80 results about "Embryo tissue" patented technology

The invention discloses the specificity marker protein TS / MEDP antibody preparation and the purpose. The molecular biology technology is utilized, and a new gene is cloned in a stomach cancer cell and is named as TS / MDEP. A polyclonal antibody for preventing the TS / MDEP is prepared, and a high specificity antibody is acquired through the antibody purification technology. The expression characteristics of the TS / MDEP gene in a tumor cell line and a tumor cell tissue are verified from the mRNA and protein level, the expression in 14 tumor cell lines including the tumor cell line is determined, the dependance of cell cycle is assumed, the excess expression exists in stomach cancer, breast cancer and cervical cancer tissues, no expression is in the corresponding normal tissues, and the expression exists in an embryo tissue. The high expression of the TS / MDEP in the embryo tissue with exuberant cell proliferation is validated, after growing up, the gene is closed, and the high expression exists in the tumor cells. The TS / MDEP has the seasonal specificity expression to clew the occurrence and the development of the cell cycle control abnormity caused by the high expression of the TS / MDEP. The obtained antibody for preventing the TS / MDEP can be used to monitor the cell cycle advancement and the clinical biological behavior of the gastroenteric tumor.

Methods for the isolation, expansion and storage of a population of stem cells belonging to human dental follicles, called FENC (Follicle-derived Embryonic Neural Crest stem cells,) including: a) Collection of the follicular sack in sterile conditions, digestion and primary culture growth and expansion; b) Optional amplification; c) FACsorting.

A globulin-1 regulatory region is shown, a nucleotide sequence of approximately 3 kb which provides improved seed preferred, and particularly embryo preferred expression in plants. Methods of use are also shown in preferentially expressing a heterologous protein to the embryo tissue of a plant. The sequence is particularly useful in expression of heterologous proteins to the embryo of monocotyledonous plants, particularly cereals, and maize.

The invention relates to a method for evaluating kidney toxicity of compounds through detecting the contents of creatinine in zebra fish tissues. The method is applied to kidney toxicity researches. In the researches that utilize a zebra fish embryo model to study the toxic effect of compounds on the kidney, the creatinine component in embryo tissues is extracted, then the creatinine content is measured through a LC-MS (liquid chromatography-mass spectrum) method, the creatinine content can be taken as a detection index for evaluating the embryo kidney functions, and thus a rapid and precise compound kidney toxicity evaluation system with strong specificity is built.

A globulin-1 regulatory region is shown, a nucleotide sequence of approximately 3 kb which provides improved seed preferred, and particularly embryo preferred expression in plants. Methods of use are also shown in preferentially expressing a heterologous protein to the embryo tissue of a plant. The sequence is particularly useful in expression of heterologous proteins to the embryo of monocotyledonous plants, particularly cereals, and maize.

This invention presents a way to get a lot of GaoYangMao regrow plant through organizing and cultivate. This method is choosing ripped GaoYangMao seeds antisepticising the seeds then cultivate on the advanced cultivation basis, and let it grow to embryo to advanced polarization cultivate basis, let the cell an embryo tissue grow to a whole plant, then plant it into pot, accomplish the whole process. This way is fit for most GaoYangMao regrow. We get the regrow plant frequently with short period in growing, is fit for advanced inheritance.

A soaking-type atmospheric pressure variable method for promoting germination and soakage comprises the following steps: firstly, selecting a proper amount of seeds and a pressure container and pouring water and the seeds into the container, so that the seeds are soaked in the water; secondly, reducing the pressure in the container in an atmospheric-pressure variable manner to extract air inside the container and in the seed coats and the seed cavities, so that the container and the seeds are in a negative-pressure state; and releasing pressure in the container, wherein the seeds can rapidly absorb water in the pressure releasing process and the soakage of the seeds can be achieved when the seeds absorb the water. By adopting the atmospheric-pressure variable manner, the method allows the seeds with coat mechanical disturbance to achieve the rapid soakage in a short time so as to make enzyme inside the seeds work, so that the embryo tissue in the seeds has high metabolic activity and the embryo can be rapidly divided and germinate.

The present invention is as follows: wheat mature embryos of four kinds of winter wheat cultivated variety Beijing 411, Brock and spring wheat S10 jian-6 and spring wheat strong Tianjin No. 5 are taken as explants for callus induction, differentiation and regeneration system optimization for building efficient dedifferentiation regeneration plants and regeneration grain systems; the mature embryos adopt longitudinal cutting processing, a callus induction medium is MS + 2.0mg.L<-1>2,4-D + 200mg. L<-1>CH + 100mg.L<-1>MI + 250mg.L<-1>Glu, a differentiation and plant regeneration culture medium is MS + 10mg.L<-1>ZT + 0.1mg.L<-1>IAA, the differentiation rate is 100%, the seedling rate is 49.33%, a rooting medium is 1 / 2MS, and regeneration grains can be obtained by hardening, field transplanting and management after the transplanting of rooted test-tube plantlets. Compared with reported Beijing 411 regeneration system, the plant regeneration rate in the study is significantly improved. The Beijing 411 mature embryo tissue culture and regeneration system established in the study provides a good acceptor material for subsequent transgenic studies.

A regulatory region is shown, a nucleotide sequence of approximately 3kb which provides improved seed preferred, and particularly embryo preferred expression in plants. Methods of use are also shown in preferentially expressing a heterologous protein to the embryo tissue of a plant. The sequence is particularly useful in expression of heterologous proteins to the embryo of monocotyledonous plants, particularly cereals, and maize.

The invention provides a method for inducing differentiation of neural stem cells, belongs to the field of biotechnology, and in particular relates to a method for inducing differentiation of neural stem cells by an adult stem cell line. The invention provides a method for inducing the differentiation of the neural stem cells, which comprises the following steps of: 1, establishment of the adult stem cell line, namely separating and purifying animal embryo tissues under an aseptic condition to form adult stem cells, and generating different stem cell lines through tens to thousands of subculture; 2, MSCS induction, namely replacing culture solution after 2 days and adding 0.01 to 100Mmol / l monostalotetrahexosylgangliside to induce respectively; 3, morphological observation of SCS and the induced cells; and 4, immunocyte chemical dyeing, namely performing cell immunofluorescent dyeing on the induced cells with a nidogen, an NSE and a GFAP antibody respectively.

The invention discloses a cell production method for an influenza virus vaccine. The cell production method comprises the following steps: preparing of virus-seeds, microcarrier suspension culture of a Vero cell in a bioreactor, breeding of virus liquid for preparing the vaccine, and inactivation and purification of the virus liquid. According to the invention, chick embryo tissue is replaced with cells for culturing and preparing the influenza virus vaccine, so that the problems of chick embryo pollution and exogenous virus pollution to the chick embryo are solved; through the strict control over the raw materials and the culture conditions, that the produced vaccine is pure can be ensured and the safety of the vaccine is ensured; the bioreactor is used for producing the vaccine, so that advantages of high degree of automation, labor saving and simple and stable production processes are achieved; the production cost can be reduced greatly, the raw material supply limitation does not exist, and the production cycle is short; by using the method for producing the vaccine, less environmental pollution and high easiness in treatment are achieved, and the problems of a great amount of generated waste, high difficulty in treatment, biosafety and public health of the conventional chick embryo production method are solved.

The invention relates to an embryonic tissue segmentation method based on a generative adversarial network, and belongs to the technical field of medical image processing. The method comprises the steps of step 1, performing tissue segmentation mask mapping on an embryonic tissue slice image through a U-net network; step 2, making a segmentation network training set; step 3, configuring parametersrequired by network training to obtain a set network; step 4, training the set organization quality identification network by using the manufactured segmentation network training set; step 5, fixingparameters of the organization quality identification network, and training the set U-net network by using the manufactured segmentation network training set in combination with the organization quality identification network; and step 6, taking the embryonic tissue slice image without the marked segmentation result as input, and generating a corresponding mask image. The network relied on by thesegmentation method uses a classification model to supplement loss during training and segmentation, fully utilizes the information of the cell growth state, and improves the accuracy of the segmentation network in the field of embryo tissue segmentation.

The present invention relates to mature embryo tissue cultivating regeneration method of wheatgrass. During seed selection and treatment, plump seed with the lemma and the palea eliminated is first soaked in solution containing 2, 4-D in 1.0-4.0 mg / L for 1.5-3.0 hr and sterilized, and the mature embryo is then inoculated to callus inducing culture medicine with increased AgNO3 solution to culture. The present invention has the advantages of capacity of promoting the generation one callus organ and somatic cell to raise callus generating rate, capacity of promoting the generation of seed bud, and capacity of increasing the number of adventitious bud the explant generates and raising the regeneration frequency of the plant.

The invention discloses a direct-vision induced abortion uterine curettage device. The device comprises a uterine curettage mechanism, an observation mechanism, a circuit, a negative pressure suctionmechanism and an operation rod. A curettage device of the uterine curettage mechanism is arranged at the front ends of the operation rod and a camera and located in the view of the camera. According to the technical scheme that the camera is arranged on the rear portion, the curettage device is completely located in the view of the camera, the whole operation process can be visible, and no observation dead zone exists. Meanwhile, the curettage device cannot have operation dead corners due to shielding generated by the height of a lens module, embryo tissue can be completely removed when implanted at any position of the uterus, especially for the implantation position near the fallopian tube orifice, namely the bottom of the uterus, the curettage device can easily remove the embryo tissue,and the clinical use process is safer and more efficient. A direct-vision induced abortion uterine curettage system comprises the direct-vision induced abortion uterine curettage device, operation canbe carried out under real-time display of a display system, and the induced abortion operation process is very accurate, safe and efficient.

A regulatory region is shown, a nucleotide sequence of approximately 3 kb which provides improved seed preferred, and particularly embryo preferred expression in plants. Methods of use are also shown in preferentially expressing a heterologous protein to the embryo tissue of a plant. The sequence is particularly useful in expression of heterologous proteins to the embryo of monocotyledonous plants, particularly cereals, and maize.

An object of the present invention is to provide a method of gene introduction, which can transform a Hordeum plant at a higher efficiency compared to that in known Agrobacterium methods, and a method of producing a transformed Hordeum plant. The method of the invention includes a step of subjecting an immature embryo tissue of a Hordeum plant to centrifugation treatment and / or pressurization treatment before the inoculation with Agrobacterium, during the coculture step, and / or after the coculture step, and is characterized in that the coculture medium satisfies at least one of a) containing an antiauxin; b) containing a cytokinin; and c) containing a phenoxy auxin in an amount of less than 2 μM and / or a benzoic auxin in an amount of less than 5 μM, or not containing any phenoxy auxin and / or benzoic auxin.

The invention discloses a method for keeping the integrity of embryos in early embryo tissue immunohistochemistry, and belongs to the technical field of animals and organisms. A polyvinylpyrrolidone solution is added to a phosphate buffer solution, paraformaldehyde, Triton X-100 and hydrogen peroxide which are used for the early embryo tissue immunohistochemistry to prevent embryo attachment, so the integrity of the embryos is kept in the whole immunohistochemistry process, and the immunohistochemistry effect is improved. The method mainly aims at in vitro embryos, and the components of a tissue treatment reagent are adjusted on the basis of adjusting routine immunohistochemistry steps, so the immunohistochemistry effect is effectively improved. The method has the advantages of overcomingthe disadvantages in the immunohistochemistry technology, enlargement of the application range, simplicity in operation, and low production cost.

The present invention provides a Davidia involucrata vitro embryo tissue culture method, comprising: soaking fresh Davidia involucrata vitro embryo in a GA3 solution with the concentration of 8-15 mM at 20-30 DEG C for 1.5-2.5 hours; performing cleaning, disinfecting and sterilizing; and then inoculating the Davidia involucrata vitro embryo in a 1 / 2MS culture medium for culturing. The culture medium comprises: 1 / 2MS, 0.3-0.7 mg / L of 6-BA, 2-5 mg / L of IBA, 25-35 g / L of sucrose and 6-7 mg / L of agar. The present invention can break the dormancy of Davidia involucrata vitro embryo and improve the seedling rate of culture of the Davidia involucrata vitro embryo tissue.

The invention relates to a method for screening germination marker metabolites of Picea asperata Mast. somatic embryo based on an NMR technology. The method comprises: drying somatic embryo of Picea asperata Mast. through a filter paper method, separating cotyledon and radicle of non-dry somatic embryo and cotyledon and radicle of dried somatic embryo, carrying out extraction, freeze-drying and redissolution on different somatic embryo tissue metabolites, and carrying out NMR detection, metabolome data processing and screening of labeled metabolites. Through metabolomics study on different tissue samples of the Picea asperata Mast. somatic embryo, a high-efficiency, rapid and reproducible method for pretreatment on metabolomics samples of Picea asperata Mast. somatic embryo, extraction, data processing and differential metabolite screening is built. The method can effectively reveal and evaluate the related metabolic information about the influence of drying treatment on conversion ofmorphological maturation of the Picea asperata Mast. somatic embryo into physiological maturation.

The invention discloses bovine induced expansion pluripotent adult stem cells, a line establishment method and a culture solution, and the bovine induced expansion pluripotent adult stem cells are obtained by transgenosis and induced culture from bovine fibroblast cells and can be stably subcultured in vitro. The stem cell has totipotency, stability and safety, can be differentiated in an in-vivo and out-of-embryo tissue and an out-of-embryo tissue, and can be applied to various life science and medical fields such as breeding and breeding, gene editing, animal cloning, medical models, drug development carriers, induced production of bovine reproductive gametes and the like.

The invention discloses a dry-germ dissociation method applied to wheat mature embryo culture, and provides an embryo taking-out method of grasses. The method comprises the following steps: smashing kernels of the grasses, using a 9-mesh mesh sieve to remove large particles, then using a 30-mesh mesh sieve to remove small particles, and selecting 1-2 mm embryo tissues from particles of which the sizes are within the range of 9-30 meshes. Compared with the conventional embryo scraping method, the embryo taking-out method provided by the invention has the advantages that although difference between wound curing induction and differentiation effect in follow-up tests is insignificant, processes of embryo taking-out, storage and disinfection become convenient and fast, so that the manpower is saved; the method can be applied to the storage of germ plasm resources to save storage space. According to the method, tissue culture and genetic transformation of mature embryos on the grasses are greatly facilitated. In summary, the method has an important application significance.

The invention discloses a carya illinoensis cell embryogenesis method. The method comprises steps of material selection, sterilization, induction culture of test-tube bud seedlings, induction of somatic embryo, multiplication culture, maturation culture and sprouting of the somatic embryo; the culture is carried out in a somatic embryo induction, multiplication, maturation and sprouting culture medium. The method has high multiplication coefficient, the multiplication time is not subjected to the limitation of seasons, the harm of used materials on a parent body is small, the trouble of diseases and injurious pests can be avoided, excellent characters of original plants can be maintained, and an important application value in production and scientific research of carya illinoensis seedlings can be realized.

The invention relates to a method for producing an avian influenza vaccine by adopting an MDCK cell line and a product of the avian influenza vaccine. The method comprises the following steps of domesticating adherent MDCK cells into the stably proliferated serum-free full-suspension culture type MDCK cell line, then performing toxin inoculation and proliferation, separating and collecting virus liquid, performing inactivation, and adding an adjuvant to prepare the vaccine. The cell line is used for replacing chick embryo tissue culture to prepare avian influenza virus and the avian influenzavaccine, so that the problem that chick embryos are polluted by exogenous virus can be solved; and by strictly controlling raw materials and culture conditions, the purity of the produced vaccine is ensured, the safety of vaccine is ensured, the method can be applied to large-scale production under safe and economic conditions, and the effective avian influenza vaccine with high purity and ideal industrial yield is obtained.

The invention provides a method for the production of soybean embryogenic callus and somatic embryos. The invention further provides methods of transforming explants that includes generating somatic embryo tissue. The transgenic somatic embryos produced can be used for regenerating stable transgenic plants.

The invention relates to the technical field of plant tissue culture, and discloses a berberis amurensis somatic embryo induction medium, a differentiation medium and a corresponding tissue culture method. According to the tissue culture method, a semi-mature zygotic embryo in a berberis amurensis somatic embryo induction key period is selected as an explant, and casein hydrolysate is added into the culture medium to perform tissue induction on the explant, so that a berberis amurensis embryonic callus is obtained, and the induction rate is high. The callus obtained by differentiation can continuously keep the embryogenic property, and can be further differentiated to induce plant regeneration.

The invention discloses a continuous operation method for extraction and separation of stem cells. The continuous operation method comprises the following steps: crushing and extracting embryo piecesin an extraction device, classifying and separating embryo powder in a separation device, quickly and stably stopping the separation device through a speed reducing mechanism, and taking thoroughly separated reagent tubes. Collected embryo pieces are poured into the arranged extraction device and crushed into powder by a smashing barrel, then centrifugal force is generated through rotation of an extraction barrel, tissue with different components is separated out and isolated and collected, embryo tissue can be conveniently and rapidly extracted, and the working environment is kept dry and comfortable. The tissue and liquid in the reagent tubes are separated through centrifugal force produced through rotation of the separation barrel. The speed reducing mechanism is arranged below the separation barrel, so that the separation device can be stopped quickly and stably in a short time, and a researcher can take the reagent tubes for research in a time-saving manner.

The invention provides an oil-in-water-in-oil type inactivated vaccine for an avian influenza virus and a preparation method thereof. The preparation method comprises the following steps: cultivation of the avian influenza virus, inactivation of the virus, virus concentration, vaccine emulsification and the like. Compared with the prior art, the preparation method provided by the invention has the advantages that a local separated avian influenza virus is adopted and bred by using an MDCK (Madin-Darby canine kidney) cell instead of chicken embryo tissue; the production cost of the vaccine is reduced; the quality is easy to balance and stabilize; the yield and the quality of the vaccine are significantly improved. The vaccine is emulsified by using a homogenizer; the prepared vaccine is of an oil-in-water-in-oil type. Compared with the traditional water-in-oil dosage form, the vaccine is low in viscosity, small in particle size and easy to assimilate. The adjuvants used in the oil-in-water-in-oil type inactivated vaccine do not include any surfactant ingredient in the preparation process of the vaccine; the prepared vaccine is good in safety; the side effect due to the fact that the traditional oil adjuvant is difficult to assimilate is avoided.

A process for extracting the human Cu, Zn superoxide dismutase (hCu, Zn-SOD) gene from human tissue includes such steps as extracting the common RNA from human embryo tissue, and reverse transcription-PCR for amplifying cDNA of hCu, Zn-SOD. Its advantages are simple process, low cost, and high sequence homology up to 99.8%.

A Zea mays regulatory region is shown, which provides improved seed preferred, and particularly embryo preferred expression in plants. Methods of use are also shown in preferentially expressing a heterologous protein to the embryo tissue of a plant. The sequence is particularly useful in expression of heterologous proteins to the embryo of monocotyledonous plants, particularly cereals, and maize.