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Tissue Culture Rapid Propagation Method of Jinjili Begonia

A tissue culture and rapid technology, applied in the field of plant tissue culture, can solve the problems of long propagation cycle of Jinjili crabapple seeds, inability to carry out large-scale propagation, and restrictions on the promotion and application of varieties, and achieve fast propagation speed, high rooting rate, and robust growth Effect

Inactive Publication Date: 2016-01-20
武汉市林业果树科学研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Jinjili crabapple seeds have a long propagation cycle and poor resistance. Now most of them are propagated by grafting. However, due to the limitation of the number of scions, large-scale propagation cannot be carried out, which limits the popularization and application of this variety.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for fast propagation of Jinjili Begonia tissue culture, comprising the following steps:

[0023] 1) Selection and cleaning of explants: From April to May, the current year’s branches containing axillary buds were collected from the strong branches of the mother plant of Jinjili Begonia, soaked in soapy water for 5-15 minutes, rinsed with running water for 30-90 minutes, and then set aside;

[0024] 2) Disinfection of explants: Disinfect the cleaned explants with alcohol with a volume fraction of 65-75% on the ultra-clean workbench for 30-60 seconds, rinse with sterile water 3-5 times, each time for 2-4 minutes ; Disinfect with 0.2-0.3% mercury liter for 5-10 minutes, rinse with sterile water 3-5 times, 2-4 minutes each time;

[0025] 3) Primary culture: cut the sterilized explants into 2-4 cm long stems containing axillary buds on the ultra-clean workbench, and insert the stems into the bud induction medium at a culture temperature of 25 ± 1. The light time at...

Embodiment 2

[0029] The Jinjili Begonia tissue culture rapid propagation method comprises the following steps:

[0030] 1) Selection and cleaning of explants: From April to May, the current year's branches containing axillary buds were collected from the strong branches of the mother plant of Jinjili Begonia, soaked in soapy water for 10 minutes, rinsed with running water for 60 minutes, and then set aside;

[0031] 2) Disinfection of explants: Disinfect the cleaned explants with 70% alcohol on the ultra-clean workbench for 30 seconds, rinse with sterile water 4 times, each time for 2 minutes; then disinfect with 0.2% mercury liter for 8 minutes, without Rinse with bacterial water 5 times, 2 minutes each time;

[0032] 3) Primary culture: Cut the sterilized explants into 2cm-long stem segments containing 1 axillary bud on an ultra-clean workbench, and insert the stem segments into bud induction medium at a culture temperature of 25± 1. The light time at ℃ is 15h, and the light intensity i...

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PUM

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Abstract

The invention relates to a tissue culture and rapid propagation method jinjili crabapple. Jinjili crabapple can be obtained through explant selection, explant cleaning, explant disinfection, primary culture, subculture and rooting culture. The tissue culture seedlings of jinjili crabapple obtained by the tissue culture method disclosed by the invention are high in rooting rate and survival speed; the propagation speed is high, the propagation coefficient is more than 5.3, and the rooting rate is over 90 percent; the obtained jinjili crabapple grows well and has a wide market prospect.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a rapid propagation method of Jinjili crabapple tissue culture. Background technique [0002] Jinjili Begonia is a small apple of the genus Rosaceae introduced from North America, and its parents are unknown. Small deciduous tree with glossy leaves. Young leaves are purple in spring and then turn green. The flowers are bright red in large quantities and grow in clusters. It blooms from late March to early April in Wuhan area. The flowering period is as long as two weeks. The young fruit is dark red and turns bright red later. It can be hung until January of the next year; it can be used as a street tree, and it is also one of the fine tree species in the fruit viewing bonsai. It is an excellent tree species for viewing flowers, leaves, and fruits. [0003] Jinjili crabapple seeds have a long propagation cycle and poor resistance. Now most of them are propagated by ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 李长林杨守坤金莉宿福园姚延兴刘先葆裴忺
Owner 武汉市林业果树科学研究所
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