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Method for in-vitro rapid propagation of dendrobium officinale

A consistent technology of Dendrobium officinale, which is applied to the field of in vitro flowering of Dendrobium officinale, can solve the problem of low bud induction rate of flowers, and achieve the effects of solving pest and disease infection, shortening breeding years, and solving long flowering cycle.

Active Publication Date: 2016-04-13
CHANGSHA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the Dendrobium plant species that have been successfully induced to flower in vitro include Dendrobium slender stems, Dendrobium nobile, Dendrobium Huoshanense, Dendrobium ChaoPrayaSmile, Den-drobiumSonia17, and DendrobiumMadameThong-In. Bud conduction rate is not high

Method used

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  • Method for in-vitro rapid propagation of dendrobium officinale
  • Method for in-vitro rapid propagation of dendrobium officinale
  • Method for in-vitro rapid propagation of dendrobium officinale

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Utilize the present invention to carry out in vitro flowering induction culture of dendrobium officinale.

[0031] 1) Proliferation induction culture: select aseptic seedlings of Dendrobium officinale with vigorous growth, emerald green leaves and uniform growth and inoculate them into the proliferation medium for conventional induction culture. The proliferation induction medium of Dendrobium candidum is the culture medium with MS solid medium as the basic medium, and the contained sucrose concentration is 30g / L, the concentration of naphthaleneacetic acid is 0.1mg / L, and the concentration of 6-benzylaminopurine is 3.0mg / L. Base, the pH of the medium was 5.8. (Cultivation conditions are the same as step 2). After culturing for 30 days, each Dendrobium officinale seedling can form about 3 clustered buds with a length of 1.5-2 cm.

[0032] 2) Rooting induction culture: select the clustered shoots of Dendrobium officinale in step 1), inoculate them into the rooting medi...

Embodiment 2

[0036] Utilize the present invention to carry out flowering culture in test tube of dendrobium officinale.

[0037] The proliferation-inducing medium is a medium with MS solid medium as the basic medium, and the contained sucrose concentration is 30g / L, the concentration of naphthaleneacetic acid is 0.1mg / L, and the concentration of 6-benzylaminopurine is 3.0mg / L. The pH of the medium was 5.8. Other conditions are with embodiment 1.

[0038] Described rooting induction culture medium is to take 1 / 2MS solid culture medium as basic culture medium, contained sucrose concentration is 30g / L, naphthalene acetic acid concentration is 0.05mg / L, gac concentration is the culture medium of 1.0mg / L, culture medium The pH value is 5.8, and other conditions are the same as in Example 1.

[0039] The flowering induction medium is a medium with MS solid medium as the basic medium, and the contained sucrose concentration is 30g / L, the concentration of naphthaleneacetic acid is 0.1mg / L, and t...

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Abstract

The invention provides a method for in-vitro flowering of dendrobium officinale. The method comprises: propagation culture, rooting culture, flowering culture, and routine culture till flowering. According to the method, the explant disinfection efficiency can reach as high as 90%; the propagation multiple can reach 4; the aseptic seedling rooting rate can reach 100%; the normal flower induction rate can reach a highest value of 84% and a lowest value of 25%; and each plant blossoms with unequal numbers of 1-3 of flowers. According to the method for test-tube in-vitro flowering of dendrobium officinale, the problems that the flowering period of dendrobium officinale in natural and culture conditions is relatively long, and dendrobium officinale easily infects diseases and pest are greatly solved. Dendrobium officinale seedlings can be quickly provided, and the culture cycle is shortened, so that market requirements are met.

Description

technical field [0001] The invention belongs to the field of plant tissue culture, and in particular relates to a method for in vitro flowering of dendrobium officinale. Background technique [0002] Dendrobium officinale (scientific name: Dendrobium officinale Kimura et Migo), also known as: Black knot grass, Yunnan iron bark. Belonging to the order of Micros, it is a perennial epiphytic herbaceous plant of Orchidaceae. Dendrobium officinale is a traditional Chinese medicine in my country and a flower with high ornamental value. In recent years, the Dendrobium candidum industry has developed rapidly, and production has been in a state of short supply. Dendrobium officinale has a long flowering cycle under natural and cultivated conditions, at least 2 years. The in vitro flowering and fruiting technology can not only shorten the breeding period, but also has great significance for clarifying the flowering mechanism and developing the market. At present, the Dendrobium pla...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 陈建荣刘芳唐映红郭清泉
Owner CHANGSHA UNIVERSITY
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