4 results about "Immunohistochemistry technique" patented technology

This invention relates to an immunoRaman multicolor imaging kit for the prediction and assessment of liver cancer. The immunoRaman multicolor imaging reagent contains five Raman probes, which are composed of Raman spheres and antibodies attached to the surface of the Raman spheres. The Raman spheres are polymerized from triple-bonded monomers with (i) acrylic acid or (ii) styrene and acrylic acid, with a particle size of 10–100 nm and a Raman shift of 1800–2800 cm⁻¹. ‑1 The antibodies used for the five Raman probes are selected from one of the following: AFP antibody, HBX antibody, GPC3 antibody, GS antibody, and CD34 antibody. The immunoRaman multicolor imaging reagent and kit provided by this invention can achieve one-step labeling and multiplex color development of five liver cancer-related protein markers in the same liver tissue section. Compared with multiplex fluorescence immunohistochemistry, this invention has significant advantages in both efficiency and accuracy. Experiments show that the detection results of this invention are consistent with clinical diagnostic results, demonstrating great application potential.

The invention relates to an immune cytochemical antigen repair liquid for liquid-based cells and a use method of the immune cytochemical antigen repair liquid, and belongs to the technical field of immunohistochemistry. The antigen repair liquid disclosed by the invention is prepared from tris (hydroxymethyl) aminomethane, ethylenediamine tetraacetic acid disodium salt, urea, boric acid, 2-methyl-4-isothiazolin-3-ketone, a nonionic surfactant, water and alkali metal hydroxide. The liquid-based cell antigen repairing liquid is good in repairing effect, repaired cell sheets are easy to dye, and the liquid-based cell antigen repairing liquid has the advantages of high immunohistochemical dyeing quality, accurate positive positioning, low non-specific background, clear background and good repeatability, safety and stability, so that the effects of improving the antigen detection rate, sterilizing and removing enzyme are achieved; therefore, the immunocytochemical staining of the liquid-based cytology sample can be adapted.

The present application relates to the technical field of rapid immunohistochemistry, and particularly relates to a rapid immunohistochemical staining method for pleural effusion cytology. The method comprises the following steps: S1, taking the pleural effusion for processing to obtain cell sediment, drying the water of the cell group, and embedding; S2, placing the cell group on a freezing mold, wrapping with OCT embedding agent, and placing in a quick freezer to quickly freeze into a block, and then slicing; S3, taking several slices from the prepared cell group freezing block of S2, and using flowing water and PBS to clean the OCT embedding agent on the slices, and then placing the slices in a preheated citric acid buffer for medium-low fire repair; S4, after the slice repair is completed, using an OptiView DAB IHC Detection Kit to perform manual immunohistochemical staining on the slice. The rapid immunohistochemical staining technology for pleural effusion cytology established by the present application can obtain consistent diagnostic results with conventional paraffin methods within 2 hours, and provides reliable support for intraoperative rapid cytological pathological diagnosis.

The invention relates to the technical field of hepatocellular carcinoma detection, and discloses a postoperative circulating tumor cell detection method based on hepatocellular carcinoma. The method comprises the following steps: collecting a venous blood sample within half an hour after a hepatocellular carcinoma patient, treating the venous blood sample with a red blood cell lysis solution to remove red blood cell components, and performing centrifugal separation to obtain a cell precipitate; embedding the precipitate in a paraffin matrix to form a cell paraffin block, and continuously slicing to obtain a slice sample to be detected; labeling surface antigens of the circulating tumor cells in the slices through specific antibodies by adopting an immunohistochemical technology; collecting a microscopic image of the marked sample, and extracting morphological characteristic parameters and dyeing intensity distribution data related to the circulating tumor cells; a recognition model is constructed based on the data, and positive circulating tumor cells and background interference signals are distinguished. The method is standard in process, achieves efficient detection of circulating tumor cells, and meets clinical requirements of hepatocellular carcinoma postoperative monitoring.