8 results about "Immunohistochemistry technique" patented technology

The invention relates to an immune Raman multicolor imaging kit for liver cancer prediction and evaluation, the immune Raman multicolor imaging kit comprises five Raman probes, and the Raman probes are composed of Raman balls and antibodies connected to the surfaces of the Raman balls; wherein the Raman sphere is formed by polymerizing a triple bond monomer and (i) acrylic acid or (ii) styrene and acrylic acid, the particle size is 10-100 nm, and the Raman shift is 1800-2800 cm <-1 >; antibodies of the five Raman probes are respectively selected from one of an AFP antibody, an HBX antibody, a GPC3 antibody, a GS antibody and a CD34 antibody. The immune Raman multicolor imaging reagent and kit provided by the invention can realize one-step marking and multiple color development of five liver cancer related protein markers in the same liver tissue slice. Compared with a multiple fluorescence immunohistochemical technology, the method has remarkable advantages in efficiency and precision. Experiments show that the detection result is consistent with the clinical diagnosis result, and the application potential is huge.

The utility model relates to the technical field of cell climbing slide immunohistochemistry, and discloses a culture device for cell climbing slide immunohistochemistry, which comprises a culture plate monomer, a fixing component is arranged in the culture plate monomer, the top of the culture plate monomer is rotatably connected with a cover plate, the front side of the culture plate monomer is fixedly connected with a splicing block, and the splicing block is fixedly connected with the culture plate monomer. The tops of the culture plate single bodies are fixedly connected with a fixing shell, a splicing assembly is arranged in the fixing shell, and a clamping groove is formed in the cover plate; the fixing assembly comprises a connecting column, the connecting column is fixedly connected to the interior of the culture plate single body, the outer side of the connecting column is slidably connected with a sliding plate, and the periphery of the connecting column is sleeved with a first spring. According to the utility model, under the matching of the connecting rod, the spring I and the sliding plate, the clamping block is controlled to be clamped into the clamping groove, the position of the cover plate is fixed, only the cover plate of a single culture tank is opened, and other culture tanks are prevented from being polluted.

The application relates to the technical field of medical biological detection, and provides application of YAP protein asymmetric dimethylation modification at an arginine 124 site (YAP-R124me2a) in preparation of a tumor early diagnosis, prognosis evaluation or drug selection reagent or kit, wherein the methylation modification is asymmetric dimethylation modification, and the tumor is a YAP-related malignant solid tumor; further provided are a tumor early diagnosis, prognosis evaluation and drug selection kit and an evaluation system. The modification level of YAP-R124me2a in tumor tissue is determined by using an immunohistochemical technology, microscope photographing and computer image processing software, and combined with postoperative follow-up information, the correlation between the expression amount of YAP-R124me2a and the prognosis of tumor patients after surgery is determined, YAP-R124me2a can be used as a protein molecular marker for early diagnosis of liver cancer, lung adenocarcinoma, gastric cancer and colon cancer, judgment of postoperative prognosis and drug selection, and has important guiding significance for postoperative monitoring and individualized sequential treatment of tumor patients.

This invention relates to an immunoRaman multicolor imaging kit for the prediction and assessment of liver cancer. The immunoRaman multicolor imaging reagent contains five Raman probes, which are composed of Raman spheres and antibodies attached to the surface of the Raman spheres. The Raman spheres are polymerized from triple-bonded monomers with (i) acrylic acid or (ii) styrene and acrylic acid, with a particle size of 10–100 nm and a Raman shift of 1800–2800 cm⁻¹. ‑1 The antibodies used for the five Raman probes are selected from one of the following: AFP antibody, HBX antibody, GPC3 antibody, GS antibody, and CD34 antibody. The immunoRaman multicolor imaging reagent and kit provided by this invention can achieve one-step labeling and multiplex color development of five liver cancer-related protein markers in the same liver tissue section. Compared with multiplex fluorescence immunohistochemistry, this invention has significant advantages in both efficiency and accuracy. Experiments show that the detection results of this invention are consistent with clinical diagnostic results, demonstrating great application potential.

The invention relates to an immune cytochemical antigen repair liquid for liquid-based cells and a use method of the immune cytochemical antigen repair liquid, and belongs to the technical field of immunohistochemistry. The antigen repair liquid disclosed by the invention is prepared from tris (hydroxymethyl) aminomethane, ethylenediamine tetraacetic acid disodium salt, urea, boric acid, 2-methyl-4-isothiazolin-3-ketone, a nonionic surfactant, water and alkali metal hydroxide. The liquid-based cell antigen repairing liquid is good in repairing effect, repaired cell sheets are easy to dye, and the liquid-based cell antigen repairing liquid has the advantages of high immunohistochemical dyeing quality, accurate positive positioning, low non-specific background, clear background and good repeatability, safety and stability, so that the effects of improving the antigen detection rate, sterilizing and removing enzyme are achieved; therefore, the immunocytochemical staining of the liquid-based cytology sample can be adapted.

The invention belongs to the technical field of immunohistochemistry, and provides an immunohistochemistry detection kit which comprises a primary antibody, ferritin bionic manganese oxide nanoparticles and a developing solution, the primary antibody is a specific antibody of a target protein; the ferritin bionic manganese oxide nanoparticles are a compound of fusion ferritin and manganese oxide, and the fusion ferritin is fusion protein of ferritin and protein combined with an antibody Fc structural domain; the ferritin bionic manganese oxide nanoparticles are used for being combined with a primary antibody and catalyzing a substrate in a color developing solution to develop color; hydrogen peroxide is not included. According to the invention, the use of H2O2 can be avoided, and the problems of high detection cost, endogenous peroxidase interference and potential safety hazards caused by H2O2 in the existing immunohistochemical technology are solved.

The present application relates to the technical field of rapid immunohistochemistry, and particularly relates to a rapid immunohistochemical staining method for pleural effusion cytology. The method comprises the following steps: S1, taking the pleural effusion for processing to obtain cell sediment, drying the water of the cell group, and embedding; S2, placing the cell group on a freezing mold, wrapping with OCT embedding agent, and placing in a quick freezer to quickly freeze into a block, and then slicing; S3, taking several slices from the prepared cell group freezing block of S2, and using flowing water and PBS to clean the OCT embedding agent on the slices, and then placing the slices in a preheated citric acid buffer for medium-low fire repair; S4, after the slice repair is completed, using an OptiView DAB IHC Detection Kit to perform manual immunohistochemical staining on the slice. The rapid immunohistochemical staining technology for pleural effusion cytology established by the present application can obtain consistent diagnostic results with conventional paraffin methods within 2 hours, and provides reliable support for intraoperative rapid cytological pathological diagnosis.

The invention relates to the technical field of hepatocellular carcinoma detection, and discloses a postoperative circulating tumor cell detection method based on hepatocellular carcinoma. The method comprises the following steps: collecting a venous blood sample within half an hour after a hepatocellular carcinoma patient, treating the venous blood sample with a red blood cell lysis solution to remove red blood cell components, and performing centrifugal separation to obtain a cell precipitate; embedding the precipitate in a paraffin matrix to form a cell paraffin block, and continuously slicing to obtain a slice sample to be detected; labeling surface antigens of the circulating tumor cells in the slices through specific antibodies by adopting an immunohistochemical technology; collecting a microscopic image of the marked sample, and extracting morphological characteristic parameters and dyeing intensity distribution data related to the circulating tumor cells; a recognition model is constructed based on the data, and positive circulating tumor cells and background interference signals are distinguished. The method is standard in process, achieves efficient detection of circulating tumor cells, and meets clinical requirements of hepatocellular carcinoma postoperative monitoring.