The invention discloses a bactericidal pollution-free pesticide, which is prepared from leucine, valine, proline, methionine, glycocoll, threonine, tyrosine, serine, acetylcysteine, lysine hydrochloride, arginine hydrochloride, histidine monohydrochloride, tryptophan, alanine, glutamic acid, isoleucine, phenylalanine, sorbierite, sodium hydrogensulfite and water. The bactericidal pollution-free pesticide is used for preventing and controlling diseases such as gold diseases, black gold diseases, red gold diseases and the like of cereals, fruit trees and vegetables, does not pollute crops and environment, does not have residual toxicity, is long in lasting time of pesticidal potency, has the effects of prevention and control, and can prevent and control the diseases of the cereals, the fruit trees and the vegetables; and when the bactericidal pollution-free pesticide is applied, the application time is reduced, labors are saved, the drought tolerance and waterlogging tolerance of the cereals, the fruit trees and the vegetables are enhanced, so that root systems are developed, branches and leaves are flourish, healthy and strong, fruits are quick in coloring and early in maturity, the crops grow perfectly, excessive growth is inhibited, and the quality and yield can be improved.