Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for inducing flowering of caryophyllaceae plant

A technology of Caryophyllaceae and plants, which is applied in the field of inducing flowering of Caryophyllaceae plants, and can solve the problems of difficult to obtain sterile pollen, no pollen, and difficult to obtain effective pollen of Caryophyllaceae

Active Publication Date: 2018-06-29
CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Under natural conditions, sterile pollen of Dianthus is difficult to obtain, which makes it difficult to create ploidy materials of Dianthus
There are reports of flowering of Dianthus under in vitro culture conditions, but there is no pollen or it is difficult to obtain effective pollen with germination vigor

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for inducing flowering of caryophyllaceae plant
  • Method for inducing flowering of caryophyllaceae plant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] (1) Medium preparation

[0069] Prepared with 0.1-0.3mg / L paclobutrazol and 0.1–1mg / L NH 4 NO 3 MS medium; preparation containing 0.1-0.5mg / LZnSO 4 and 0.5–1mg / L H 3 BO 3 1 / 2MS medium; at the same time, prepare 0.1-0.5mg / L ZnSO 4 , 0.5–1mg / L H 3 BO 3 and 0.1–0.3 mg / L IBA (indole butyric acid) in MS medium.

[0070] (2) Cultivate sprouts in the vegetative growth period

[0071] Inoculate the aseptic sprouts of the new carnation variety Yuanhong (obtained from the Shanghai Institute of Biological Sciences, Chinese Academy of Sciences) in MS medium for 2 weeks, and then inoculate the acquired Yuanhong sprouts in MS+0.1-0.3mg / L paclobutrazol+ 0.1–1mg / L NH 4 NO 3 Culture medium for 2-4 weeks.

[0072] (3) Cultivate buds in transition period of reproductive growth

[0073] The Yuanhong sprouts obtained in step (2) were inoculated in 1 / 4MS medium and cultivated for 2 weeks, and then the Yuanhong sprouts obtained were inoculated in 1 / 2MS+0.1-0.5mg / L ZnSO 4 +0.5–1mg...

Embodiment 2

[0084] The steps of this embodiment are basically the same as in Embodiment 1, except that Chinese carnation is used.

[0085] The method of the invention can obtain the flowering carnation and the aseptic pollen with germination activity, and the proportion of the aseptic pollen with germination activity is 80-95%, and the flowering rate is 90%.

[0086] Using conventional methods, the proportion of pollen with germination activity in Dianthus chinensis is very low, close to zero.

[0087] Therefore, the Chinese carnation cultivated by the method of the present invention has a very high flowering rate and a sterile pollen rate with germination vigor.

Embodiment 3

[0089] The steps of this embodiment are basically the same as that of Embodiment 1, except that colorful carnations are used.

[0090] Flowering carnation and pollen with germination activity can be obtained by using the method of the invention, and the proportion of pollen with germination activity is 80-95%, and the flowering rate is 90%.

[0091] Using conventional methods, the proportion of germinating pollen in Dianthus variegata is very low, close to zero. Therefore, the carnation cultivated by the method of the present invention has a very high flowering rate and a sterile pollen rate with germination vigor.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for inducing flowering of caryophyllaceae plant. Specifically, the method for inducing flowering of caryophyllaceae plants provided by the invention comprises the following steps: (a) cultivating sterile bud seedlings of caryophyllaceae plant to obtain bud seedlings in the vegetative period; (b) continuously cultivating the bud seedlings in vegetative period obtained in the step (a) so as to obtain bud seedlings in the reproductive growth conversion period; (c) cultivating the bud seedlings in the reproductive growth conversion period obtained in the step (b), and rooting to obtain a flowering plant. According to the method, the sterile bud seedlings of caryophyllaceae plant is firstly taken into an initial explant, in vitro flower induction is carried out to obtain effective sterile pollen, an vitro flower induction system of caryophyllaceae plant is established, and reliable basis is established for the cultivation of haploid double haploid breeding material of caryophyllaceae.

Description

technical field [0001] The invention relates to the field of caryophyllaceae plant cultivation, in particular to a method for inducing flowering of caryophyllaceae plants. Background technique [0002] Dianthus caryophyllus has rich varieties of flowers and colors, fresh and elegant colors, and is deeply loved by consumers at home and abroad. Especially Mother's Day is used frequently. Therefore, the creation of new germplasm of carnation has good market prospects and economic benefits. [0003] Over the years, carnation, carnation, and Chinese carnation are usually used as starting materials to carry out research on the innovation and creation of germplasm resources of carnation, in order to solve the problems of petal weight (flower base number), flower color, plant height, Resistance and asexual reproduction species degradation and other issues. [0004] Cross-breeding is the most common method used to breed new varieties of Dianthus. For example, Israel's largest car...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/005
Inventor 朱木兰刘航池坚张冬菊卫志明
Owner CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com