The Tissue Culture Method of Terri Begonia

Abstract

The invention discloses a tissue culture method for Terri crabapple. The tissue culture method comprises the steps of explant selection, primary culturing, subculturing, rooting culturing and transplanting. Washed and disinfected current-year Terri crabapple branches serve as explants, a bud inducing culture medium is inoculated with the explants, primary culturing is carried out, then, an enrichment culture medium is inoculated with the explants so that the explants can be subcultured, next, a rooting culture medium is inoculated with the explants so that rooting culturing can be carried out, and finally, rooted seedlings are transplanted to obtain regeneration plants. By means of the method, the rooting rate, the survival rate and the reproductive speed are high, the obtained Terri crabapple grows robustly, and the tissue culture method has wide market prospects.

Description

technical field [0001] The invention belongs to a plant propagation method, in particular to a tissue culture method of Terri Begonia. Background technique [0002] Terri Begonia, also known as auspicious fruit, red century, red lantern, Yangdonghong, golden century, etc., is a small apple of the Rosaceae family introduced from North America. The female parent is Shandingzi, and the male parent is unknown. Small deciduous tree with smooth bark and glossy leaves; pink to white flowers, large number of flowers, long flowering period, generally more than 20 days; fruit in mid-to-late April, ripe fruit in mid-to-late October, red skin, neat and uniform, The young fruit is green and turns red in the later stage. The fruit is large and arranged in sequence on the branches. It has strong fruiting ability year after year and a long fruit-hanging period. After treatment, it can hang until January of the next year. This variety has strong heat resistance, cold resistance, drought res...

AI Technical Summary

Problems solved by technology

[0003] Terri Begonia has a long seed propagation cycle and few seeds, and now mostly adopts grafting propagation, but due to the limitation of the season and the number of scions, large-scale propagation cannot be carried out, thus limiting the popularization and application of this variety

Chinese invention patent (authorized announcement number CN 103392597 B authorized announcement date 2015.12.23) discloses a tissue culture method of North American crabapple. Branches are explants, after aseptic inoculation on the primary culture medium for a period of time, the North American crabapple seedlings on the primary culture medium are cut and inoculated on the secondary culture medium for subculture. When the clustered seedlings grow to 2~ At 3cm, transfer to the rooting medium to induce rooting to form rooting seedlings, and the rooting seedlings are domesticated to obtain regenerated plants. The method of this invention makes the reproduction of North American crabapple easier, and a large number of sterile seedlings can be obtained in a short time. The reproduction period is shortened, but in this invention, NAA (1-naphthalene acetic acid) is added in each medium, and NAA is toxic to the human body, and the clustered seedlings transferred to the rooting medium are 2 to 3 cm, and the clustered seedlings of 2 to 3 cm If it is too small, it will take a long time to grow roots when it is inserted into the rooting medium