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Method for Inducing Flowering of Phalaenopsis in Test Tube

A test-tube flowering and phalaenopsis technology is applied in horticultural methods, botanical equipment and methods, horticulture and other directions, and can solve the problems that flower buds are difficult to further develop into flowers, flower buds cannot be opened normally, and phalaenopsis cannot be realized in vitro. The effect of normal and robust flower bud development, lasting flowering period, and simple and easy method

Active Publication Date: 2014-10-29
SUBTROPICAL CROPS INST OF FUJIAN PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And the flower buds cannot open normally in the end (Chiu Y T, Chang C. Induction of in vitro flowering in the Phalaeopsis orchid[J]. J. Taiwan Soc. Hort. Sci., 2011, 57(1): 43-51)
It can be seen from the existing research that it is still impossible to realize the normal test tube flowering of Phalaenopsis at home and abroad.
There is a problem that flower buds are difficult to further develop into flowers

Method used

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  • Method for Inducing Flowering of Phalaenopsis in Test Tube

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Experimental program
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Effect test

Embodiment 1

[0016] The strong bottle seedlings of Phalaenopsis rooted by strong seedlings are used as induction materials for induction, which is carried out in two stages, one is the flower bud induction stage, and the other is the flowering cultivation stage.

[0017] (1) Flower bud induction stage: Take the strong bottle seedlings after the rooting of the strong seedlings as explants, and perform flower bud induction after removing the roots. A total of 15 strains were inoculated. The flower bud induction medium is: MS medium + 6-BA5mg / L, and 20g / L sucrose, 3g / L peptone, 150ml / L coconut milk, 0.5g / L activated carbon, 6g / L agar, pH5. 8. And adjust the amount of N and P in the MS medium, N is reduced to 1 / 5 of the original, and P is increased to 2 times of the original. The culture temperature was 18°C. The light time is 12h, and the light intensity is 30μmol m -2 the s -1 . Induced for 3 and a half months.

[0018] (2) Flowering cultivation stage: After the flower bud induction i...

Embodiment 2

[0021] Take the Phalaenopsis strong bottle seedlings that have taken root from the strong seedlings as the induction material and carry out the following steps to induce.

[0022] (1) Flower bud induction stage: Take the strong bottle seedlings after the rooting of the strong seedlings as explants, and perform flower bud induction after removing the roots. A total of 15 strains were inoculated. The flower bud induction medium is: MS medium + 6-BA7mg / L, and 30g / L sucrose, 4g / L peptone, 120ml / L coconut milk, 0.7g / L activated carbon and 7g / L agar, pH5. 7. And adjust the amount of N and P in the MS medium, N is reduced to 1 / 8 of the original, and P is increased to 3 times of the original. The culture temperature was 20°C. Lighting time 16h, light intensity 40μmol m -2 the s -1 . Induction for 4 months.

[0023] (2) Flowering cultivation stage: After the flower bud induction is completed, it can enter the flowering induction stage. The flowering induction medium is: 1 / 2MS m...

Embodiment 3

[0026] Take the Phalaenopsis strong bottle seedlings that have taken root from the strong seedlings as the induction material and carry out the following steps to induce.

[0027] (1) Flower bud induction stage: Take the strong bottle seedlings after the rooting of the strong seedlings as explants, and perform flower bud induction after root removal. A total of 15 strains were inoculated. The flower bud induction medium is: MS medium + 6-BA10mg / L, and 25g / L sucrose, 5g / L peptone, 100ml / L coconut milk, 1.0g / L activated carbon and 8g / L agar, pH5. 6. And adjust the amount of N and P in the MS medium, N is reduced to 1 / 10 of the original, and P is increased to 5 times of the original. The culture temperature was 16°C. Illumination time 16h, light intensity 50μmol m -2 the s -1 . Induction for 3 months.

[0028] (2) Flowering culture stage: the flowering induction medium is: 1 / 2MS medium is used as the basic medium, and sucrose 25g / L, peptone 5g / L, coconut juice 100ml / L, activ...

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Abstract

The invention discloses a method for inducing test tube flowering of phalaenopsis. The method comprises the following step: taking a robust sterile phalaenopsis bottle seedling after rooting of a robust seedling as an explant to perform induction of a flower bud. A flower stalk can be seen after the flower bud is induced by about 3-4 months. Then flowering induction culture is performed, the flower bud can develop normally, a bud appears, the bud develops normally and can bloom normally, and a deformed flower can be avoided. By adopting the method for inducing the flowering of the phalaenopsis, disclosed by the invention, the formation of the phalaenopsis flower bud can be accurately and effectively induced, and the induction rate of the flower bud can achieve 80.0-93.3%. The flower bud can normally develop and successfully induce the flowering in the probability of 60.0-73.3%. The complete flower of a flower organ is as high as 100.0%. The flowering period is lasting, which can be as long as 30-40 days. Generally, the bottle seedling after test tube field planting and rooting needs about 24 months for flowering after transplanting and planting, and the induction of the flowering in the test tube of the bottle seedling only needs 6-8 months.

Description

technical field [0001] The invention relates to a method for inducing flowering of phalaenopsis test tubes, and belongs to the field of flower biotechnology. Background technique [0002] Phalaenopsis hybrida (Phalaenopsis hybrida), a plant of the genus Phalaenopsis (Phalaenopsis) in the family Orchidaceae, is one of the most widely cultivated and popular species in the family Orchidaceae. The flower shape is unique, the colors are bright and diverse, the flowering period is long-lasting, and the ornamental value and economic value are very high. It is known as the "Queen of Orchids". Under greenhouse cultivation conditions, it takes at least 3 years from seed germination to flowering. Among them, the long time required for planting and flowering is one of the important factors restricting hybrid breeding and commercial production. The use of plant biotechnology to induce Phalaenopsis test tube flowering can greatly shorten the growth cycle, and has the advantages of simpl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 曾碧玉
Owner SUBTROPICAL CROPS INST OF FUJIAN PROVINCE
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