Fast check reagent of Salmonella causing food poisoning

Abstract

The invention relates to a fast check reagent of Salmonella causing food poisoning, in particular to a fast check reagent of food source type Salmonella aertrycke, Salmonella suipestifer, Salmonella enteritidis, TAB vaccine and salmonella B and a preparation method thereof. The fast check reagent has the characteristics of high speed, high accuracy, high sensitivity, and the like. Compared with a traditional method, the fast check reagent has an application value and has an important meaning for guiding clinical diagnosis and treatment and avoiding abusing antibacterial medicines. Specific Salmonella polyvalent and monovalent serum are added in enriched liquid, the check diagnosis process is shortened to 2-6 hours, the serum agglutination reaction of specific serum can be utilized at the time of enriching, and the identification accuracy is larger than 97 percent. The fast check reagent has little time consumption, high sensitivity and strong accuracy when checking pathogenic bacteria.

Description

technical field [0001] The invention relates to a rapid detection reagent for Salmonella that causes food poisoning, especially the most common mouse that has strong viability in the external environment, can survive for several months in water, milk and meat, and reproduces rapidly at the optimum temperature. Rapid detection reagents for Salmonella typhi, Salmonella choleraesuis, Salmonella enteritidis, Salmonella typhi A and B. Salmonella food poisoning is mostly caused by animal foods, especially meat (such as dead livestock meat, cooked meat products), and can also be caused by poultry, eggs, and dairy foods. Mainly acute gastroenteritis, the main symptoms are vomiting, diarrhea, abdominal pain, and the stool is yellow-green watery stool. Fever at 38°C-40°C. Severe patients present with chills, convulsions, convulsions and coma. The elderly, children and the infirm can also lead to death if first aid treatment is not given in time. The traditional routine detection and...

AI Technical Summary

Problems solved by technology

The traditional biochemical identification test cycle is long, the operation is cumbersome, and the quality of the reagents cannot be guaranteed, which seriously affects the confirmation of pathogenic bacteria. It takes experience to select suspicious colonies on the medium for selective isolation and culture, and there is a possibility of missed detection. The stability is very high. Although the processed food cannot be detected sometimes, there may still be enterotoxins, and the conventional detection method of enterotoxins requires animal experiments, which is difficult and takes a long time.

The entire testing and confirmation process takes about 4-15 days