A method for cultivating Camellia japonica seed embryo tissue culture seedlings under sunlight conditions

A Camellia japonica, sunlight technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., to achieve lush growth, lower electricity costs, and good genetic stability

Active Publication Date: 2019-07-02
广西壮族自治区南宁良凤江国家森林公园
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no literatures and patents about using sunlight to carry out industrialized seedling cultivation of Camellia japonica seedlings

Method used

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  • A method for cultivating Camellia japonica seed embryo tissue culture seedlings under sunlight conditions
  • A method for cultivating Camellia japonica seed embryo tissue culture seedlings under sunlight conditions
  • A method for cultivating Camellia japonica seed embryo tissue culture seedlings under sunlight conditions

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Experimental program
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Effect test

Embodiment 1

[0034] A method for cultivating camellia camellia tissue-cultured seedlings in sunlight using seeds as explants, a method for cultivating camellia camellia seed embryo tissue-cultured seedlings under sunlight conditions comprises the following steps:

[0035] (1) Disinfection of explants and primary culture of buds: Collect 7-8 mature Camellia japonica fruits with red-green shells in June, wash the dust on the surface of the fruits with tap water, peel off the exocarp, place them on an ultra-clean workbench, and use 75 % alcohol solution for 30 s, washed 3 times with sterile water, HgCl with a mass fraction of 1‰ 2 Soak in the solution for 8 minutes, wash with sterile water 3 times, peel off the endocarp, inoculate in the primary medium, the formula of the primary medium is MS+50 mg / L Na 2 SO 4 +2 mg / L 6-benzylaminoadenine+0.5 mg / L naphthaleneacetic acid+10 mg / L vitamin C+300 mg / L hydrolyzed casein+4.5 g / L agar powder+30 g / L sucrose, pH=5.8 , placed in a culture room where 8...

Embodiment 2

[0040] A method for cultivating camellia camellia seed embryo tissue culture seedlings under sunlight conditions, comprising the following steps:

[0041] (1) Disinfection of explants and primary culture of buds: Collect 7-8 mature Camellia japonica fruits with red-green shells in June, wash the dust on the surface of the fruits with tap water, peel off the exocarp, place them on an ultra-clean workbench, and use 75 % alcohol solution for 30 s, washed 4 times with sterile water, HgCl with a mass fraction of 1‰ 2 Soak in the solution for 8 minutes, wash 4 times with sterile water, peel off the endocarp, inoculate in the primary medium, the formula of the primary medium is MS+50 mg / L Na 2 SO 4 +2 mg / L 6-benzylaminoadenine+0.5 mg / L naphthaleneacetic acid+10 mg / L vitamin C+300 mg / L hydrolyzed casein+4.5 g / L agar powder+30 g / L sucrose, pH=5.8 , placed in a culture room where 80% of the walls are double-glazed windows ( figure 1 ), the cultivation indoor light time was 11-14 h / d...

Embodiment 3

[0046] A method for cultivating camellia camellia seed embryo tissue culture seedlings under sunlight conditions, comprising the following steps:

[0047] (1) Disinfection of explants and primary culture of buds: Collect 7-8 mature Camellia japonica fruits with red-green husks in July, wash the dust on the surface of the fruits with tap water, peel off the exocarp, place them on an ultra-clean workbench, and use 75% Disinfect with alcohol solution for 30 s, wash with sterile water 4 times, HgCl with a mass fraction of 1‰ 2 Soak in the solution for 8 minutes, wash 4 times with sterile water, peel off the endocarp, inoculate in the primary medium, the formula of the primary medium is MS+50 mg / L Na 2 SO 4 +2 mg / L 6-benzylaminoadenine+0.5 mg / L naphthaleneacetic acid+10 mg / L vitamin C+300 mg / L hydrolyzed casein+4.5 g / L agar powder+30 g / L sucrose, pH=5.8 , placed in the culture room where 80% of the wall is double-glazed windows ( figure 1 ), the cultivation indoor light time wa...

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Abstract

The present invention relates to a method for cultivating camellia nitidissima chi embryo tissue culture seedling under a sunlight condition, wherein the method comprises four steps such as explant sterilization and primary culture of buds, secondary proliferation culture of buds, rooting culture of bud, and nursery stock transplanting. According to the present invention, the used light source is derived from sunlight, and by adjusting the illumination intensity, the growth promoting effect of the sunlight on the camellia nitidissima chi tissue culture seedling is completely provided; after the camellia nitidissima chi bud is cultured for 40 d under the sunlight condition, the proliferation coefficient is 9-13 and the camellia nitidissima chi tissue culture seedling rooting rate is up to 90-95% and is increased by more than 20% compared to the rooting rate of the method using the incandescent lamp as the light source; in the same rooting culture medium, the bud root point growth time under the sunlight is 20-25 d, the bud root point growth time under the incandescent lamp is 30-35 d, the bud root point growth time under the sunlight is shortened by 5-10 d compared to the bud root point growth time under the incandescent lamp while the transplanting survival rate is up to more than 95%, such that the requirements of the industrial production of the camellia nitidissima chi nursery stock are achieved.

Description

technical field [0001] The invention relates to the technical field of asexual rapid propagation of plants, in particular to a method for cultivating Camellia japonica seed embryo tissue culture seedlings under sunlight conditions. Background technique [0002] Camellia ( Camellia nittidissima Chi ), belonging to the genus Camellia in the family Theaceae, is the only species with golden petals in the Camellia family, and is a unique traditional flower in my country. It is also one of the eight national first-level key protected plants and enjoys a high reputation both at home and abroad. Camellia chrysalis is hailed as "the queen of the tea clan" and "the giant panda in the plant world" in China, while it is called "the yellow camellia in fantasy" and "the magical oriental magic tea" in foreign countries. Golden camellia is not only of high ornamental value, but also a precious medicinal material, and its medicinal value has also been widely recognized by the society. Gol...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 韦中绵俞建妹刘芳龙敏陆湘云朱友飞孙灿岳黄艳沈遐卢德阳刘莉张世佐廖敏彤秦庆利苏峰
Owner 广西壮族自治区南宁良凤江国家森林公园
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