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Method for seedling culture of camellia nitidissima twig cultivation group under sunlight

A golden camellia tea and sunlight technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., to achieve the effects of reducing electricity costs, promoting robust growth, and promoting cell division

Active Publication Date: 2017-09-08
广西壮族自治区南宁良凤江国家森林公园
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no literatures and patents about using sunlight to carry out industrialized seedling cultivation of Camellia japonica seedlings

Method used

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  • Method for seedling culture of camellia nitidissima twig cultivation group under sunlight
  • Method for seedling culture of camellia nitidissima twig cultivation group under sunlight
  • Method for seedling culture of camellia nitidissima twig cultivation group under sunlight

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A method for cultivating tissue culture seedlings of golden camellia twigs under sunlight conditions, comprising the following steps:

[0035] (1) Disinfection of explants and primary culture of buds: In sunny weather for more than 3 consecutive days, the semi-lignified or rigid woody shoots of Camellia camellia with good growth were collected from the Camellia japonica resources nursery in Nanning Arboretum, Guangxi. Cut off the leaves, leave a 1 cm long petiole, brush the shoots with 5‰ washing powder water for 2 minutes, cut the shoots into 3 cm stems containing more than one axillary bud, and lignify the semi-lignified stems and rigid wood. The stems were put into different containers, rinsed with tap water three times, placed on an ultra-clean workbench, sterilized with 75% alcohol solution for 30 s, washed with sterile water for three times, and then placed in 1‰ HgCl 2 In the solution, soak the semi-lignified stems for 6 minutes, soak the rigid-lignified stems fo...

Embodiment 2

[0040] A method for cultivating tissue culture seedlings of golden camellia twigs under sunlight conditions, comprising the following steps:

[0041] (1) Disinfection of explants and primary culture of buds: In sunny weather for more than 3 consecutive days, the semi-lignified or rigid woody shoots of Camellia camellia with good growth were collected from the Camellia japonica resources nursery in Nanning Arboretum, Guangxi. Cut off the leaves, leave a 0.5 cm long petiole, brush the shoots with 5‰ washing powder water for 2 minutes, cut the shoots into 2 cm stems containing more than one axillary bud, and divide the semi-lignified stems and hardwood The thawed stems were put into different containers, rinsed three times with tap water, placed on an ultra-clean workbench, sterilized with 75% alcohol solution for 30 s, washed three times with sterile water, and then placed in 1‰ HgCl 2 In the solution, soak the semi-lignified stems for 6 minutes, soak the rigid lignified stems f...

Embodiment 3

[0046] A method for cultivating tissue culture seedlings of golden camellia twigs under sunlight conditions, comprising the following steps:

[0047] (1) Disinfection of explants and primary culture of buds: In sunny weather for more than 3 consecutive days, the semi-lignified or rigid woody shoots of Camellia camellia with good growth were collected from the Camellia japonica resources nursery in Nanning Arboretum, Guangxi. Cut off the leaves and leave a 0.5 cm long petiole, brush the shoots with 5‰ washing powder water for 2 minutes, cut the shoots into 2.5 cm stems containing more than one axillary bud, and divide the semi-lignified stems and hardwood The thawed stems were put into different containers, rinsed with tap water three times, placed on the ultra-clean workbench, sterilized with 75% alcohol solution for 30 s, washed with sterile water for three times, and then placed in 1‰ HgCl 2 In the solution, soak the semi-lignified stems for 6 minutes, soak the rigid-lignifi...

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Abstract

The invention relates to a method for seedling culture of a camellia nitidissima twig cultivation group under sunlight. The method includes the steps including explant sterilization and budding culture, bud succession proliferation culture, bud germination culture and seedling transplantation. The light source adopted comes from the sunlight, and the effect of the sunlight on the growth of seedling culture of the camellia nitidissima cultivation group is given full play by adjusting light intensity. Under sunlight, bud culture of camellia nitidissima is 40 days, growth coefficient is 9-12, rooting percentage of seedling culture of the camellia nitidissima cultivation group is as high as 90-95%, the rooting percentage is more than 20% of that of the method of using an incandescent lamp as a light source, time for sprouting point under sunlight is 20-25 days, time for sprouting pint under the incandescent lamp is 30-35 days, the former is 5-10 days shorter than the latter, survival rate of transplantation is up to 95%, and requirements of industrial production of seedlings of the camellia nitidissima are met.

Description

technical field [0001] The invention relates to the technical field of asexual rapid propagation of plants, in particular to a method for cultivating tissue-cultured seedlings of camellia japonica twigs under sunlight. Background technique [0002] Camellia ( Camellia nittidissima Chi ), belonging to the genus Camellia in the family Theaceae, is the only species with golden petals in the Camellia family, and is a unique traditional flower in my country. It is also one of the eight national first-level key protected plants and enjoys a high reputation both at home and abroad. Camellia chrysalis is hailed as "the queen of the tea clan" and "the giant panda in the plant world" in China, while it is called "the yellow camellia in fantasy" and "the magical oriental magic tea" in foreign countries. Golden camellia is not only of high ornamental value, but also a precious medicinal material, and its medicinal value has also been widely recognized by the society. Golden camellia ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001A01H4/008
Inventor 施福军刘芳黄艳俞建妹刘玉军孙灿岳沈遐陈碧珍李媚黄秋清黎晓生曾令达许涛
Owner 广西壮族自治区南宁良凤江国家森林公园
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