substrate, a source electrode and a drain electrode which are arranged on the substrate and an organic semiconductor film layer which coats the substrate and connects the source electrode and the drain electrode; a photoelectric active semiconductor material modifies the gate electrode as a sensitive function layer of the sensor; and an antibody combined with salmonella is fixed on the gate electrode modified by the photoelectric active semiconductor material. The salmonella sensor disclosed by the invention has extremely high sensitivity and extremely low detection limit, and is simple and easy to microminiaturize. The detection method of salmonella concentration based on the salmonella sensor can realize fast and simple high-sensitivity salmonella concentration detection.

Description

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Claims

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Application Information

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Owner SHENZHEN UNIV
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