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Method for cultivating begonia fangii using water

A technology of Fang's begonia and hydroponics, applied in the field of Fang's begonia hydroponic breeding, can solve the problems of long germination time, slow rooting, long reproduction cycle, etc., and achieve the effect of promoting robust growth, improving survival rate, and improving germination rate.

Active Publication Date: 2016-01-13
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, distilled water or sterile water can be used as the culture medium, and the cut leaves or leaves with petioles are used as the hydroponic propagation method in the absorbent cotton culture dish. The cut leaves or petioles take root slowly, 20 to 25 days, and the number of roots is small, 2 ~3, slender; no germination after rooting, even if it germinates, it takes 30-35 days for a long time to germinate, the number of buds is small, only 3-5, and the germination rate is low, only 30-40%; when transplanting, the root Extending into the absorbent cotton is easy to cause root breakage. Use peat or garden soil as the cultivation substrate. The soil is prone to water accumulation or hardening, causing root rot, and the adventitious root has weak water absorption capacity, photosynthesis is not strong, the survival rate is 60-70%, and the reproduction cycle is long. 60~65 days

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) On June 10, 2015, select healthy and mature leaves, disinfect them with 70% alcohol by volume, and cut the petiole short so that each leaf retains a small petiole or total petiole with a length of 0.5-1.0 cm;

[0019] (2) Spread a layer of 0.3-0.5cm thick absorbent cotton in a sterile petri dish, and spread a layer of sterile qualitative filter paper on it; put the leaves flat on the sterile qualitative filter paper, soak the absorbent cotton with the following rooting culture solution And filter paper: sterile water + 10mg / L vitamin B1 + 10mg / L rooting powder (ABT) solution, and make sure that no water accumulates on the surface of sterile qualitative filter paper; carry out rooting culture under the conditions of temperature 23 ℃ and light intensity 3000lux , cultivated in light for 10 hours a day, cultured in dark for 14 hours, and supplemented the above rooting culture solution every 2 to 3 days, controlled the culture solution fluid within 1mL, kept absorbent co...

Embodiment 2

[0024] (1) Select healthy and mature mature leaves, disinfect with 70% alcohol by volume, and cut short petioles so that each leaf retains a small petiole or total petiole with a length of 0.5-1.0 cm;

[0025] (2) Spread a layer of 0.3-0.5cm thick absorbent cotton in a sterile petri dish, and spread a layer of sterile qualitative filter paper on it; put the leaves flat on the sterile qualitative filter paper, soak the absorbent cotton with the following rooting culture solution And filter paper: 30mg / L vitamin B1+1mg / LNAA+0.3mg / LKT, and make the surface of the sterile qualitative filter paper free of water; carry out rooting culture under the conditions of temperature 23 ℃ and light intensity 5000lux, light culture 10h every day , cultivated in dark for 14 hours, and supplemented the above-mentioned rooting culture solution every 2-3 days, controlled the effusion of the culture solution within 1mL, kept the absorbent cotton and filter paper moist, and 4-7 thick adventitious roo...

Embodiment 3

[0030] (1) On June 26, 2015, select healthy and mature mature leaves, disinfect them with 70% alcohol by volume, and cut the petioles short, so that each leaf retains a small petiole or total petiole with a length of 0.5-1.0 cm;

[0031] (2) Spread a layer of 0.3-0.5cm thick absorbent cotton in a sterile petri dish, and spread a layer of sterile qualitative filter paper on it; put the leaves flat on the sterile qualitative filter paper, soak the absorbent cotton with the following rooting culture solution And filter paper: sterile water + 30mg / L vitamin B1 + 20mg / L rooting powder (ABT) solution, and make the surface of sterile qualitative filter paper free of water; carry out rooting culture under the conditions of temperature 24 ℃ and light intensity 4000lux , cultivated in light for 10 hours a day, cultured in dark for 14 hours, and replenished the above-mentioned rooting culture solution every 2 to 3 days, controlled the culture fluid to less than 1mL, and kept the absorbent...

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Abstract

The invention discloses a method for cultivating begonia fangii using water. The method comprises disinfecting mature leaves, laying a layer of absorbent cotton in an aseptic culture dish, and laying a layer of qualitative filter paper on the absorbent cotton; placing the leaves flatwise on the aseptic qualitative filter paper, soaking the absorbent cotton and the filter paper in a rooting nutrient solution to make the leaves root until 4 to 7 adventitious roots grow up at each petiole; supplementing an indefinite bud induction solution to wet the absorbent cotton and filter paper to make the leaves root until 5 to 10 buds grow up at each rooting portion, supplementing a 1 / 2 MS growth nutrient solution prepared by sterile water to cultivate until small leaves grow up from the germinal bus; and disinfecting the leaves in a shading shed, planting the leaves in a matrix, spraying in the shading shed to raise the humidity in air to obtain begonia fangii. According to the begonia fangii cultivated by the method, the survival rate is 99 to 100%, the germination rate is raised by 40%, the survival rate is raised by 30%, the period is shortened almost one time, so the method has a popularization meaning in breed conservation and production of begonia.

Description

technical field [0001] The invention relates to the technical field of plant asexual propagation, in particular to a hydroponic propagation method of Fang's begonias. Background technique [0002] Fang's Begonia ( Begonia fangii ) compound leaves, leathery leaves, red veins, with high ornamental value. At present, distilled water or sterile water can be used as the culture medium, and the cut leaves or leaves with petioles are used as the hydroponic propagation method in the absorbent cotton culture dish. The cut leaves or petioles take root slowly, 20 to 25 days, and the number of roots is small, 2 ~3, slender; no germination after rooting, even if it germinates, it takes 30-35 days for a long time to germinate, the number of buds is small, only 3-5, and the germination rate is low, only 30-40%; when transplanting, the root Extending into the absorbent cotton is easy to cause root breakage. Use peat or garden soil as the cultivation substrate. The soil is prone to water a...

Claims

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Application Information

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IPC IPC(8): A01G31/00
CPCA01G24/00A01G31/00
Inventor 杜文文崔光芬王祥宁王继华李绅崇段青贾文杰马璐琳李涵
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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