Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Method for keeping integrity of embryos in early embryo tissue immunohistochemistry

A technology of immunohistochemistry and integrity, which is applied in the preparation, sampling, and measuring devices of test samples, which can solve the problems of easy adhesion of embryos and rupture of cyst wall, and achieve simple operation, improved effect, and strong pertinence Effect

Inactive Publication Date: 2018-06-29
ANHUI SCI & TECH UNIV
View PDF7 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, during the culture process, the embryos are easy to adhere to the wall, and the capsule wall will be broken when the culture medium is changed to move the embryos, so many embryos will be lost during the whole culture process.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for keeping integrity of embryos in early embryo tissue immunohistochemistry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0023] The methods used in the following examples are conventional methods unless otherwise specified, and the percentages are volume percentages unless otherwise specified.

[0024] 1. Implementation materials

[0025] Early parthenogenetic embryos constructed in vitro, including precleavage, 2-cell embryos, 8-cell embryos and 16-cell embryos.

[0026] 2. Implementation method

[0027] 1) Solution preparation: 4% paraformaldehyde, 0.5% Triton X-100, hydrogen peroxide 20% polyvinylpyrrolidone storage solution and 0.1μg / mL 4',6-diamidino-2-phenylindole all use the pH range 7.2-7.4 0.01mol / L phosphate buffer dilution; and immunohistochemistry with 4% paraformaldehyde, 0.5% Triton X-100, hydrogen peroxide and phosphate buffer were added to polyvinylpyrrolidone stock solution, the added concentration 2%, 4%, 6%, 8%, 10% respectively;

[0028] 2) Embryo washing: Under a microscope, pick out the parthenogenetic embryos, androgenetic embryos or in vitro fertilized embryos cultivat...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for keeping the integrity of embryos in early embryo tissue immunohistochemistry, and belongs to the technical field of animals and organisms. A polyvinylpyrrolidone solution is added to a phosphate buffer solution, paraformaldehyde, Triton X-100 and hydrogen peroxide which are used for the early embryo tissue immunohistochemistry to prevent embryo attachment, so the integrity of the embryos is kept in the whole immunohistochemistry process, and the immunohistochemistry effect is improved. The method mainly aims at in vitro embryos, and the components of a tissue treatment reagent are adjusted on the basis of adjusting routine immunohistochemistry steps, so the immunohistochemistry effect is effectively improved. The method has the advantages of overcomingthe disadvantages in the immunohistochemistry technology, enlargement of the application range, simplicity in operation, and low production cost.

Description

technical field [0001] The invention relates to a method for maintaining embryo integrity in early embryo tissue immunohistochemistry, and belongs to the field of animal biotechnology. Background technique [0002] Polyvinylpyrrolidone, referred to as PVP, is a polymer compound produced by the polymerization of N-vinyl-2-pyrrolidone. It is a white hygroscopic powder, odorless or slightly odorous, soluble in water, ethanol, chloroform and most organic solvents, insoluble in ether, and less toxic. Polyvinylpyrrolidone can be synthesized by monomer vinylpyrrolidone through bulk polymerization, solution polymerization and other methods. As a synthetic water-soluble polymer compound, it has the general properties of water-soluble polymer compounds, colloidal protection, film-forming, cohesive, hygroscopic, solubilizing or coagulating effects. Its solution has very low toxicity and is non-physiologically descriptive. It is widely used in medicine, food, cosmetics and other fiel...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/533G01N1/30G01N1/34G01N1/28
CPCG01N1/30G01N1/34G01N33/533G01N2001/305
Inventor 王淑娟刘文举刘晓丽庞训胜闻爱友华金玲
Owner ANHUI SCI & TECH UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products