Establishment method of rosachinensis micro-propagation system

A method for establishing a rose, a technology for rose, which is applied in the field of plant genetic transformation, can solve the problems of low survival rate, low rooting rate, and low flower formation rate of a rose regeneration system, and achieves high survival rate, high rooting rate, and high flower formation rate. Effect

Inactive Publication Date: 2017-05-10
JILIN NORMAL UNIV
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Problems solved by technology

[0004] The purpose of the present invention is to apply plant tissue culture technology, on the basis of the prior art, to provide a more perfect and complete method for establishing a rose micropropagation system, to overcome the problems of low flowering rate, low rooting rate and low survival rate of rose regeneration system question

Method used

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  • Establishment method of rosachinensis micro-propagation system
  • Establishment method of rosachinensis micro-propagation system
  • Establishment method of rosachinensis micro-propagation system

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Embodiment 1

[0030] 1. The configuration of culture medium

[0031] Bud induction medium (MS+TDZ 1.0mg / L+NAA 0.1mg / L+agar 7.2g / L+sucrose 30g / L), pH5.83~5.85;

[0032] Bud proliferation medium (MS+TDZ 1.0mg / L+NAA 0.05mg / L+agar 7.2g / L+sucrose 30g / L), pH5.83~5.85;

[0033] Flower bud induction medium (MS+TDZ 1.0mg / L+agar 7.2g / L+sucrose 40g / L) during flower induction, pH5.83~5.85; the specific formula of bud proliferation medium is (MS+TDZ 1.0mg / L +NAA0.1mg / L+activated carbon 0.6g / L+sucrose 30g / L+agar 7g / L), pH 5.83~5.85;

[0034] Rooting medium (1 / 2MS+activated carbon 1.0g / L+agar 7g / L+sucrose 40g / L), pH 5.83~5.85.

[0035] The 1 / 2 MS medium in the present invention means that the macroelements and trace elements in the normal MS medium are reduced to 1 / 2 of the original, and the specific composition is consistent with the composition of the 1 / 2MS medium recorded in the Chinese patent CN 104115743B .

[0036] The preparation of the MS medium refers to the preparation method of the MS mediu...

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Abstract

The invention discloses an establishment method of a rosachinensis micro-propagation system. The method comprises the following five steps of sterilizing an explant of container rosachinensis, carrying out bud induction culture, carrying out bud propagation culture, carrying out flower induction culture, and carrying out rooting culture, so that the container rosachinensis is successfully cultured in a culture bottle. A culture medium used in a bud induction culture process is MS+TDZ 1.0mg / L + NAA 0.1mg / L +agar 7.2g / L + saccharose 30g / L, and a culture used in a bud propagation culture process is MS+TDZ 1.0mg / L + NAA 0.05mg / L +agar 7.2g / L + saccharose 30g / L. The rosachinensis cultured through the method is higher in flower induction ratio and rooting rate and high in survival rate, has a great economic value, and lays a foundation for follow-up molecular biology study.

Description

technical field [0001] The invention relates to a method for establishing a rose micropropagation system, which belongs to the field of plant genetic transformation, and in particular relates to a method for establishing a common rose micropropagation system. Background technique [0002] Rose (Rosa hybrida) has a history of more than 1,000 years in my country. It is mainly divided into six categories: species-flower rose, strong-flower rose, vine rose, perfume rose, shrub rose and rose. During the three years from 2005 to 2008, a total of 831 rose varieties were registered internationally, including 169 Fenghua roses, 204 hybrid perfume roses, 89 small flower roses, 161 shrub roses, and 106 roses. In foreign countries, rose was introduced to Britain and France in the 17th and 18th centuries. After that, European people used it as an important germplasm to crossbreed with rose, and cultivated many high-quality new varieties. At present, more than 20,000 new rose varieties ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 周晓馥宁思淇武慧
Owner JILIN NORMAL UNIV
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