The invention discloses a method for promoting early flowering of Dendrobium nobile by combination of low temperature and plant growth regulators. The method comprises the following steps: culturing Dendrobium nobile for 45 days in an alpine greenhouse, keeping day and night temperatures in the alpine greenhouse at 18-31 DEG C and 7-18 DEG C, respectively, and spraying plant growth regulator solution to Dendrobium nobile stems every 10 days during the culture; after alpine greenhouse cultivation, transferring Dendrobium nobile to a greenhouse in a plain, keeping the day and night temperaturesat 25 DEG C and 20 DEG C, respectively, and culturing for 60-70 days till Dendrobium nobile plants flower. The method of the invention can promote flower bud induction and normal flowering for Dendrobium nobile, significantly increase normal flowering rate of plants, normal flowering rate of nodes and average number of flowers per node, thus greatly improving the viewing quality and commercial value of Dendrobium nobile. Compared with alpine flower induction method in prior arts, the method can achieve a better flower induction effect in the aspects of normal flowering rate, flower number perplant and the like, flower quality has been markedly improved, and no harm is caused by the use of plant growth regulators.