A method for tissue culture and rapid propagation of the fire axillary buds of Begonia plateau in North America
A tissue culture rapid propagation and plateau technology, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of inability to carry out large-scale reproduction and limited popularization of cultivation, so as to speed up the propagation speed and scale, and is very practical sexual effect
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Embodiment 1
[0023] A rapid propagation method of plateau fire Begonia tissue culture, comprising the following steps:
[0024] 1) Selection and pretreatment of explants: In April, select tender branches about 5 cm long from the mother plant of Plateau Fire Begonia, soak them in soapy water for 10 minutes, brush off surface stains with a soft brush, rinse them with running water for 2 hours, and set aside .
[0025] 2) Disinfection of explants: Soak the explants in 75% alcohol for 30 seconds on the ultra-clean workbench, rinse them with sterile water for 3 times, and then disinfect them with 0.1% mercury liter for 9 minutes. Bacterial water was washed 4 times, and the surface moisture was blotted dry with sterile filter paper for later use.
[0026] 3) Primary culture: After the explants were sterilized, they were cut into about 2 cm stem segments with one axillary bud, and inoculated on the primary medium for cultivation ( figure 1 ). The temperature of the culture room is 25±2°C, the ...
Embodiment 2
[0032] A rapid propagation method of plateau fire Begonia tissue culture, comprising the following steps:
[0033] 1) Selection and pretreatment of explants: In April, select tender branches about 5 cm long from the mother plant of Plateau Fire Begonia, soak them in soapy water for 10 minutes, brush off surface stains with a soft brush, rinse them with running water for 2 hours, and set aside .
[0034] 2) Disinfection of explants: Soak the explants in 75% alcohol for 30 seconds on the ultra-clean workbench, rinse them with sterile water for 4 times, and then disinfect them with 0.1% mercury liter for 9 minutes. Wash with sterile water for 5 times, and blot the surface moisture with sterile filter paper for later use.
[0035] 3) Primary culture: After the explants were sterilized, they were cut into about 2 cm stem segments with one axillary bud, and inoculated on the primary medium for cultivation ( figure 1 ). The temperature of the culture room is 25±2°C, the light time...
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