Method for cultivating begonia fangii using water
A technology of Fang's begonia and hydroponics, applied in the field of Fang's begonia hydroponic breeding, can solve the problems of long germination time, slow rooting, long reproduction cycle, etc., and achieve the effect of promoting robust growth, improving survival rate, and improving germination rate.
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Embodiment 1
[0018] (1) On June 10, 2015, select healthy and mature leaves, disinfect them with 70% alcohol by volume, and cut the petiole short so that each leaf retains a small petiole or total petiole with a length of 0.5-1.0 cm;
[0019] (2) Spread a layer of 0.3-0.5cm thick absorbent cotton in a sterile petri dish, and spread a layer of sterile qualitative filter paper on it; put the leaves flat on the sterile qualitative filter paper, soak the absorbent cotton with the following rooting culture solution And filter paper: sterile water + 10mg / L vitamin B1 + 10mg / L rooting powder (ABT) solution, and make sure that no water accumulates on the surface of sterile qualitative filter paper; carry out rooting culture under the conditions of temperature 23 ℃ and light intensity 3000lux , cultivated in light for 10 hours a day, cultured in dark for 14 hours, and supplemented the above rooting culture solution every 2 to 3 days, controlled the culture solution fluid within 1mL, kept absorbent co...
Embodiment 2
[0024] (1) Select healthy and mature mature leaves, disinfect with 70% alcohol by volume, and cut short petioles so that each leaf retains a small petiole or total petiole with a length of 0.5-1.0 cm;
[0025] (2) Spread a layer of 0.3-0.5cm thick absorbent cotton in a sterile petri dish, and spread a layer of sterile qualitative filter paper on it; put the leaves flat on the sterile qualitative filter paper, soak the absorbent cotton with the following rooting culture solution And filter paper: 30mg / L vitamin B1+1mg / LNAA+0.3mg / LKT, and make the surface of the sterile qualitative filter paper free of water; carry out rooting culture under the conditions of temperature 23 ℃ and light intensity 5000lux, light culture 10h every day , cultivated in dark for 14 hours, and supplemented the above-mentioned rooting culture solution every 2-3 days, controlled the effusion of the culture solution within 1mL, kept the absorbent cotton and filter paper moist, and 4-7 thick adventitious roo...
Embodiment 3
[0030] (1) On June 26, 2015, select healthy and mature mature leaves, disinfect them with 70% alcohol by volume, and cut the petioles short, so that each leaf retains a small petiole or total petiole with a length of 0.5-1.0 cm;
[0031] (2) Spread a layer of 0.3-0.5cm thick absorbent cotton in a sterile petri dish, and spread a layer of sterile qualitative filter paper on it; put the leaves flat on the sterile qualitative filter paper, soak the absorbent cotton with the following rooting culture solution And filter paper: sterile water + 30mg / L vitamin B1 + 20mg / L rooting powder (ABT) solution, and make the surface of sterile qualitative filter paper free of water; carry out rooting culture under the conditions of temperature 24 ℃ and light intensity 4000lux , cultivated in light for 10 hours a day, cultured in dark for 14 hours, and replenished the above-mentioned rooting culture solution every 2 to 3 days, controlled the culture fluid to less than 1mL, and kept the absorbent...
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