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A kind of method of blooming in test tube of dendrobium candidum

A technology of dendrobium candidum and in-test tube, which is applied in the field of flowering of dendrobium candidum in test tube, can solve the problem of low flower induction bud induction rate, achieve the effect of solving disease and insect pest infection, good quality varieties, and protecting natural resources

Active Publication Date: 2019-05-24
CHANGSHA UNIVERSITY
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  • Application Information

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Problems solved by technology

At present, the species of Dendrobium that have been successfully induced to flower in vitro include Dendrobium nobile, Dendrobium Huoshanense, Dendrobium Chao Praya Smile, Den-drobium Sonia 17, and Dendrobium Madame Thong-In. Although it has been reported, the flower bud induction rate is not high

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  • A kind of method of blooming in test tube of dendrobium candidum
  • A kind of method of blooming in test tube of dendrobium candidum
  • A kind of method of blooming in test tube of dendrobium candidum

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Embodiment 1

[0030] Utilize the present invention to carry out in vitro flowering induction culture of dendrobium officinale.

[0031] 1) Proliferation induction culture: select aseptic seedlings of Dendrobium officinale with vigorous growth, emerald green leaves and uniform growth and inoculate them into the proliferation medium for conventional induction culture. The proliferation induction medium of Dendrobium candidum is the culture medium with MS solid medium as the basic medium, and the contained sucrose concentration is 30g / L, the concentration of naphthaleneacetic acid is 0.1mg / L, and the concentration of 6-benzylaminopurine is 3.0mg / L. Base, the pH of the medium was 5.8. After culturing for 30 days, each Dendrobium officinale seedling can form about 3 clustered buds with a length of 1.5-2 cm.

[0032] 2) Rooting induction culture: select the clustered shoots of Dendrobium officinale in step 1), inoculate them into the rooting medium, and perform conventional induction culture. T...

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Abstract

The invention provides a method for in-vitro flowering of dendrobium officinale. The method comprises: propagation culture, rooting culture, flowering culture, and routine culture till flowering. According to the method, the explant disinfection efficiency can reach as high as 90%; the propagation multiple can reach 4; the aseptic seedling rooting rate can reach 100%; the normal flower induction rate can reach a highest value of 84% and a lowest value of 25%; and each plant blossoms with unequal numbers of 1-3 of flowers. According to the method for test-tube in-vitro flowering of dendrobium officinale, the problems that the flowering period of dendrobium officinale in natural and culture conditions is relatively long, and dendrobium officinale easily infects diseases and pest are greatly solved. Dendrobium officinale seedlings can be quickly provided, and the culture cycle is shortened, so that market requirements are met.

Description

technical field [0001] The invention belongs to the field of plant tissue culture, and in particular relates to a method for in vitro flowering of dendrobium officinale. Background technique [0002] Dendrobium officinale (scientific name: Dendrobium officinale Kimura et Migo), also known as: black knot grass, Yunnan iron bark. Belonging to the order of Micros, it is a perennial epiphytic herbaceous plant of Orchidaceae. Dendrobium officinale is a traditional Chinese medicine in my country and a flower with high ornamental value. In recent years, the Dendrobium candidum industry has developed rapidly, and production has been in a state of short supply. Dendrobium officinale has a long flowering cycle under natural and cultivated conditions, at least 2 years. The in vitro flowering and fruiting technology can not only shorten the breeding period, but also has great significance for clarifying the flowering mechanism and developing the market. At present, the species of Den...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 陈建荣刘芳唐映红郭清泉
Owner CHANGSHA UNIVERSITY
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