35 results about "Begonia mariti" patented technology

The invention discloses a cultivating and planting method of a begonia named Yinjiao. According to the invention, an artificial pollination method is adopted, begonia dryadis irmsch. is adopted as a female parent, B.'White King' is adopted as a male parent, and sexual hybridization is carried out; an offspring individual plant is subject to self-pollination, and the variety Yinjiao is obtained through selective breeding from an F2 generation colony; the chromosome numbers of the parents and the filial generation are determined; and the variety Yinjiao obtained through the previous cultivationsteps is planted in humic soil under a temperature of 15-25 DEG C and an air relative humidity of 65-75%, where in the humic soil is air-permeating and is rich in organic matters. Also, the drainage of the humic soil is good.

The invention discloses a brewing method of a begonia root wine. The brewing method is characterized by comprising the steps: picking dry and disease-free begonia roots, and performing soaking, pulping, compound enzyme treating, blending, sterilizing, yeast fermenting, filtering, ageing, canning, sterilizing and the like to brew the begonia root wine. According to the brewing method, by adopting a compound enzyme enzymolysis technology, a pectin layer of the begonia roots can be decomposed, more nutritional ingredients can be fully separated out, the utilization rate of a raw material is increased, and thus the finished begonia root wine is thick and strong in taste, mellow in flavor, stable in color, and rich in nutrients, and has health care functions of activating blood and dissolving stasis, stopping bleeding and clearing heat, and the like.

The invention discloses a method for cultivating begonia fangii using water. The method comprises disinfecting mature leaves, laying a layer of absorbent cotton in an aseptic culture dish, and laying a layer of qualitative filter paper on the absorbent cotton; placing the leaves flatwise on the aseptic qualitative filter paper, soaking the absorbent cotton and the filter paper in a rooting nutrient solution to make the leaves root until 4 to 7 adventitious roots grow up at each petiole; supplementing an indefinite bud induction solution to wet the absorbent cotton and filter paper to make the leaves root until 5 to 10 buds grow up at each rooting portion, supplementing a 1 / 2 MS growth nutrient solution prepared by sterile water to cultivate until small leaves grow up from the germinal bus; and disinfecting the leaves in a shading shed, planting the leaves in a matrix, spraying in the shading shed to raise the humidity in air to obtain begonia fangii. According to the begonia fangii cultivated by the method, the survival rate is 99 to 100%, the germination rate is raised by 40%, the survival rate is raised by 30%, the period is shortened almost one time, so the method has a popularization meaning in breed conservation and production of begonia.

The invention belongs to the seedling breeding field, particularly to a malus crabapples root grafting breeding method. The malus crabapples root grafting breeding method comprises the processes of stock selection, scion strip collection, grafting, grafting seedling planting and grafting seedling management. Main foots and side foots of Hubei crabapples seedlings are selected to serve as the stocks, the root grafting seedling growing is performed by a cleft grafting method, the specification management is performed in the late graft period, and the malus crabapples root grafting high-quality seedlings are cultivated. According to the malus crabapples root grafting breeding method, the grafting can be achieved in the indoor space and the operation is convenient, the efficiency is high, and the labor intensity is greatly reduced in comparison with the field grafting; the ordinary Hubei crabapples roots serve as the stocks and accordingly the resources are rich and the material selection is easy; the main foots and the side foots of the Hubei crabapples serve as the stocks, 6 to 7 root grafting stocks can be cut from a one-year Hubei crabapples seedling generally, and accordingly the propagation coefficient is improved.

The invention discloses a tissue culture method of malus hupehensis. The method comprises five steps, namely, the explant disinfecting step, the primary culture step, the subculture step, the rootingculture step and the acclimatization and transplant step. The method has the advantages that the tissue culture rapid propagation technology is utilized; the outstanding characters of an original stock plant remain; the propagated generations are uniform; in addition, the propagation coefficients of the malus hupehensis can be efficiently and quickly increased, and the propagation cycle is reduced; moreover, the transplanting survival rate of the malus hupehensis is high. The method method meets the market demand of southern region on the malus hupehensis and is beneficial protection for the wild resource of the malus hupehensis. In addition, the culture method is easy to operate, requires low labor and time input, and is suitable for market popularization.

The invention provides a method for extracting phloridzin of malus hupehensis (pamp.) rehd. The method comprises the operation steps as follows: leaves or / and twigs of malus hupehensis (pamp.) rehd are taken and added with water for reflux extraction for three times, for the first time, 8-10 times amount of water is added for reflux extraction for longer than 60 plus or minus 5 min at the temperature of 80 plus or minus 2 DEG C, for the second time, 3-5 times amount of water is added for reflux extraction for longer than 60 plus or minus 5 min at the temperature of 80 plus or minus 2 DEG C, for the third time, 3-5 times amount of water is added for reflux extraction for longer than 60 plus or minus 5 min at the temperature of 80 plus or minus 2 DEG C, and extracting solutions of the three times are combined to obtain phloridzin. With the adoption of the method, only water is taken as a solvent, phloridzin of malus hupehensis (pamp.) rehd can still be effectively extracted, and convenience is provided for large-scale industrial production.

The invention discloses a cultivation method for improving a survival rate of North American begonia and relates to the technical field of floriculture. The method comprises the following steps of 1, seedbed preparation; 2, cutting seedling preparation; 3, cuttage; 4, field planting; 5, field management. According to the cultivation method, the sprouting ratio and the survival rate are higher, the growth speed is high, the flowering period is long, and the method has good economic benefit and social benefit, and is suitable for large area cultivation and popularization.

The invention discloses a tissue culture rapid-propagation method of a fire axillary bud of a North American crabapple tableland. The tissue culture rapid-propagation method comprises the following steps: taking an axillary bud on a spring twig of a fire crabapple of the North American crabapple tableland as an explant, disinfecting and inoculating, then culturing on an initial culture medium till the axillary bud is germinated, and then transferring the explant to a proliferation culture medium for proliferation culture; when a split bud grows to 2-3 cm, transferring to a rooting culture medium for rooting induction to form a complete plant; carrying out seedling adaptation, domestication and transplantation survival on a plantlet to obtain an expanding propagation seedling. The tissue culture rapid-propagation method disclosed by the invention can be used for inducing the axillary bud of the fire crabapple of the North American crabapple tableland by adopting a tissue culture technology and establishing an in-vitro rapid propagation technical system of an axillary bud regenerated plant, thereby keeping the excellent variety and providing the seedlings on a large scale; in addition, the tissue culture rapid-propagation method disclosed by the invention achieves the proliferation rate of more than 340%, the plantlet rooting rate of more than 65% and the survival rate of more than 80%, can be used for accelerating the propagation speed and scale of the fire North American crabapple and has very good practicability.

The invention provides a simple method for breeding aphelinus mali. The simple method is characterized by comprising steps of (1), providing annual malus prunifolia seedlings and planting the malus prunifolia seedlings in cups or pots when 7-8 leaves grow on each malus prunifolia seedling; (2) inoculating 3-5 adult eriosoma lanigerum on the planted malus prunifolia seedlings, placing the malus prunifolia seedlings with the eriosoma lanigerum into screen cages with a large quantity of aphelinus mali which is subjected to eclosion in advance when nymphs of the eriosoma lanigerum on branches of the malus prunifolia seedlings develop into fourth-stage eriosoma lanigerum or adult eriosoma lanigerum, enabling the aphelinus mali to perform parasitism on the malus prunifolia seedlings in the screen cages for 48 hours and then taking out the malus prunifolia seedlings; continuing feeding the eriosoma lanigerum on the malus prunifolia seedlings for 1-2 days after the eriosoma lanigerum turns black, trimming the branches of the malus prunifolia seedlings by pruning shears or brushing off black pupae by brushes. The simple method has the advantage that the aphelinus mali can be quickly and efficiently bred.

The invention provides a Tripterygium hypoglaucum seed propagation method. The method includes the steps of low-temperature storage of seeds, vernalization of the seeds, seed sowing, intertillage management, etc. The step of vernalization of the seeds is as follows: storing the Tripterygium hypoglaucum seeds that have been stored at a low temperature in sand at room temperature in a moisturizing and ventilating condition for one month; and after soaking of the Tripterygium hypoglaucum seeds stored in sand in gibberellin, placing the seeds in a condition with temperature ranging from 7+ / -2 DEG C, and maintaining illumination and humidity for one month. Through exploring and researching, a low-temperature treatment method and a hormone regulation method are adopted to overcome a factor of instability of a seed germination rate. Therefore, the seed germination rate is increased. Moreover, a systematic method is employed to lay a foundation for artificial cultivation in future.

The invention discloses a traditional Chinese medicine composition for treating Qi-stagnation and blood stasis type femoral head necrosis. The traditional Chinese medicine composition is characterized by being prepared from the following medicinal materials in parts by weight: 10 parts of rubiaceae borreria stricta, 10 parts of Chinese astilbe rhizome, 12 parts of Canthium simile Merr., 10 parts of radix zanthoxyli, 12 parts of Hubei begonia root, 12 parts of all-grass of impatiens loulanensis, 15 parts of pothos repens, 8 parts of piper laetispicum, 10 parts of all-grass of phlegmariurus austrosinicus, 15 parts of Jasminum officinale L. var. grandiflorum, 10 parts of kadsura coccinea, 15 parts of Yunnan Schisandra chinensis root, 12 parts of wallich swallowwort root, 15 parts of henry woodbetony root, 12 parts of manyflower fissistigma root or stem, 10 parts of Polygala aureocauda Dunn, 8 parts of maianthemum japonicum, 10 parts of soroseris umbrellata, 10 parts of phacellanthus tubiflorus, 4 parts of deer-horn glue, 4 parts of sea cucumber, 8 parts of leech and 6 parts of liquorice. The traditional Chinese medicine composition disclosed by the invention is used for treating the Qi-stagnation and blood stasis type femoral head necrosis and is high in effective rate and reliable in curative effect, and the femoral head necrosis does not reoccur after healing.

The present invention discloses a composition using Malus hupehensis (Pamp.) Rehd and a bamboo extract as effective components, wherein the effective components of the composition comprise Malus hupehensis (Pamp.) Rehd and a bamboo extract, Malus hupehensis (Pamp.) Rehd is at least one selected from a Malus hupehensis (Pamp.) Rehd plant and a Malus hupehensis (Pamp.) Rehd extract, and the bamboo extract is at least one of the following seven components such as bamboo leaf flavone, bamboo leaf triterpene, bamboo shoot sterol, bamboo shoot oligopeptide, bamboo silicon, bamboo polysaccharide, and bamboo dietary fiber. The invention further provides uses of the composition, wherein the composition can be used for preparing functional food for prevention or treatment of any one disease selected from central obesity, premature / perimenopausal syndrome, Alzheimer disease and depression.

The invention discloses a method for culturing potted fruit tree crabapple, comprising the following concrete steps of farmland selecting, seedling selecting, field planting, soil, fertilizer and water managing and pruning. In April of spring, seedlings are temporarily planted in a selected farmland to serve as parent trees planted in pots in the future; the soil, fertilizer and water management and the pest control are strengthened during the temporary planting period; the trees are pruned and then are planted in the pots until the trunks are 3-5cm thick, and mud pots are preferable when the trees are planted in the pots; the trees are watered after being planted into the pots in time and the potted trees are pruned, and the fertilizer and water management work and the pest control work are strengthened in the later period; the potted trees are pruned in summer to promote flower buds to differentiate; and the potted trees can be sold in the market after blooming and fruiting in the coming year. In the method, garden fruit trees are stunted and then are cultivated in small-sized flowerpots, which can beautify yards and decorating rooms. The fruit trees have the characteristics of small occupied space, wide application range and the like and are convenient to transport. The potted fruit tree crabapple not only has the appreciation of potted plants, but also can be used as a mobile orchard to be picked and has edible value.

Cutting propagation method of begonia leaf regards begonia leaves as cutting slips and regards perlites as cutting medium. Cuttage is proceeded by utilizing a cuttage bed or a cuttage container tray at the height of 60 70 centimeters above the floor. Begonia of stem cuttage can take roots and form shoots in 20 25 days and the rooting rate can reach 90 95 %. Begonia of leaf cuttage can take roots in incision in 15 20 days, produce adventitious buds and form new plants in 60 75 days. And the rooting rate of the Begonia of leaf cuttage can reach 75 85 %. Cutting propagation method of begonia leaf has the advantages of being simple in method, convenient to operate, large in the acquiring base of the cutting slips, fast in adjustment, high in efficiency and realizable in 75 % rooting rate.

The invention discloses a method for cultivating and planting potted chaenomeles cathayensis, and relates to the technical field of chaenomeles cathayensis cultivation. The planting method comprises the following steps that 1, nutrient soil is prepared; 2, filling of potting soil is carried out, wherein two staggered stacked tiles are arranged at a bottom hole of a flowerpot, a river sand layer, anutrient medium layer and a nutrient soil layer are sequentially laid, and a pH adjusting solution is sprayed to adjust the pH value of the nutrient soil to be 7-9; 3, plants are pruned, wherein sidebranches of the chaenomeles cathayensis plants to be placed in the flowerpot are pruned; 4, the plants are placed in the flowerpot, wherein after leaves of the plants fall in autumn, the chaenomelescathayensis plants are placed into the flowerpot; 5, early-stage management is carried out; 6, pruning and shaping are performed; 7, soil in the flowerpot is turned over, wherein from March to April in the next year, the soil layer on the roots of the plants is loosened; 8, management is carried out in the flowering phase; 9, the plants are management. According to the method, the soil in the flowerpot is turned over while the plants are put into the flowerpot in autumn, and the problem that a manufacturing and cultivation period of existing potted chaenomeles cathayensis is long is solved.

A hybrid begonia, designated 1600-05T1, is disclosed. The invention relates to the seeds of hybrid begonia 1600-05T1, to the plants of hybrid begonia 1600-05T1 and to methods for producing a begonia plant by crossing the cultivar 1600-05T1 with itself or another begonia. The invention further relates to methods for producing a begonia plant containing in its genetic material one or more transgenes and to the transgenic begonia plants and plant parts produced by those methods and to methods for producing other hybrid begonia derived from hybrid begonia 1600-05T1.

The invention discloses a method for improving blooming quality of chaenomeles x superba, and relates to the technical field of regulation and control of plant blooming. The method comprises the following steps: (1) choosing a kinetin with an appropriate concentration and spraying the kinetin to leaf surfaces of chaenomeles x superba seedling trees in a flower-bud differentiation phase; (2) timely performing weeding and controlling rust diseases for the seedling trees of chaenomeles x superba in a growth phase, and irrigating the seedling trees with anti-freezing water in a flower induction phase, wherein it is better to control the water to be frozen in night and thawed in daylight; and (3) during the flower induction phase of the chaenomeles x superba, the potted chaenomeles x superba seedling trees are put in an artificial climate room for temperature-increased culture. The method can help to increase the blooming rate, the total blooming quantity and the flower forming quantity on inflorescences of the chaenomeles x superba, and effectively prevent the phenomenon that flower buds do not bloom. Also, the method is capable of reducing the color difference between the inner loop and the outer loop on petals and improving the flower color saturation degree, solving the flower color deterioration problem, and has good promotion and application values by improving the blooming quality of the chaenomeles x superba and improving the utilization rate of the chaenomeles x superba flower resources.

The invention discloses a preparation method of malus prunifolia fruit wine. The preparation method comprises the following steps: adding pure water and white sugar into malus prunifolia after seedingthe malus prunifolia by a seed remover, uniformly stirring for pulping, and inoculating active dry yeast for pre-fermentation to obtain malus prunifolia pulp; washing young leaves of fresh cephalanoplos seyetum, then steaming for water-removing, grinding the cephalanoplos seyetum after water-removing and honey, filtering to remove filter residues and obtain cephalanoplos seyetum grinding liquid,performing enzymolysis on the cephalanoplos seyetum grinding liquid, and pulping with pure water to obtain cephalanoplos seyetum serous liquid; mixing the malus prunifolia pulp and the cephalanoplos seyetum serous liquid, fermenting, and adding citric acid to form the malus prunifolia fruit wine. According to the invention, the malus prunifolia is combined with the cephalanoplos seyetum, so that the obtained malus prunifolia fruit wine is high in calcium content, and the acidity of the malus prunifolia is covered.

Begonia 'Guiyun' is obtained by selecting and cultivating F1-generation population obtained by taking B. cirrosa as a female parent and B. guangxiensis as a male parent for artificial pollination hybridization. The Begonia 'Guiyun' is characterized in that asexual reproduction like leaf cutting is adopted to maintain specificity and excellent properties of Begonia 'Guiyun', dichasium is four rounds, number of blooming flowers is large, peduncle is long, leaves are large, oval shape is wide, and curly long pubescence is sparse; Begonia 'Guiyun' has excellent properties like pink tepals, flower diameter of 4-5cm, flowering from December to March of the next year and high cultivation adaptability like resistance to low temperature like -4 DEG C; a matched nutritional matrix of humus soil: perlite: fiber like sphagna: PK fertilizer according to a ratio of 40:3:3:0.5 is used for cultivation, and Begonia 'Guiyun' grows robustly under cultivation conditions that average room temperature is 20 DEG C, average air relative humidity is 50% and illumination intensity is 15000-20000Lx. Oblique illumination is properly increased in the flowering period, and phosphorus-potassium fertilizer is additionally applied, so that Begonia 'Guiyun' is large in flower number and big in flower size and bright in flower color.

The invention relates to a begonia semperflorens sprouting and tending method. The begonia semperflorens sprouting and tending method comprises the following steps that 1, a matrix is selected, wherein the matrix contains peat and perlite, and the volume ratio of the peat to the perlite is 8 to 2; 2, a seedling tray is filled with the matrix in a seedling tray sowing mode; 3, begonia semperflorens seeds are scattered in the seedling tray in the step 2; 4, the seedling tray in the step 3 is put in a germination chamber, the temperature of the germination chamber is adjusted to be 20-26 DEG C, the humidity is adjusted to be 85-95%, the illumination intensity is 2000-4000 lux, a fertilizer is applied one time every other day till the diameter of a first true leaf growing out of each begonia semperflorens seedling is up to 1 cm; 5, the begonia semperflorens seedlings in the step 4 are transplanted to a production greenhouse and are hardened for 4-5 days, wherein the illumination intensity of the production greenhouse is not higher than 10000 lux; 6, the begonia semperflorens seedlings in the step 5 are transplanted to a holed tray, and large and small seedlings are graded.

A hybrid begonia, designated 1600-08T1, is disclosed. The invention relates to the seeds of hybrid begonia 1600-08T1, to the plants of hybrid begonia 1600-08T1 and to methods for producing a begonia plant by crossing the cultivar 1600-08T1 with itself or another begonia. The invention further relates to methods for producing a begonia plant containing in its genetic material one or more transgenes and to the transgenic begonia plants and plant parts produced by those methods and to methods for producing other hybrid begonia derived from hybrid begonia 1600-08T1.

The invention discloses a method for planting malus halliana koehne. The method is characterized by comprising the following steps of step 1, preparation, wherein firstly, the trunk diameter of a sapling of malus halliana koehne is determined, then the diameter of a soil ball is determined according to the trunk diameter of the sapling, step 2, transplantation, wherein according to the diameter ofthe soil ball, the root system of malus halliana koehne is dug up, step 3, cultivation, wherein watering is carried out in a soil pit to keep the soil moist, and the diameter of the soil pit is 1.5 times of the volume of the soil ball; then malus halliana koehne and the soil ball are put into the soil pit, step 4, post transplantation, wherein 6-10 kilograms of humic fertilizers are applied within the 1.5-meter range of the circumference of each plant, step 5, governance, water drainage is carried out in time in the plum rain season to discharge hydrops to prevent root rot; and after the rustdisease occurs, a rust disease agentia is sprayed in time. The method for planting malus halliana koehne has the advantages that the growth speed of malus halliana koehne can be improved, in the cultivation process, the formula agentia is used for removing the rust disease, the survival rate of the plant is improved, the integrity of the appearance of malus halliana koehne is ensured, and the quality of the plant is improved.

The present invention relates to a production technology of eurotium cristatum malus hupehensis tea. A PDA potato culture medium is bottled; the culture medium is inoculated with 4-6 eurotium cristatum blocks; the eurotium cristatum is subjected to static culture and shaker culture; and an eurotium cristatum liquid strain is manufactured; malus hupehensis leaves are washed cleaned; the washed malus hupehensis leaves are dried or sun-dried; black tea is soaked using boiling water; the soaked black tea is filtered to obtain a filtrate; the malus hupehensis leaves are soaked in the black tea filtrate; the soaked malus hupehensis leaves are taken out; the taken out malus hupehensis leaves are packaged using thin polypropylene film bags; the packaged malus hupehensis leaves are sterilized; themalus hupehensis leaves are inoculated with the eurotium cristatum liquid strain; the eurotium cristatum liquid strain is cultured and fermented until the eurotium cristatum grows fully on inside andoutside the malus hupehensis leaf culture medium; the cultured and fermented malus hupehensis leaves are baked; and the baked malus hupehensis leaves are packaged to obtain the eurotium cristatum malus hupehensis tea. Specific malus hupehensis leaves in Shennongjia are used as a raw material; the treated malus hupehensis tea is inoculated with the eurotium cristatum for fermentation; the fermentation by the eurotium cristatum can degrade tannin, alkaloid, etc., and starch; and the obtained eurotium cristatum malus hupehensis tea has a brownish red color after brewing, is free of crude green taste of traditional malus hupehensis tea and sweet in taste, and has effects of promoting digestion, lowering blood lipid, dissolving fat, regulating sugar metabolism, etc.

The invention discloses a tissue culturing method of begonia semperflorens. The method comprises the following steps: (1) sterilization: selecting an explant of the begonia semperflorens; washing theexplant of the begonia semperflorens with tap water, then soaking the explant with alcohol, then cleaning the explant with sterile water, and then sterilizing the explant with sodium hypochlorite, washing the explant with sterile water for the second time, and cutting and inoculating the explant of the begonia semperflorens; (2) soaking by nutrient solution, preparing a 6-BA nutrient solution of 1 / 2MS+9.0mg / L, soaking the explant with the 6-BA nutrient solution for 1 hour at 25 DEG C; (3) tissue culturing of leaf blades: putting the explant soaked with the nutrient solution in the step (2) into culture media having different hormones and proportions, and sequentially inducing the explant to form wound callus, inducing the wound callus to generate buds, inducing the buds to carry out proliferation and differentiation, and inducing the buds to carry out rooting so as to obtain adult seedlings; (4) seedling exercising and transplantation: putting the adult seedlings obtained in the step (3) into a culture bottle, opening a lid of the culture bottle and carrying out seedling exercising for 2 days in a room, taking out the adult seedlings and cleaning a base part culture medium, and transplanting into a matrix in which the proportion of perlite to humic matter is 1 to 2.

The present invention discloses a nutrition liquid for adam begonia. The nutrition liquid comprises a catalysis nutrition liquid and an adjustment nutrition liquid. According to the present invention, with the sufficient considering of the sunshine, the rainfall amount, the soil components and other factors of the planting place, the nutrition liquid suitable for the local is prepared, such that it is ensured that the growth condition of the adam begonia is similar, the color is similar, and the size and the height are relatively uniform when the adam begonia is subjected to large-scale cultivation and breeding so as to provide a large number of the high-quality adam begonia for viewing.

The invention discloses a malus hupehensis leaf and hippophae rhamnoides functional food and a preparation method thereof. The malus hupehensis leaf and hippophae rhamnoides functional food is prepared from the raw materials in percentage by mass: 20-80% of malus hupehensis leaves and 20-80% of hippophae rhamnoides. The preparation method of the malus hupehensis leaf and hippophae rhamnoides functional food comprises the steps: the malus hupehensis leaves and the hippophae rhamnoides are pretreated and then added with ethyl alcohol to be subjected to reflux extraction, obtained filtrates are combined, through ethyl alcohol recovery, extract hot water dispersing, still standing, centrifuging, precipitate vacuum drying, maltodextrin adding, soft material preparation, pelletizing, drying andpellet homogenizing, total pellets are obtained, and then the malus hupehensis leaf and hippophae rhamnoides functional food is obtained. A study finds that after all the raw materials are combined tobe used, the malus hupehensis leaf and hippophae rhamnoides functional food plays a synergistic interaction effect and has remarkable effects of lowering blood glucose and blood fat.

The invention discloses a tissue culture rapid-propagation method of a fire axillary bud of a North American crabapple tableland. The tissue culture rapid-propagation method comprises the following steps: taking an axillary bud on a spring twig of a fire crabapple of the North American crabapple tableland as an explant, disinfecting and inoculating, then culturing on an initial culture medium till the axillary bud is germinated, and then transferring the explant to a proliferation culture medium for proliferation culture; when a split bud grows to 2-3 cm, transferring to a rooting culture medium for rooting induction to form a complete plant; carrying out seedling adaptation, domestication and transplantation survival on a plantlet to obtain an expanding propagation seedling. The tissue culture rapid-propagation method disclosed by the invention can be used for inducing the axillary bud of the fire crabapple of the North American crabapple tableland by adopting a tissue culture technology and establishing an in-vitro rapid propagation technical system of an axillary bud regenerated plant, thereby keeping the excellent variety and providing the seedlings on a large scale; in addition, the tissue culture rapid-propagation method disclosed by the invention achieves the proliferation rate of more than 340%, the plantlet rooting rate of more than 65% and the survival rate of more than 80%, can be used for accelerating the propagation speed and scale of the fire North American crabapple and has very good practicability.

The invention relates to a method for prolonging preservation time of Malus prunifolia. The method comprises specific steps as follows: (1) fruit treatment; (2) storage method; (3) daily management. Experiments prove that the preservation time of the Malus prunifolia is effectively prolonged with the method for prolonging the preservation time of the Malus prunifolia, the average quality guaranteed period of the Malus prunifolia is 42 days with the prior art, and the quality guarantee period of the Malus prunifolia is prolonged to 55 days or longer. The risk of rotting caused by poor sales of the Malus prunifolia is effectively reduced, and huge economic benefits can be brought to planting farmers and distributors.