Tissue culture method of malus hupehensis

A tissue culture and begonia technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of individual differences in sowing and seedling raising, low reproductive efficiency, etc., and achieve easy operation, high transplanting survival rate, and shortening of the breeding cycle. Effect

Inactive Publication Date: 2018-01-30
NANJING FORESTRY UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for tissue culture of Hubei crabapple, which aims to solve the problems of differences among individuals of Hubei c

Method used

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  • Tissue culture method of malus hupehensis
  • Tissue culture method of malus hupehensis

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Embodiment 1

[0023] A method for tissue culture of Hubei crabapple, comprising the following steps:

[0024] S 1 1. Disinfection treatment of explants: at the beginning of March, select robust annual Hubei crabapple seedling semi-lignified single-bud stem sections as explants, first soak them in washing powder solution for 30 minutes, and use a soft brush to scrub the surface dirt, and then After rinsing with running water for 2 hours, place it in an ultra-clean workbench, and use HgCl with a mass concentration of 0.1% 2 The solution was sterilized for 7 minutes, and then rinsed with sterile water for 5 times. After rinsing, the part of the stem in contact with the medicinal solution was cut off, and cut into 1-2 cm stems on sterile filter paper;

[0025] S 2 , primary culture: the step S 1 The treated 1-2cm stem segments were inoculated on the induction medium for primary culture until the axillary buds of the explants germinated, the germination rate was 90.0%, and they grew into 2-3c...

Embodiment 2

[0030] A method for tissue culture of Hubei crabapple, comprising the following steps:

[0031] S 1 1. Disinfection treatment of explants: at the beginning of March, select robust annual Hubei crabapple seedling semi-lignified single-bud stem sections as explants, first soak them in washing powder solution for 30 minutes, and use a soft brush to scrub the surface dirt, and then After rinsing with running water for 2 hours, place it in an ultra-clean workbench, and use HgCl with a mass concentration of 0.1% 2 The solution was sterilized for 7 minutes, and then rinsed with sterile water for 5 times. After rinsing, the part of the stem in contact with the medicinal solution was cut off, and cut into 1-2 cm stems on sterile filter paper;

[0032] S 2 , primary culture: the step S 1 The treated 1-2cm stem segments were inoculated on the induction medium for primary culture until the axillary buds of the explants germinated, the germination rate was 66.7%, and they grew into 2-3c...

Embodiment 3

[0037] A method for tissue culture of Hubei crabapple, comprising the following steps:

[0038] S 1 1. Disinfection treatment of explants: at the beginning of March, select robust annual Hubei crabapple seedling semi-lignified single-bud stem sections as explants, first soak them in washing powder solution for 30 minutes, and use a soft brush to scrub the surface dirt, and then After rinsing with running water for 2 hours, place it in an ultra-clean workbench, and use HgCl with a mass concentration of 0.1% 2 The solution was sterilized for 7 minutes, and then rinsed with sterile water for 5 times. After rinsing, the part of the stem in contact with the medicinal solution was cut off, and cut into 1-2 cm stems on sterile filter paper;

[0039] S 2 , primary culture: the step S 1 The treated 1-2cm stem segments were inoculated on the induction medium for primary culture until the axillary buds of the explants germinated, the germination rate was 80.0%, and they grew into 2-3c...

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Abstract

The invention discloses a tissue culture method of malus hupehensis. The method comprises five steps, namely, the explant disinfecting step, the primary culture step, the subculture step, the rootingculture step and the acclimatization and transplant step. The method has the advantages that the tissue culture rapid propagation technology is utilized; the outstanding characters of an original stock plant remain; the propagated generations are uniform; in addition, the propagation coefficients of the malus hupehensis can be efficiently and quickly increased, and the propagation cycle is reduced; moreover, the transplanting survival rate of the malus hupehensis is high. The method method meets the market demand of southern region on the malus hupehensis and is beneficial protection for the wild resource of the malus hupehensis. In addition, the culture method is easy to operate, requires low labor and time input, and is suitable for market popularization.

Description

technical field [0001] The invention relates to the technical field of plant asexual reproduction, in particular to a method for tissue culture of Hubei crabapple. Background technique [0002] Hubei crabapple (Malus hupehensis) is a plant of Rosaceae (Rosaceae), which is widely distributed in China. It not only has important ornamental value and medicinal and edible value, but also has strong growth, strong resistance and wide adaptability. The area south of the Yellow River Basin in China is often used as an excellent rootstock for the genus Apple. Due to the over-exploitation and utilization of natural forests, the natural community of crabapple in Hubei has been severely damaged, and the stock of wild resources has declined sharply. In 1997, the International Union for Conservation of Nature (IUCN) listed the Hubei crabapple as an endangered species. Therefore, the effective preservation and efficient reproduction of the germplasm resource of Hubei crabapple has become...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 范俊俊张往祥谭芊芊
Owner NANJING FORESTRY UNIV
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