13 results about "Embryo tissue" patented technology

The invention relates to the technical field of bioinformatics and embryonic development evaluation, and discloses an embryo sample processing and multi-omics detection method, which comprises the following steps: S1, obtaining an embryo sample and carrying out continuous tissue slicing to form a plurality of slice images with a spatial sequence relationship; s2, performing image registration and three-dimensional space reconstruction on the slice image, and establishing a three-dimensional voxel coordinate system of the embryonic tissue; s3, acquiring multiple omics detection data based on the three-dimensional voxel coordinate system, and mapping the data to corresponding voxel coordinate positions; s4, performing feature dimension reduction and representation embedding on the omics data of each mode, and generating a multi-omics fusion feature vector of each space node; and S5, constructing a space graph structure containing the space nodes and the adjacency relation. A unified embedding mechanism of multi-modal omics data is introduced, dimensionality regularization is performed on high-dimensional features through a nonlinear function, and the embedded representation has semantic consistency among different modals.

This disclosure provides methods and compositions for increasing genome editing and site-directed integration events utilizing guided endonucleases and meiotic cell-preferred, egg cell-preferred or embryo tissue-preferred promoters.

The utility model discloses a tissue embryo storage device, which belongs to the technical field of embryo storage and comprises a storage tank with a pick-and-place opening. The placing frame is rotatably mounted in the storage tank, and the placing frame is provided with a placing cylinder; the sealing assembly comprises a sealing piston, and the sealing piston is inserted into the taking and placing opening; according to the utility model, the placing rack is arranged, so that a plurality of test tubes filled with tissue embryos are placed in the placing cylinder one by one, and liquid nitrogen is contained in the storage tank; at the moment, the rotating ring rotates to the position concentric with the taking and placing opening, the sealing piston is inserted into the taking and placing opening, it is ensured that the sealing rubber strip is attached to the taking and placing opening, and the inserting column is inserted into the inserting hole, so that the interior of the storage tank is in a closed low-temperature environment, and the taking and placing opening is only aligned with the barrel opening of each containing barrel; and the number of bacteria or viruses entering the storage tank from the taking and placing opening is reduced, so that the risk that the tissue embryos are infected is reduced.

The invention relates to the field of biomedical materials, in particular to Tibetan sheep embryo type III collagen as well as a preparation method and application of the Tibetan sheep embryo type III collagen. Comprising the following steps: sequentially carrying out cation exchange chromatography purification and gel chromatography purification on an extracting solution which is extracted from a Tibetan sheep embryo and contains type III collagen; in the cation exchange chromatography purification step, a sample is loaded in an acetic acid buffer solution with the pH value of 2.5-3.5 and the concentration of 0.4-0.6 M, an eluent is a sodium chloride solution, and gradient elution is carried out within the eluent concentration range of 0-1 mol / L; in the step of gel chromatography purification, an eluent is a phosphate buffer solution with the pH value of 7.2-7.4, and the concentration of the eluent is 0.01-0.1 M; type III collagen with a complete triple helix structure and high biological activity is prepared from Tibetan sheep embryonic tissue, has the properties of good biocompatibility, good thermal stability, promotion of cell proliferation, adhesion and migration and the like, and has high medical value.

The invention relates to the technical field of organoid culture, and provides a mouse tooth embryo organoid culture method which comprises the following steps: step 1, taking a first molar tooth embryo of the lower jaw of an SD (Sprague Dawley) rat of which the embryo is 18.5 days; 2, dissociating the tooth embryo tissue into single cells by adopting a mechanical shearing and compound enzyme digestion method, wherein the compound enzyme is prepared by mixing collagenase I, neutral protease II and deoxyribonuclease I according to a mass ratio of 2: 2: 1; step 3, embedding the single-cell suspension in matrigel at a concentration of 150000 cells / 50 [mu] L; and 4, adding a DMEM / F-12 culture medium containing growth factors, and culturing for 7 days under the conditions of 37 DEG C and 5% CO2. The cultured organoid forms a layered structure similar to a clock-shaped tooth embryo on the seventh day, contains epithelial cells (CK14 positive) and mesenchymal cells (Vimentin positive), can express an enameblast marker AMELX, an odontoblast marker DSPP and a tooth development key factor TGF-beta, and is highly consistent with the in-vivo tooth embryo development characteristic.

This disclosure provides methods and compositions for increasing genome editing and site-directed integration events utilizing guided endonucleases and meiotic cell-preferred, egg cell-preferred or embryo tissue-preferred promoters.

The application discloses a kind of based on low-field nuclear magnetic resonance's poultry egg gender nondestructive identification method, belong to poultry egg detection technical field, comprising: S1, using low-field nuclear magnetic resonance equipment to carry out echo signal acquisition to poultry egg in turning chick period;S2, using non-negative least square optimization model to combine Tikhonov regular term to the echo signal of collection carries out spectrum inversion, reconstructs the T2 relaxation time spectrum related to poultry egg internal moisture and tissue structure distribution;S3, from T2 relaxation time spectrum, extract the multi-dimensional spectroscopy feature associated with poultry egg gender information, form the high-dimensional feature vector of representing embryo tissue microstructure difference;S4, high-dimensional feature vector is input into integrated classification model, and the output poultry egg gender identification result.The method of the application can be integrated on the automatic conveying line of hatching factory, realize multiple egg position parallel detection, fast non-contact identification and classification output, with high adaptability and engineering practicability.

The application discloses a seed propagation method of endangered plant Xymaloschka apetala. The application realizes quick and sufficient swelling of the seed within 3-5 hours by performing minimally invasive treatment on the seed coat of the seed hilum of Xymaloschka apetala, and awakens the seed vitality. The seed coat after swelling is easy to peel off without damaging the cotyledon and seed embryo tissue. After peeling off the seed coat, the seed is completely immersed in sterile water for 3-5 days, and the water is changed every 12 hours. After the seed radicle is swollen, the seed is immediately sowed into a plug, and the sowing adopts a "shallow sowing half cotyledon exposed method", the radicle is partially buried in the substrate, and the cotyledon is exposed half to contact air and light. The method realizes that the seed dormancy breaking to the initial seed germination of Xymaloschka apetala only needs 15 days, and the seedling embryo germination only needs 20 days and the seedling emergence rate reaches 98%. The method greatly shortens the seed propagation time of Xymaloschka apetala, improves the seed germination efficiency, and saves the seedling seed cost.

This utility model belongs to the field of embryo tissue culture technology, specifically relating to an intelligent sterile airflow barrier device for an embryo culture chamber. It includes a culture chamber with a vertical plate on the front and a horizontal plate on the top. The top edge of the vertical plate is fixedly connected to one side of an arc plate, the other side of the arc plate is fixedly connected to one side of the horizontal plate, the other side of the horizontal plate is fixedly connected to one side of an inclined plate, and the other side of the inclined plate is fixedly connected to the back of the culture chamber. This utility model can continuously blow clean the hands of operators when opening and closing the culture chamber door, effectively preventing contaminated air from directly entering the chamber; it can also effectively clean residual contaminants in the chamber cavity caused by eddies or dead corners; and it can save energy and extend the service life of the silent fan.

The utility model discloses an anti-pollution animal embryo transfer device. The anti-pollution animal embryo transfer device comprises a hose and a Barster suction tube, a filtering structure is arranged between the hose and the Barster suction pipe; the filtering structure comprises a filtering disc, filtering filler is arranged in the filtering disc, expansion pipes are arranged at the two ends of the filtering disc, connecting pipes are arranged at the two ends of each expansion pipe, one connecting pipe is in sealed connection with the hose, and the other connecting pipe is in sealed connection with the Barster suction pipe; the end, away from the Barster suction tube, of the hose is detachably connected with a mouth suction tube, the mouth suction tube is a soft tube, a degreasing cotton ball is arranged in the mouth suction tube, and a mouth suction part is arranged at the end of the mouth suction tube; and a pressure adjusting structure is arranged between the mouth suction pipe and the hose. According to the utility model, bacteria, saliva and other impurities carried in the mouth can be blocked when the embryo tissue is sucked by the mouth, so that the embryo is prevented from being polluted; the pressure adjusting assembly is arranged, the effective ventilation area of the mouth suction pipe can be changed, and therefore the pressure during mouth suction is adjusted.

This application relates to the field of biological tissue processing equipment technology, and discloses a closed-loop, high-efficiency tissue mincing device for extracting primary cells from chicken embryos. The device mainly includes a support frame, a cup body, a cooling box, a shearing assembly, and a sealing assembly. The shearing assembly is driven by magnetic coupling, with an external motor rotating a disk, which in turn drives a magnet at the bottom of the cup body to rotate a rotating shaft and moving blades. This creates an alternating shearing action with stationary blades fixed to the inner wall of the cup body, achieving uniform and efficient physical fragmentation of the chicken embryo tissue. The cooling assembly includes a cooling jacket surrounding the cup body and a circulating cooling system, effectively removing heat generated during the shearing process. The sealing assembly consists of a top cover, a sealing gasket, and a feed pipe with a sealing plug, ensuring a completely sealed operation. This invention solves the problems of significant mechanical damage to cells, easy thermal inactivation of samples, and the risk of contamination during open operation in existing technologies. It has the advantages of good cell morphology preservation, high biosafety, and convenient operation.

The invention provides a lotus embryo specific expression promoter pNnNF-YB1, a recombinant expression vector, recombinant bacteria and application, and belongs to the technical field of plant genetic engineering. According to the invention, a lotus embryo specific promoter pNnNF-YB1 located at the upstream 2011 bp of a specific high-expression gene NnNF-YB1 in a lotus seed cotyledon is found through gene sequencing. The promoter can drive a target gene to be highly expressed in a plant embryo, and has important scientific and application values for genetic improvement and quality improvement of a new variety of lotus; the method also can realize directional expression of a target gene in plant embryo tissues, can be applied to fundamental research of a plant development regulation mechanism, improvement of seed storage substance composition, guidance of crop genetic improvement and the like in the future, has great beneficial effects on scientific research and agricultural production, and also has a great significance in deep development and efficient utilization of plant gene resources. And beneficial reference is provided for application of plant genetic engineering in other species.

The application discloses a new type of maize haploid young embryo tissue culture doubling method and belongs to the field of plant breeding. The technical problem to be solved by the application is how to obtain a more simple maize haploid breeding method. To this end, the application provides a maize haploid young embryo chromosome doubling and seedling forming method, the method comprises the following steps: taking a maize inbred line as a female parent, taking a maize haploid induction line as a male parent to induce a maize young embryo, inoculating the maize young embryo into a double-layer culture medium for culture, and obtaining seedlings formed by chromosome doubling of the maize haploid young embryo; the double-layer culture medium is composed of a seedling forming culture medium and a doubling culture medium located on the seedling forming culture medium. The method provided by the application can complete chromosome doubling and seedling forming of the maize haploid young embryo in one step, and the maize young embryo does not need to be transferred to a seedling forming culture medium after chromosome doubling, so that a large amount of manpower is saved, and the method is expected to be used in production practice.