Antibacterial peptide of heterometrus spinifer and application of antibacterial peptide

A technology of antimicrobial peptides and uses, applied in the direction of antibacterial drugs, peptide sources, applications, etc., can solve the problems of increasing drug-resistant bacteria, treatment failure of human and animal infectious diseases, etc., and achieve good antibacterial effect

Inactive Publication Date: 2013-08-28
CHINA UNIV OF GEOSCIENCES (WUHAN)
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Problems solved by technology

Because long-term low-dose antibiotic use in food animals will increase drug-resistant bacteria, and bacterial drug-resistant genes can be passed between bacteria in humans, bac

Method used

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  • Antibacterial peptide of heterometrus spinifer and application of antibacterial peptide
  • Antibacterial peptide of heterometrus spinifer and application of antibacterial peptide
  • Antibacterial peptide of heterometrus spinifer and application of antibacterial peptide

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Embodiment Construction

[0018] The present invention will be described in detail below in conjunction with specific embodiments, but the protection content of the present invention is not limited to the following embodiments.

[0019] Preparation of Peptocin antimicrobial peptide gene:

[0020] A: Extraction of total RNA from scorpion venom glands (RNAiso Plus, purchased from Takara)

[0021]1. Take the scorpion glands that have been electrocuted and quickly transfer them to a mortar pre-cooled with liquid nitrogen, grind the tissue with a pestle, and add liquid nitrogen continuously until it is ground into powder (if it is not ground thoroughly, it will affect the absorption of RNA. rate and quality). Add an appropriate amount of RNAiso Plus that matches the homogenate volume of the sample into the mortar and homogenize thoroughly. 2. Transfer the homogenate to a centrifuge tube and let stand at room temperature (15-30°C) for 5 minutes. 3. Add chloroform (1 / 5 volume of RNAiso Plus) to the homogen...

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Abstract

The invention provides a Peptocin antibacterial peptide separated from heterometrus spinifer and obtained through a chemical synthesis method. The antibacterial peptide has a remarkable antibacterial effect on clinically-separated multi-drug resistant acinetobacter baumannii, and can be used for efficiently inhibiting the growth of the drug resistant acinetobacter baumannii; and meanwhile, human red blood cell toxicity tests also show that the antibacterial peptide is small in toxicity and can be applied to the preparation of drugs for treating or preventing diseases caused by the drug resistant acinetobacter baumannii and particularly to the preparation of drugs for treating serious infectious diseases such as ventilator-associated pneumonia, septicemia, meningitis, otitis media, skin soft-tissue infection, urinary system infection, central nervous system infection and the like caused by the drug resistant acinetobacter baumannii. The antibacterial peptide provided by the invention is remarkable in inhibiting effect on gram bacteria, unique in antibacterial mechanism and low in toxicity, and can also be applied to the preparation of drugs for treating or preventing diseases caused by micrococcus luteus, salmonella and other gram bacteria.

Description

technical field [0001] The invention relates to an antimicrobial peptide from Asian rainforest scorpion venom, and also relates to the gene of the antimicrobial peptide. At the same time, the invention also discloses the use of the antimicrobial peptide, which belongs to the field of bioengineering. Background technique [0002] Acinetobacter baumannii is a gram-negative bacillus that does not ferment sugar. It is widely distributed in the environment and the skin surface of healthy people and colonizes the skin, pharynx, respiratory tract and digestive tract of hospitalized patients for a long time. It can survive tenaciously on the surface of medical equipment. It tolerates temperature and pH very well and survives for a long time. Acinetobacter baumannii is an opportunistic pathogen that can easily cause nosocomial infections. According to the domestic and foreign nosocomial infection monitoring data from 2006 to 2007, the detection rate of Acinetobacter baumannii ranks a...

Claims

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Application Information

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IPC IPC(8): C07K14/435C12N15/12A61K38/17A61P31/04
CPCY02A50/30
Inventor 曾宪春聂尧吴诗芬
Owner CHINA UNIV OF GEOSCIENCES (WUHAN)
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