7 results about "Embryo tissue" patented technology

This disclosure provides methods and compositions for increasing genome editing and site-directed integration events utilizing guided endonucleases and meiotic cell-preferred, egg cell-preferred or embryo tissue-preferred promoters.

The utility model discloses a tissue embryo storage device, which belongs to the technical field of embryo storage and comprises a storage tank with a pick-and-place opening. The placing frame is rotatably mounted in the storage tank, and the placing frame is provided with a placing cylinder; the sealing assembly comprises a sealing piston, and the sealing piston is inserted into the taking and placing opening; according to the utility model, the placing rack is arranged, so that a plurality of test tubes filled with tissue embryos are placed in the placing cylinder one by one, and liquid nitrogen is contained in the storage tank; at the moment, the rotating ring rotates to the position concentric with the taking and placing opening, the sealing piston is inserted into the taking and placing opening, it is ensured that the sealing rubber strip is attached to the taking and placing opening, and the inserting column is inserted into the inserting hole, so that the interior of the storage tank is in a closed low-temperature environment, and the taking and placing opening is only aligned with the barrel opening of each containing barrel; and the number of bacteria or viruses entering the storage tank from the taking and placing opening is reduced, so that the risk that the tissue embryos are infected is reduced.

The invention relates to the field of biomedical materials, in particular to Tibetan sheep embryo type III collagen as well as a preparation method and application of the Tibetan sheep embryo type III collagen. Comprising the following steps: sequentially carrying out cation exchange chromatography purification and gel chromatography purification on an extracting solution which is extracted from a Tibetan sheep embryo and contains type III collagen; in the cation exchange chromatography purification step, a sample is loaded in an acetic acid buffer solution with the pH value of 2.5-3.5 and the concentration of 0.4-0.6 M, an eluent is a sodium chloride solution, and gradient elution is carried out within the eluent concentration range of 0-1 mol / L; in the step of gel chromatography purification, an eluent is a phosphate buffer solution with the pH value of 7.2-7.4, and the concentration of the eluent is 0.01-0.1 M; type III collagen with a complete triple helix structure and high biological activity is prepared from Tibetan sheep embryonic tissue, has the properties of good biocompatibility, good thermal stability, promotion of cell proliferation, adhesion and migration and the like, and has high medical value.

This disclosure provides methods and compositions for increasing genome editing and site-directed integration events utilizing guided endonucleases and meiotic cell-preferred, egg cell-preferred or embryo tissue-preferred promoters.

The application discloses a kind of based on low-field nuclear magnetic resonance's poultry egg gender nondestructive identification method, belong to poultry egg detection technical field, comprising: S1, using low-field nuclear magnetic resonance equipment to carry out echo signal acquisition to poultry egg in turning chick period;S2, using non-negative least square optimization model to combine Tikhonov regular term to the echo signal of collection carries out spectrum inversion, reconstructs the T2 relaxation time spectrum related to poultry egg internal moisture and tissue structure distribution;S3, from T2 relaxation time spectrum, extract the multi-dimensional spectroscopy feature associated with poultry egg gender information, form the high-dimensional feature vector of representing embryo tissue microstructure difference;S4, high-dimensional feature vector is input into integrated classification model, and the output poultry egg gender identification result.The method of the application can be integrated on the automatic conveying line of hatching factory, realize multiple egg position parallel detection, fast non-contact identification and classification output, with high adaptability and engineering practicability.

This utility model belongs to the field of embryo tissue culture technology, specifically relating to an intelligent sterile airflow barrier device for an embryo culture chamber. It includes a culture chamber with a vertical plate on the front and a horizontal plate on the top. The top edge of the vertical plate is fixedly connected to one side of an arc plate, the other side of the arc plate is fixedly connected to one side of the horizontal plate, the other side of the horizontal plate is fixedly connected to one side of an inclined plate, and the other side of the inclined plate is fixedly connected to the back of the culture chamber. This utility model can continuously blow clean the hands of operators when opening and closing the culture chamber door, effectively preventing contaminated air from directly entering the chamber; it can also effectively clean residual contaminants in the chamber cavity caused by eddies or dead corners; and it can save energy and extend the service life of the silent fan.

This application relates to the field of biological tissue processing equipment technology, and discloses a closed-loop, high-efficiency tissue mincing device for extracting primary cells from chicken embryos. The device mainly includes a support frame, a cup body, a cooling box, a shearing assembly, and a sealing assembly. The shearing assembly is driven by magnetic coupling, with an external motor rotating a disk, which in turn drives a magnet at the bottom of the cup body to rotate a rotating shaft and moving blades. This creates an alternating shearing action with stationary blades fixed to the inner wall of the cup body, achieving uniform and efficient physical fragmentation of the chicken embryo tissue. The cooling assembly includes a cooling jacket surrounding the cup body and a circulating cooling system, effectively removing heat generated during the shearing process. The sealing assembly consists of a top cover, a sealing gasket, and a feed pipe with a sealing plug, ensuring a completely sealed operation. This invention solves the problems of significant mechanical damage to cells, easy thermal inactivation of samples, and the risk of contamination during open operation in existing technologies. It has the advantages of good cell morphology preservation, high biosafety, and convenient operation.