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A kind of tissue culture method of North American crabapple

A technology of tissue culture and crabapple, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of low survival rate of cuttings, achieve the effects of easy reproduction, expanded planting area, and accelerated reproduction speed

Active Publication Date: 2015-12-23
中林绿苑江苏生态环境有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Purpose of the invention: The technical problem to be solved by the present invention is to provide a tissue culture method of North American crabapple, which accelerates the propagation speed of North American crabapple by adopting the method of tissue culture, and solves the problem of low survival rate of adopting cuttings

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0026] A method for tissue culture of North American crabapple, the specific operation steps of the method are:

[0027] one. Primary culture:

[0028] (1) Pretreatment of explants

[0029] Cut off the branches of North American Begonia that were born in the same year, put the harvested branches into the growth room, and promote the germination of leaf buds to become young shoots. When the young shoots grow to 4 cm, they are cut off as explants. Implants were soaked in bleach supernatant for 4 minutes, then rinsed with running water for 20 minutes;

[0030] (2) Sterilization of explants

[0031] After washing the explants with running water, drain the water, soak them in 70% alcohol solution for 15 seconds, then soak them in 1% sodium hypochlorite solution for 5 minutes, rinse them repeatedly with treated sterile water for 3 times, and use sterile absorbent paper Drain moisture for later use;

[0032] (3) Inoculation and culture

[0033] The explants are sterilized and c...

Embodiment 2

[0042] A method for tissue culture of North American crabapple, the specific operation steps of the method are:

[0043] one. Primary culture

[0044] (1) Pretreatment of explants

[0045] Cut off the branches of North American Begonia that were born in the same year, put the harvested branches into the growth room, and promote the leaf buds to germinate into young shoots. When the young shoots grow to 6 cm, they are cut off as explants. Implants were soaked in bleach supernatant for 5 minutes, then rinsed with running water for 30 minutes;

[0046] (2) Sterilization of explants

[0047] After washing the explants with running water, drain the water, soak them in 70% alcohol solution for 15 seconds, then soak them in 1% sodium hypochlorite solution for 8 minutes, rinse them repeatedly with treated sterile water for 5 times, and use sterile absorbent paper Drain moisture for later use;

[0048] (3) Inoculation and culture

[0049] The explants are sterilized and cut into ...

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Abstract

The invention discloses a tissue culture method of North American begonia. The tissue culture method comprises the steps of primary culture, subculture, rooting culture and acclimatization for transplant, wherein current-grown tender branches of the North American begonia are taken as explants and are cultured on a primary culture medium for a period of time through sterile inoculation, then the seedlings of the North American begonia on the primary culture medium are cut and then inoculated on a subculture medium for subculture, clustered seedlings growing to be 2cm-3cm are transferred to a rooting culture medium for rooting induction so as to form rooting seedlings, and the rooting seedlings are subjected to hardening and acclimatization to obtain regeneration plants. According to the method, the North American begonia can be bred more easily, a large quantity of sterile seedlings can be obtained in a short time, and the breeding cycle is shortened, so that the planting area can be enlarged rapidly.

Description

technical field [0001] The invention relates to a plant tissue culture method, in particular to a North American crabapple tissue culture method. Background technique [0002] North American crabapple belongs to the Rosaceae apple genus, with high ornamental value, rich in flower color, leaf color, fruit color and branch color. In addition, the landscape highlighted by its flowers, leaves, fruits, branches and various forms in different seasons makes the viewing period last throughout the year. Therefore, North American crabapple has become a group of characteristic products in garden plant materials under northern climate conditions. [0003] There are many cultivation methods for Begonias in North America, among which the cutting method is the most simple and convenient, but cutting has higher requirements on climate and soil, the season of cutting should be carried out when the temperature is slightly higher in spring, and the temperature is stable, and the freezing weath...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 史清云史云光王海珍张荣良蔡亚萍朱艳
Owner 中林绿苑江苏生态环境有限公司
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