Method for keeping integrity of embryos in early embryo tissue immunohistochemistry
A technology of immunohistochemistry and integrity, which is applied in the preparation, sampling, and measuring devices of test samples, which can solve the problems of easy adhesion of embryos and rupture of cyst wall, and achieve simple operation, improved effect, and strong pertinence Effect
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[0023] The methods used in the following examples are conventional methods unless otherwise specified, and the percentages are volume percentages unless otherwise specified.
[0024] 1. Implementation materials
[0025] Early parthenogenetic embryos constructed in vitro, including precleavage, 2-cell embryos, 8-cell embryos and 16-cell embryos.
[0026] 2. Implementation method
[0027] 1) Solution preparation: 4% paraformaldehyde, 0.5% Triton X-100, hydrogen peroxide 20% polyvinylpyrrolidone storage solution and 0.1μg / mL 4',6-diamidino-2-phenylindole all use the pH range 7.2-7.4 0.01mol / L phosphate buffer dilution; and immunohistochemistry with 4% paraformaldehyde, 0.5% Triton X-100, hydrogen peroxide and phosphate buffer were added to polyvinylpyrrolidone stock solution, the added concentration 2%, 4%, 6%, 8%, 10% respectively;
[0028] 2) Embryo washing: Under a microscope, pick out the parthenogenetic embryos, androgenetic embryos or in vitro fertilized embryos cultivat...
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